Sequence of pcDNA4_His-Max

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Sequence of pcDNA4_His-Max_B

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Sequence of pcDNA4/His-Max B : 5259 BP; 1219 A; 1367 C; 1371 G; 1302 T; 0 other;

.......... BglII(12) .......... .......... .......... ..........

1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 60

1 ctgcctagcc ctctagaggg ctaggggata ccagctgaga gtcatgttag acgagactac 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120

61 ggcgtatcaa ttcggtcata gacgagggac gaacacacaa cctccagcga ctcatcacgc 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180

121 gctcgtttta aattcgatgt tgttccgttc cgaactggct gttaacgtac ttcttagacg 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240

181 aatcccaatc cgcaaaacgc gacgaagcgc tacatgcccg gtctatatgc gcaactgtaa 240

.......... .......... .......... .......... .......... ..........

SpeI(249) .......... .......... .......... .......... ..........

241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 300

241 ctaataactg atcaataatt atcattagtt aatgccccag taatcaagta tcgggtatat 300

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 360

301 acctcaaggc gcaatgtatt gaatgccatt taccgggcgg accgactggc gggttgctgg 360

.......... ....... CMV_immearly_pro CAG_enh ....... ..........

.......... .......... .......... .......... .......... ..........

361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 420

361 gggcgggtaa ctgcagttat tactgcatac aagggtatca ttgcggttat ccctgaaagg 420

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 480

421 taactgcagt tacccacctg ataaatgcca tttgacgggt gaaccgtcat gtagttcaca 480

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

NdeI(484) .......... .......... .......... .......... ..........

481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 540

481 tagtatacgg ttcatgcggg ggataactgc agttactgcc atttaccggg cggaccgtaa 540

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 600

541 tacgggtcat gtactggaat accctgaaag gatgaaccgt catgtagatg cataatcagt 600

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 660

601 agcgataatg gtaccactac gccaaaaccg tcatgtagtt acccgcacct atcgccaaac 660

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 720

661 tgagtgcccc taaaggttca gaggtggggt aactgcagtt accctcaaac aaaaccgtgg 720

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 780

721 ttttagttgc cctgaaaggt tttacagcat tgttgaggcg gggtaactgc gtttacccgc 780

.......... ....... CMV_immearly_pro ....... ......... CMV_fwd_pri

.......... .......... .......... .......... .......... ..........

781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 840

781 catccgcaca tgccaccctc cagatatatt cgtctcgaga gaccgattga tctcttgggt 840

CMV_fwd_pri ..... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 900

841 gacgaatgac cgaatagctt taattatgct gagtgatatc cctctgggtt cgaccgatcg 900

CMV_immearly_pro .... .......... T7_pro .......... ..........

... AflII(908),HindIII(911) ... .......... .......... ..........

901 gtttaaactt aagcttagcg cagaggcttg gggcagccga gcggcagcca ggccccggcc 960

901 caaatttgaa ttcgaatcgc gtctccgaac cccgtcggct cgccgtcggt ccggggccgg 960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 cgggcctcgg ttccagaagg gagaggagcc cgccaaggcg cgcaagagag cgggctgcct 1020

961 gcccggagcc aaggtcttcc ctctcctcgg gcggttccgc gcgttctctc gcccgacgga 1020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 cgcagtccga gccggagagg gagcgcgagc cgcgccggcc ccggacggcc tccgaaacca 1080

1021 gcgtcaggct cggcctctcc ctcgcgctcg gcgcggccgg ggcctgccgg aggctttggt 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 tggggggttc tcatcatcat catcatcatg gtatggctag catgactggt ggacagcaaa 1140

1081 accccccaag agtagtagta gtagtagtac cataccgatc gtactgacca cctgtcgttt 1140

.......... 6xHis .... Xpress_fwd_pri T7_gene10_leader T7_leader

.......... .......... .......... .... KpnI(1176),BamHI(1181) ....

1141 tgggtcggga tctgtacgac gatgacgata aggtacctaa ggatccagtg tggtggaatt 1200

1141 acccagccct agacatgctg ctactgctat tccatggatt cctaggtcac accaccttaa 1200

.......... Xpress_EK .......... .......... .......... ..........

PstI,EcoRV NotI) XhoI(1229) XbaI(1235) ApaI(1245)

1201 ctgcagatat ccagcacagt ggcggccgct cgagtctaga gggcccgttt aaacccgctg 1260

1201 gacgtctata ggtcgtgtca ccgccggcga gctcagatct cccgggcaaa tttgggcgac 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc 1320

1261 tagtcggagc tgacacggaa gatcaacggt cggtagacaa caaacgggga gggggcacgg 1320

......... BGH_rev_pri ........ bGH_PA_term ......... ..........

.......... .......... .......... .......... .......... ..........

