Sequence of pcDNA4_His-Max

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Sequence of pcDNA4_His-Max_A

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Sequence of pcDNA4/His-Max A : 5258 BP; 1218 A; 1367 C; 1371 G; 1302 T; 0 other;

.......... BglII(12) .......... .......... .......... ..........

1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 60

1 ctgcctagcc ctctagaggg ctaggggata ccagctgaga gtcatgttag acgagactac 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120

61 ggcgtatcaa ttcggtcata gacgagggac gaacacacaa cctccagcga ctcatcacgc 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180

121 gctcgtttta aattcgatgt tgttccgttc cgaactggct gttaacgtac ttcttagacg 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240

181 aatcccaatc cgcaaaacgc gacgaagcgc tacatgcccg gtctatatgc gcaactgtaa 240

.......... .......... .......... .......... .......... ..........

SpeI(249) .......... .......... .......... .......... ..........

241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 300

241 ctaataactg atcaataatt atcattagtt aatgccccag taatcaagta tcgggtatat 300

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 360

301 acctcaaggc gcaatgtatt gaatgccatt taccgggcgg accgactggc gggttgctgg 360

.......... ....... CMV_immearly_pro CAG_enh ....... ..........

.......... .......... .......... .......... .......... ..........

361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 420

361 gggcgggtaa ctgcagttat tactgcatac aagggtatca ttgcggttat ccctgaaagg 420

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 480

421 taactgcagt tacccacctg ataaatgcca tttgacgggt gaaccgtcat gtagttcaca 480

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

NdeI(484) .......... .......... .......... .......... ..........

481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 540

481 tagtatacgg ttcatgcggg ggataactgc agttactgcc atttaccggg cggaccgtaa 540

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 600

541 tacgggtcat gtactggaat accctgaaag gatgaaccgt catgtagatg cataatcagt 600

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 660

601 agcgataatg gtaccactac gccaaaaccg tcatgtagtt acccgcacct atcgccaaac 660

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 720

661 tgagtgcccc taaaggttca gaggtggggt aactgcagtt accctcaaac aaaaccgtgg 720

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 780

721 ttttagttgc cctgaaaggt tttacagcat tgttgaggcg gggtaactgc gtttacccgc 780

.......... ....... CMV_immearly_pro ....... ......... CMV_fwd_pri

.......... .......... .......... .......... .......... ..........

781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 840

781 catccgcaca tgccaccctc cagatatatt cgtctcgaga gaccgattga tctcttgggt 840

CMV_fwd_pri ..... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 900

841 gacgaatgac cgaatagctt taattatgct gagtgatatc cctctgggtt cgaccgatcg 900

CMV_immearly_pro .... .......... T7_pro .......... ..........

... AflII(908),HindIII(911) ... .......... .......... ..........

901 gtttaaactt aagcttagcg cagaggcttg gggcagccga gcggcagcca ggccccggcc 960

901 caaatttgaa ttcgaatcgc gtctccgaac cccgtcggct cgccgtcggt ccggggccgg 960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 cgggcctcgg ttccagaagg gagaggagcc cgccaaggcg cgcaagagag cgggctgcct 1020

961 gcccggagcc aaggtcttcc ctctcctcgg gcggttccgc gcgttctctc gcccgacgga 1020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 cgcagtccga gccggagagg gagcgcgagc cgcgccggcc ccggacggcc tccgaaacca 1080

1021 gcgtcaggct cggcctctcc ctcgcgctcg gcgcggccgg ggcctgccgg aggctttggt 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 tggggggttc tcatcatcat catcatcatg gtatggctag catgactggt ggacagcaaa 1140

1081 accccccaag agtagtagta gtagtagtac cataccgatc gtactgacca cctgtcgttt 1140

.......... 6xHis .... Xpress_fwd_pri T7_gene10_leader T7_leader

.......... .......... .... KpnI(1176),BamHI(1180) .... ..........

