Sequence of pcDNA4_His-Max

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Sequence of pcDNA4_His-Max_C

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Sequence of pcDNA4/His-Max C : 5257 BP; 1218 A; 1367 C; 1371 G; 1301 T; 0 other;

.......... BglII(12) .......... .......... .......... ..........

1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 60

1 ctgcctagcc ctctagaggg ctaggggata ccagctgaga gtcatgttag acgagactac 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120

61 ggcgtatcaa ttcggtcata gacgagggac gaacacacaa cctccagcga ctcatcacgc 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180

121 gctcgtttta aattcgatgt tgttccgttc cgaactggct gttaacgtac ttcttagacg 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240

181 aatcccaatc cgcaaaacgc gacgaagcgc tacatgcccg gtctatatgc gcaactgtaa 240

.......... .......... .......... .......... .......... ..........

SpeI(249) .......... .......... .......... .......... ..........

241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 300

241 ctaataactg atcaataatt atcattagtt aatgccccag taatcaagta tcgggtatat 300

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 360

301 acctcaaggc gcaatgtatt gaatgccatt taccgggcgg accgactggc gggttgctgg 360

.......... ....... CMV_immearly_pro CAG_enh ....... ..........

.......... .......... .......... .......... .......... ..........

361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 420

361 gggcgggtaa ctgcagttat tactgcatac aagggtatca ttgcggttat ccctgaaagg 420

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 480

421 taactgcagt tacccacctg ataaatgcca tttgacgggt gaaccgtcat gtagttcaca 480

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

NdeI(484) .......... .......... .......... .......... ..........

481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 540

481 tagtatacgg ttcatgcggg ggataactgc agttactgcc atttaccggg cggaccgtaa 540

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 600

541 tacgggtcat gtactggaat accctgaaag gatgaaccgt catgtagatg cataatcagt 600

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 660

601 agcgataatg gtaccactac gccaaaaccg tcatgtagtt acccgcacct atcgccaaac 660

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 720

661 tgagtgcccc taaaggttca gaggtggggt aactgcagtt accctcaaac aaaaccgtgg 720

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 780

721 ttttagttgc cctgaaaggt tttacagcat tgttgaggcg gggtaactgc gtttacccgc 780

.......... ....... CMV_immearly_pro ....... ......... CMV_fwd_pri

.......... .......... .......... .......... .......... ..........

781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 840

781 catccgcaca tgccaccctc cagatatatt cgtctcgaga gaccgattga tctcttgggt 840

CMV_fwd_pri ..... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 900

841 gacgaatgac cgaatagctt taattatgct gagtgatatc cctctgggtt cgaccgatcg 900

CMV_immearly_pro .... .......... T7_pro .......... ..........

... AflII(908),HindIII(911) ... .......... .......... ..........

901 gtttaaactt aagcttagcg cagaggcttg gggcagccga gcggcagcca ggccccggcc 960

901 caaatttgaa ttcgaatcgc gtctccgaac cccgtcggct cgccgtcggt ccggggccgg 960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 cgggcctcgg ttccagaagg gagaggagcc cgccaaggcg cgcaagagag cgggctgcct 1020

961 gcccggagcc aaggtcttcc ctctcctcgg gcggttccgc gcgttctctc gcccgacgga 1020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 cgcagtccga gccggagagg gagcgcgagc cgcgccggcc ccggacggcc tccgaaacca 1080

1021 gcgtcaggct cggcctctcc ctcgcgctcg gcgcggccgg ggcctgccgg aggctttggt 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 tggggggttc tcatcatcat catcatcatg gtatggctag catgactggt ggacagcaaa 1140

1081 accccccaag agtagtagta gtagtagtac cataccgatc gtactgacca cctgtcgttt 1140

.......... 6xHis .... Xpress_fwd_pri T7_gene10_leader T7_leader

.......... .......... .... KpnI(1176),BamHI(1179) .... ..........

1141 tgggtcggga tctgtacgac gatgacgata aggtaccagg atccagtgtg gtggaattct 1200

1141 acccagccct agacatgctg ctactgctat tccatggtcc taggtcacac caccttaaga 1200

.......... Xpress_EK .......... .......... .......... ..........

PstI,EcoRV NotI) XhoI(1227) XbaI(1233) ApaI(1243)

1201 gcagatatcc agcacagtgg cggccgctcg agtctagagg gcccgtttaa acccgctgat 1260

1201 cgtctatagg tcgtgtcacc gccggcgagc tcagatctcc cgggcaaatt tgggcgacta 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt 1320

1261 gtcggagctg acacggaaga tcaacggtcg gtagacaaca aacggggagg gggcacggaa 1320

......... BGH_rev_pri ........ bGH_PA_term ......... ..........

.......... .......... .......... .......... .......... ..........