1321 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc 1380

1321 aaggaactgg gaccttccac ggtgagggtg acaggaaagg attattttac tcctttaacg 1380

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa 1440

1381 tagcgtaaca gactcatcca cagtaagata agacccccca ccccaccccg tcctgtcgtt 1440

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc 1500

1441 ccccctccta acccttctgt tatcgtccgt acgaccccta cgccacccga gataccgaag 1500

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc 1560

1501 actccgcctt tcttggtcga ccccgagatc ccccataggg gtgcgcggga catcgccgcg 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct 1620

1561 taattcgcgc cgcccacacc accaatgcgc gtcgcactgg cgatgtgaac ggtcgcggga 1620

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg 1680

1621 tcgcgggcga ggaaagcgaa agaagggaag gaaagagcgg tgcaagcggc cgaaaggggc 1680

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 tcaagctcta aatcggggca tccctttagg gttccgattt agtgctttac ggcacctcga 1740

1681 agttcgagat ttagccccgt agggaaatcc caaggctaaa tcacgaaatg ccgtggagct 1740

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt 1800

1741 ggggtttttt gaactaatcc cactaccaag tgcatcaccc ggtagcggga ctatctgcca 1800

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg 1860

1801 aaaagcggga aactgcaacc tcaggtgcaa gaaattatca cctgagaaca aggtttgacc 1860

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tggggatttc 1920

1861 ttgttgtgag ttgggataga gccagataag aaaactaaat attccctaaa acccctaaag 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg 1980

1921 ccggataacc aattttttac tcgactaaat tgtttttaaa ttgcgcttaa ttaagacacc 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc caggcaggca gaagtatgca 2040

1981 ttacacacag tcaatcccac acctttcagg ggtccgaggg gtccgtccgt cttcatacgt 2040

pBABE_3_pri ........ ...... SV40_enh SV40_pro ...... ..........

.......... .......... .......... .......... .......... ..........

2041 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg 2100

2041 ttcgtacgta gagttaatca gtcgttggtc cacacctttc aggggtccga ggggtcgtcc 2100

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc 2160

2101 gtcttcatac gtttcgtacg tagagttaat cagtcgttgg tatcagggcg gggattgagg 2160

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat 2220

2161 cgggtagggc ggggattgag gcgggtcaag gcgggtaaga ggcggggtac cgactgatta 2220

.......... ..... SV40_enh SV40_pro SV40_origin ..... ..........

.......... .......... .......... .......... .......... ..........

2221 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg 2280

2221 aaaaaaataa atacgtctcc ggctccggcg gagacggaga ctcgataagg tcttcatcac 2280

.. SV40_origin SV40pro_F_pri SV40_pro .. .......... ..........

.......... .......... .......... .......... .......... ..........

2281 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat 2340

2281 tcctccgaaa aaacctccgg atccgaaaac gtttttcgag ggccctcgaa catataggta 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 tttcggatct gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta 2400

2341 aaagcctaga ctagtcgtgc acaactgtta attagtagcc gtatcatata gccgtatcat 2400

.......... .......... .......... EM7_pro .......... ..........

.......... .......... .......... MscI(2431) .......... ..........

2401 taatacgaca aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt tccggtgctc 2460

2401 attatgctgt tccactcctt gatttggtac cggttcaact ggtcacggca aggccacgag 2460

.......... EM7_pro .......... ......... bleo sh_ble .........

.......... .......... .......... .......... .......... ..........

2461 accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg 2520

2461 tggcgcgcgc tgcagcggcc tcgccagctc aagacctggc tggccgagcc caagagggcc 2520

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc 2580

2521 ctgaagcacc tcctgctgaa gcggccacac caggccctgc tgcactggga caagtagtcg 2580

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg 2640

2581 cgccaggtcc tggtccacca cggcctgttg tgggaccgga cccacaccca cgcgccggac 2640

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg 2700

2641 ctgctcgaca tgcggctcac cagcctccag cacaggtgct tgaaggccct gcggaggccc 2700

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg 2760

2701 ggccggtact ggctctagcc gctcgtcggc acccccgccc tcaagcggga cgcgctgggc 2760

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 gccggcaact gcgtgcactt cgtggccgag gagcaggact gacacgtgct acgagatttc 2820

2761 cggccgttga cgcacgtgaa gcaccggctc ctcgtcctga ctgtgcacga tgctctaaag 2820

.......... bleo sh_ble .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc 2880

2821 ctaaggtggc ggcggaagat actttccaac ccgaagcctt agcaaaaggc cctgcggccg 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt 2940

2881 acctactagg aggtcgcgcc cctagagtac gacctcaaga agcgggtggg gttgaacaaa 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca 3000

2941 taacgtcgaa tattaccaat gtttatttcg ttatcgtagt gtttaaagtg tttatttcgt 3000

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc 3060

3001 aaaaaaagtg acgtaagatc aacaccaaac aggtttgagt agttacatag aatagtacag 3060

.......... ........ SV40_PA_term EBV_rev_pri ........ ..........

.......... .......... .......... .......... .......... ..........

3061 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg 3120

3061 acatatggca gctggagatc gatctcgaac cgcattagta ccagtatcga caaaggacac 3120

.......... .......... .......... ....... M13_reverse_pri .......

.......... .......... .......... .......... .......... ..........