1141 tgggtcggga tctgtacgac gatgacgata aggtacctag gatccagtgt ggtggaattc 1200

1141 acccagccct agacatgctg ctactgctat tccatggatc ctaggtcaca ccaccttaag 1200

.......... Xpress_EK .......... .......... .......... ..........

PstI,EcoRV NotI) XhoI(1228) XbaI(1234) ApaI(1244)

1201 tgcagatatc cagcacagtg gcggccgctc gagtctagag ggcccgttta aacccgctga 1260

1201 acgtctatag gtcgtgtcac cgccggcgag ctcagatctc ccgggcaaat ttgggcgact 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct 1320

1261 agtcggagct gacacggaag atcaacggtc ggtagacaac aaacggggag ggggcacgga 1320

......... BGH_rev_pri ........ bGH_PA_term ......... ..........

.......... .......... .......... .......... .......... ..........

1321 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca 1380

1321 aggaactggg accttccacg gtgagggtga caggaaagga ttattttact cctttaacgt 1380

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag 1440

1381 agcgtaacag actcatccac agtaagataa gaccccccac cccaccccgt cctgtcgttc 1440

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct 1500

1441 cccctcctaa cccttctgtt atcgtccgta cgacccctac gccacccgag ataccgaaga 1500

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca 1560

1501 ctccgccttt cttggtcgac cccgagatcc cccatagggg tgcgcgggac atcgccgcgt 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta 1620

1561 aattcgcgcc gcccacacca ccaatgcgcg tcgcactggc gatgtgaacg gtcgcgggat 1620

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt 1680

1621 cgcgggcgag gaaagcgaaa gaagggaagg aaagagcggt gcaagcggcc gaaaggggca 1680

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 caagctctaa atcggggcat ccctttaggg ttccgattta gtgctttacg gcacctcgac 1740

1681 gttcgagatt tagccccgta gggaaatccc aaggctaaat cacgaaatgc cgtggagctg 1740

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt 1800

1741 gggttttttg aactaatccc actaccaagt gcatcacccg gtagcgggac tatctgccaa 1800

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga 1860

1801 aaagcgggaa actgcaacct caggtgcaag aaattatcac ctgagaacaa ggtttgacct 1860

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 acaacactca accctatctc ggtctattct tttgatttat aagggatttt ggggatttcg 1920

1861 tgttgtgagt tgggatagag ccagataaga aaactaaata ttccctaaaa cccctaaagc 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga 1980

1921 cggataacca attttttact cgactaaatt gtttttaaat tgcgcttaat taagacacct 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc aggcaggcag aagtatgcaa 2040

1981 tacacacagt caatcccaca cctttcaggg gtccgagggg tccgtccgtc ttcatacgtt 2040

pBABE_3_pri ........ ...... SV40_enh SV40_pro ...... ..........

.......... .......... .......... .......... .......... ..........

2041 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc 2100

2041 tcgtacgtag agttaatcag tcgttggtcc acacctttca ggggtccgag gggtcgtccg 2100

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg 2160

2101 tcttcatacg tttcgtacgt agagttaatc agtcgttggt atcagggcgg ggattgaggc 2160

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt 2220

2161 gggtagggcg gggattgagg cgggtcaagg cgggtaagag gcggggtacc gactgattaa 2220

......... SV40_enh SV40_pro SV40_origin ......... ..........

.......... .......... .......... .......... .......... ..........

2221 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga 2280

2221 aaaaaataaa tacgtctccg gctccggcgg agacggagac tcgataaggt cttcatcact 2280

.. SV40_origin SV40pro_F_pri SV40_pro .. .......... ..........

.......... .......... .......... .......... .......... ..........

2281 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt 2340

2281 cctccgaaaa aacctccgga tccgaaaacg tttttcgagg gccctcgaac atataggtaa 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 ttcggatctg atcagcacgt gttgacaatt aatcatcggc atagtatatc ggcatagtat 2400

2341 aagcctagac tagtcgtgca caactgttaa ttagtagccg tatcatatag ccgtatcata 2400

.......... .......... .......... EM7_pro .......... ..........