1321 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat 1380

1321 ggaactggga ccttccacgg tgagggtgac aggaaaggat tattttactc ctttaacgta 1380

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg 1440

1381 gcgtaacaga ctcatccaca gtaagataag accccccacc ccaccccgtc ctgtcgttcc 1440

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg 1500

1441 ccctcctaac ccttctgtta tcgtccgtac gacccctacg ccacccgaga taccgaagac 1500

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat 1560

1501 tccgcctttc ttggtcgacc ccgagatccc ccataggggt gcgcgggaca tcgccgcgta 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag 1620

1561 attcgcgccg cccacaccac caatgcgcgt cgcactggcg atgtgaacgg tcgcgggatc 1620

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc 1680

1621 gcgggcgagg aaagcgaaag aagggaagga aagagcggtg caagcggccg aaaggggcag 1680

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 aagctctaaa tcggggcatc cctttagggt tccgatttag tgctttacgg cacctcgacc 1740

1681 ttcgagattt agccccgtag ggaaatccca aggctaaatc acgaaatgcc gtggagctgg 1740

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt 1800

1741 ggttttttga actaatccca ctaccaagtg catcacccgg tagcgggact atctgccaaa 1800

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa 1860

1801 aagcgggaaa ctgcaacctc aggtgcaaga aattatcacc tgagaacaag gtttgacctt 1860

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg gggatttcgg 1920

1861 gttgtgagtt gggatagagc cagataagaa aactaaatat tccctaaaac ccctaaagcc 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa 1980

1921 ggataaccaa ttttttactc gactaaattg tttttaaatt gcgcttaatt aagacacctt 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca ggcaggcaga agtatgcaaa 2040

1981 acacacagtc aatcccacac ctttcagggg tccgaggggt ccgtccgtct tcatacgttt 2040

pBABE_3_pri ........ ...... SV40_enh SV40_pro ...... ..........

.......... .......... .......... .......... .......... ..........

2041 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca 2100

2041 cgtacgtaga gttaatcagt cgttggtcca cacctttcag gggtccgagg ggtcgtccgt 2100

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc 2160

2101 cttcatacgt ttcgtacgta gagttaatca gtcgttggta tcagggcggg gattgaggcg 2160

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt 2220

2161 ggtagggcgg ggattgaggc gggtcaaggc gggtaagagg cggggtaccg actgattaaa 2220

......... SV40_enh SV40_pro SV40_origin ......... ..........

.......... .......... .......... .......... .......... ..........

2221 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag 2280

2221 aaaaataaat acgtctccgg ctccggcgga gacggagact cgataaggtc ttcatcactc 2280

SV40_origin SV40pro_F_pri SV40_pro .... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt 2340

2281 ctccgaaaaa acctccggat ccgaaaacgt ttttcgaggg ccctcgaaca tataggtaaa 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 tcggatctga tcagcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata 2400

2341 agcctagact agtcgtgcac aactgttaat tagtagccgt atcatatagc cgtatcatat 2400

.......... .......... .......... EM7_pro .......... ..........

.......... .......... MscI(2429) .......... .......... ..........

2401 atacgacaag gtgaggaact aaaccatggc caagttgacc agtgccgttc cggtgctcac 2460

2401 tatgctgttc cactccttga tttggtaccg gttcaactgg tcacggcaag gccacgagtg 2460

.......... EM7_pro .......... ......... bleo sh_ble .........

.......... .......... .......... .......... .......... ..........

2461 cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt tctcccggga 2520

2461 gcgcgcgctg cagcggcctc gccagctcaa gacctggctg gccgagccca agagggccct 2520

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc 2580

2521 gaagcacctc ctgctgaagc ggccacacca ggccctgctg cactgggaca agtagtcgcg 2580

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc gcggcctgga 2640

2581 ccaggtcctg gtccaccacg gcctgttgtg ggaccggacc cacacccacg cgccggacct 2640

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg cctccgggcc 2700

2641 gctcgacatg cggctcacca gcctccagca caggtgcttg aaggccctgc ggaggcccgg 2700

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc gcgacccggc 2760

2701 ccggtactgg ctctagccgc tcgtcggcac ccccgccctc aagcgggacg cgctgggccg 2760

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 cggcaactgc gtgcacttcg tggccgagga gcaggactga cacgtgctac gagatttcga 2820

2761 gccgttgacg cacgtgaagc accggctcct cgtcctgact gtgcacgatg ctctaaagct 2820

......... bleo sh_ble ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg 2880

2821 aaggtggcgg cggaagatac tttccaaccc gaagccttag caaaaggccc tgcggccgac 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat 2940

2881 ctactaggag gtcgcgcccc tagagtacga cctcaagaag cgggtggggt tgaacaaata 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt 3000

2941 acgtcgaata ttaccaatgt ttatttcgtt atcgtagtgt ttaaagtgtt tatttcgtaa 3000

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg 3060

3001 aaaaagtgac gtaagatcaa caccaaacag gtttgagtag ttacatagaa tagtacagac 3060

.......... ... SV40_PA_term EBV_rev_pri ... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg 3120

3061 atatggcagc tggagatcga tctcgaaccg cattagtacc agtatcgaca aaggacacac 3120

.......... .......... .......... ....... M13_reverse_pri .......