3121 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa 3180

3121 actttaacaa taggcgagtg ttaaggtgtg ttgtatgctc ggccttcgta tttcacattt 3180

M13_pUC_rev_pri .... .......... .......... lac_pro ..........

.......... .......... .......... .......... .......... ..........

3181 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct 3240

3181 cggaccccac ggattactca ctcgattgag tgtaattaac gcaacgcgag tgacgggcga 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga 3300

3241 aaggtcagcc ctttggacag cacggtcgac gtaattactt agccggttgc gcgcccctct 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc 3360

3301 ccgccaaacg cataacccgc gagaaggcga aggagcgagt gactgagcga cgcgagccag 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa 3420

3361 caagccgacg ccgctcgcca tagtcgagtg agtttccgcc attatgccaa taggtgtctt 3420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 3480

3421 agtcccctat tgcgtccttt cttgtacact cgttttccgg tcgttttccg gtccttggca 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 3540

3481 tttttccggc gcaacgaccg caaaaaggta tccgaggcgg ggggactgct cgtagtgttt 3540

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

3541 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 3600

3541 ttagctgcga gttcagtctc caccgctttg ggctgtcctg atatttctat ggtccgcaaa 3600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3601 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 3660

3601 gggggacctt cgagggagca cgcgagagga caaggctggg acggcgaatg gcctatggac 3660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 tccgcctttc tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc 3720

3661 aggcggaaag agggaagccc ttcgcaccgc gaaagagtta cgagtgcgac atccatagag 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 3780

3721 tcaagccaca tccagcaagc gaggttcgac ccgacacacg tgcttggggg gcaagtcggg 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 3840

3781 ctggcgacgc ggaataggcc attgatagca gaactcaggt tgggccattc tgtgctgaat 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 3900

3841 agcggtgacc gtcgtcggtg accattgtcc taatcgtctc gctccataca tccgccacga 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc 3960

3901 tgtctcaaga acttcaccac cggattgatg ccgatgtgat cttcctgtca taaaccatag 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 4020

3961 acgcgagacg acttcggtca atggaagcct ttttctcaac catcgagaac taggccgttt 4020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 4080

4021 gtttggtggc gaccatcgcc accaaaaaaa caaacgttcg tcgtctaatg cgcgtctttt 4080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 4140

4081 tttcctagag ttcttctagg aaactagaaa agatgcccca gactgcgagt caccttgctt 4140

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 4200

4141 ttgagtgcaa ttccctaaaa ccagtactct aatagttttt cctagaagtg gatctaggaa 4200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 4260

4201 aatttaattt ttacttcaaa atttagttag atttcatata tactcatttg aaccagactg 4260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 4320

4261 tcaatggtta cgaattagtc actccgtgga tagagtcgct agacagataa agcaagtagg 4320

.......... .......... ....... ORF 3 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

4321 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc 4380

4321 tatcaacgga ctgaggggca gcacatctat tgatgctatg ccctcccgaa tggtagaccg 4380

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata 4440

4381 gggtcacgac gttactatgg cgctctgggt gcgagtggcc gaggtctaaa tagtcgttat 4440

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 4500

4441 ttggtcggtc ggccttcccg gctcgcgtct tcaccaggac gttgaaatag gcggaggtag 4500

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... FspI(4558)

4501 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 4560

4501 gtcagataat taacaacggc ccttcgatct cattcatcaa gcggtcaatt atcaaacgcg 4560

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 4620

4561 ttgcaacaac ggtaacgatg tccgtagcac cacagtgcga gcagcaaacc ataccgaagt 4620

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 4680

4621 aagtcgaggc caagggttgc tagttccgct caatgtacta gggggtacaa cacgtttttt 4680

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 4740

4681 cgccaatcga ggaagccagg aggctagcaa cagtcttcat tcaaccggcg tcacaatagt 4740

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 4800

4741 gagtaccaat accgtcgtga cgtattaaga gaatgacagt acggtaggca ttctacgaaa 4800

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 4860

4801 agacactgac cactcatgag ttggttcagt aagactctta tcacatacgc cgctggctca 4860

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg 4920

4861 acgagaacgg gccgcagtta tgccctatta tggcgcggtg tatcgtcttg aaattttcac 4920

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 4980

4921 gagtagtaac cttttgcaag aagccccgct tttgagagtt cctagaatgg cgacaactct 4980

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 5040

4981 aggtcaagct acattgggtg agcacgtggg ttgactagaa gtcgtagaaa atgaaagtgg 5040

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 5100

5041 tcgcaaagac ccactcgttt ttgtccttcc gttttacggc gttttttccc ttattcccgc 5100

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 5160

5101 tgtgccttta caacttatga gtatgagaag gaaaaagtta taataacttc gtaaatagtc 5160

....... ORF 3 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 5220

5161 ccaataacag agtactcgcc tatgtataaa cttacataaa tctttttatt tgtttatccc 5220

.......... AmpR_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 gttccgcgca catttccccg aaaagtgcca cctgacgtc. .......... .......... 5259

5221 caaggcgcgt gtaaaggggc ttttcacggt ggactgcag. .......... .......... 5259

.......... .......... .......... .......... .......... ..........