.......... .......... MscI(2430) .......... .......... ..........

2401 aatacgacaa ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt ccggtgctca 2460

2401 ttatgctgtt ccactccttg atttggtacc ggttcaactg gtcacggcaa ggccacgagt 2460

.......... EM7_pro .......... ......... bleo sh_ble .........

.......... .......... .......... .......... .......... ..........

2461 ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga ccggctcggg ttctcccggg 2520

2461 ggcgcgcgct gcagcggcct cgccagctca agacctggct ggccgagccc aagagggccc 2520

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 acttcgtgga ggacgacttc gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg 2580

2521 tgaagcacct cctgctgaag cggccacacc aggccctgct gcactgggac aagtagtcgc 2580

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 cggtccagga ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg 2640

2581 gccaggtcct ggtccaccac ggcctgttgt gggaccggac ccacacccac gcgccggacc 2640

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac gcctccgggc 2700

2641 tgctcgacat gcggctcacc agcctccagc acaggtgctt gaaggccctg cggaggcccg 2700

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 cggccatgac cgagatcggc gagcagccgt gggggcggga gttcgccctg cgcgacccgg 2760

2701 gccggtactg gctctagccg ctcgtcggca cccccgccct caagcgggac gcgctgggcc 2760

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 ccggcaactg cgtgcacttc gtggccgagg agcaggactg acacgtgcta cgagatttcg 2820

2761 ggccgttgac gcacgtgaag caccggctcc tcgtcctgac tgtgcacgat gctctaaagc 2820

.......... bleo sh_ble .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct 2880

2821 taaggtggcg gcggaagata ctttccaacc cgaagcctta gcaaaaggcc ctgcggccga 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta 2940

2881 cctactagga ggtcgcgccc ctagagtacg acctcaagaa gcgggtgggg ttgaacaaat 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat 3000

2941 aacgtcgaat attaccaatg tttatttcgt tatcgtagtg tttaaagtgt ttatttcgta 3000

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct 3060

3001 aaaaaagtga cgtaagatca acaccaaaca ggtttgagta gttacataga atagtacaga 3060

.......... ........ SV40_PA_term EBV_rev_pri ........ ..........

.......... .......... .......... .......... .......... ..........

3061 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt 3120

3061 catatggcag ctggagatcg atctcgaacc gcattagtac cagtatcgac aaaggacaca 3120

.......... .......... .......... ....... M13_reverse_pri .......

.......... .......... .......... .......... .......... ..........

3121 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag 3180

3121 ctttaacaat aggcgagtgt taaggtgtgt tgtatgctcg gccttcgtat ttcacatttc 3180

M13_pUC_rev_pri .... .......... .......... lac_pro ..........

.......... .......... .......... .......... .......... ..........

3181 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt 3240

3181 ggaccccacg gattactcac tcgattgagt gtaattaacg caacgcgagt gacgggcgaa 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag 3300

3241 aggtcagccc tttggacagc acggtcgacg taattactta gccggttgcg cgcccctctc 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 3360

3301 cgccaaacgc ataacccgcg agaaggcgaa ggagcgagtg actgagcgac gcgagccagc 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 3420

3361 aagccgacgc cgctcgccat agtcgagtga gtttccgcca ttatgccaat aggtgtctta 3420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 3480

3421 gtcccctatt gcgtcctttc ttgtacactc gttttccggt cgttttccgg tccttggcat 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 3540

3481 ttttccggcg caacgaccgc aaaaaggtat ccgaggcggg gggactgctc gtagtgtttt 3540

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

3541 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 3600

3541 tagctgcgag ttcagtctcc accgctttgg gctgtcctga tatttctatg gtccgcaaag 3600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3601 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 3660

3601 ggggaccttc gagggagcac gcgagaggac aaggctggga cggcgaatgg cctatggaca 3660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 ccgcctttct cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca 3720

3661 ggcggaaaga gggaagccct tcgcaccgcg aaagagttac gagtgcgaca tccatagagt 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 3780

3721 caagccacat ccagcaagcg aggttcgacc cgacacacgt gcttgggggg caagtcgggc 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 3840

3781 tggcgacgcg gaataggcca ttgatagcag aactcaggtt gggccattct gtgctgaata 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 3900

3841 gcggtgaccg tcgtcggtga ccattgtcct aatcgtctcg ctccatacat ccgccacgat 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct 3960

3901 gtctcaagaa cttcaccacc ggattgatgc cgatgtgatc ttcctgtcat aaaccataga 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 4020

3961 cgcgagacga cttcggtcaa tggaagcctt tttctcaacc atcgagaact aggccgtttg 4020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 4080

4021 tttggtggcg accatcgcca ccaaaaaaac aaacgttcgt cgtctaatgc gcgtcttttt 4080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 4140

4081 ttcctagagt tcttctagga aactagaaaa gatgccccag actgcgagtc accttgcttt 4140

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 4200

4141 tgagtgcaat tccctaaaac cagtactcta atagtttttc ctagaagtgg atctaggaaa 4200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 4260

4201 atttaatttt tacttcaaaa tttagttaga tttcatatat actcatttga accagactgt 4260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca 4320

4261 caatggttac gaattagtca ctccgtggat agagtcgcta gacagataaa gcaagtaggt 4320

.......... .......... ....... ORF 2 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

4321 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc 4380

4321 atcaacggac tgaggggcag cacatctatt gatgctatgc cctcccgaat ggtagaccgg 4380

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa 4440

4381 ggtcacgacg ttactatggc gctctgggtg cgagtggccg aggtctaaat agtcgttatt 4440

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc 4500

4441 tggtcggtcg gccttcccgg ctcgcgtctt caccaggacg ttgaaatagg cggaggtagg 4500

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... FspI(4557)

4501 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca 4560

4501 tcagataatt aacaacggcc cttcgatctc attcatcaag cggtcaatta tcaaacgcgt 4560

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat 4620

4561 tgcaacaacg gtaacgatgt ccgtagcacc acagtgcgag cagcaaacca taccgaagta 4620

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag 4680

4621 agtcgaggcc aagggttgct agttccgctc aatgtactag ggggtacaac acgttttttc 4680

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac 4740

4681 gccaatcgag gaagccagga ggctagcaac agtcttcatt caaccggcgt cacaatagtg 4740

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt 4800

4741 agtaccaata ccgtcgtgac gtattaagag aatgacagta cggtaggcat tctacgaaaa 4800

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt 4860

4801 gacactgacc actcatgagt tggttcagta agactcttat cacatacgcc gctggctcaa 4860

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc 4920

4861 cgagaacggg ccgcagttat gccctattat ggcgcggtgt atcgtcttga aattttcacg 4920

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat 4980

4921 agtagtaacc ttttgcaaga agccccgctt ttgagagttc ctagaatggc gacaactcta 4980

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca 5040

4981 ggtcaagcta cattgggtga gcacgtgggt tgactagaag tcgtagaaaa tgaaagtggt 5040

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga 5100

5041 cgcaaagacc cactcgtttt tgtccttccg ttttacggcg ttttttccct tattcccgct 5100

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg 5160

5101 gtgcctttac aacttatgag tatgagaagg aaaaagttat aataacttcg taaatagtcc 5160

....... ORF 2 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg 5220

5161 caataacaga gtactcgcct atgtataaac ttacataaat ctttttattt gtttatcccc 5220

.......... AmpR_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 ttccgcgcac atttccccga aaagtgccac ctgacgtc.. .......... .......... 5258

5221 aaggcgcgtg taaaggggct tttcacggtg gactgcag.. .......... .......... 5258

.......... .......... .......... .......... .......... ..........