.......... .......... .......... .......... .......... ..........

3121 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 3180

3121 tttaacaata ggcgagtgtt aaggtgtgtt gtatgctcgg ccttcgtatt tcacatttcg 3180

M13_pUC_rev_pri .... .......... .......... lac_pro ..........

.......... .......... .......... .......... .......... ..........

3181 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 3240

3181 gaccccacgg attactcact cgattgagtg taattaacgc aacgcgagtg acgggcgaaa 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 3300

3241 ggtcagccct ttggacagca cggtcgacgt aattacttag ccggttgcgc gcccctctcc 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt 3360

3301 gccaaacgca taacccgcga gaaggcgaag gagcgagtga ctgagcgacg cgagccagca 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 3420

3361 agccgacgcc gctcgccata gtcgagtgag tttccgccat tatgccaata ggtgtcttag 3420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 3480

3421 tcccctattg cgtcctttct tgtacactcg ttttccggtc gttttccggt ccttggcatt 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 3540

3481 tttccggcgc aacgaccgca aaaaggtatc cgaggcgggg ggactgctcg tagtgttttt 3540

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

3541 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 3600

3541 agctgcgagt tcagtctcca ccgctttggg ctgtcctgat atttctatgg tccgcaaagg 3600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3601 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 3660

3601 gggaccttcg agggagcacg cgagaggaca aggctgggac ggcgaatggc ctatggacag 3660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag 3720

3661 gcggaaagag ggaagccctt cgcaccgcga aagagttacg agtgcgacat ccatagagtc 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 3780

3721 aagccacatc cagcaagcga ggttcgaccc gacacacgtg cttggggggc aagtcgggct 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 3840

3781 ggcgacgcgg aataggccat tgatagcaga actcaggttg ggccattctg tgctgaatag 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 3900

3841 cggtgaccgt cgtcggtgac cattgtccta atcgtctcgc tccatacatc cgccacgatg 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg 3960

3901 tctcaagaac ttcaccaccg gattgatgcc gatgtgatct tcctgtcata aaccatagac 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 4020

3961 gcgagacgac ttcggtcaat ggaagccttt ttctcaacca tcgagaacta ggccgtttgt 4020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 4080

4021 ttggtggcga ccatcgccac caaaaaaaca aacgttcgtc gtctaatgcg cgtctttttt 4080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 4140

4081 tcctagagtt cttctaggaa actagaaaag atgccccaga ctgcgagtca ccttgctttt 4140

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 4200

4141 gagtgcaatt ccctaaaacc agtactctaa tagtttttcc tagaagtgga tctaggaaaa 4200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag 4260

4201 tttaattttt acttcaaaat ttagttagat ttcatatata ctcatttgaa ccagactgtc 4260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat 4320

4261 aatggttacg aattagtcac tccgtggata gagtcgctag acagataaag caagtaggta 4320

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc 4380

4321 tcaacggact gaggggcagc acatctattg atgctatgcc ctcccgaatg gtagaccggg 4380

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa 4440

4381 gtcacgacgt tactatggcg ctctgggtgc gagtggccga ggtctaaata gtcgttattt 4440

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca 4500

4441 ggtcggtcgg ccttcccggc tcgcgtcttc accaggacgt tgaaataggc ggaggtaggt 4500

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... FspI(4556)

4501 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa 4560

4501 cagataatta acaacggccc ttcgatctca ttcatcaagc ggtcaattat caaacgcgtt 4560

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt 4620

4561 gcaacaacgg taacgatgtc cgtagcacca cagtgcgagc agcaaaccat accgaagtaa 4620

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc 4680

4621 gtcgaggcca agggttgcta gttccgctca atgtactagg gggtacaaca cgttttttcg 4680

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact 4740

4681 ccaatcgagg aagccaggag gctagcaaca gtcttcattc aaccggcgtc acaatagtga 4740

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc 4800

4741 gtaccaatac cgtcgtgacg tattaagaga atgacagtac ggtaggcatt ctacgaaaag 4800

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg 4860

4801 acactgacca ctcatgagtt ggttcagtaa gactcttatc acatacgccg ctggctcaac 4860

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct 4920

4861 gagaacgggc cgcagttatg ccctattatg gcgcggtgta tcgtcttgaa attttcacga 4920

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc 4980

4921 gtagtaacct tttgcaagaa gccccgcttt tgagagttcc tagaatggcg acaactctag 4980

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag 5040

4981 gtcaagctac attgggtgag cacgtgggtt gactagaagt cgtagaaaat gaaagtggtc 5040

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac 5100

5041 gcaaagaccc actcgttttt gtccttccgt tttacggcgt tttttccctt attcccgctg 5100

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg 5160

5101 tgcctttaca acttatgagt atgagaagga aaaagttata ataacttcgt aaatagtccc 5160

....... ORF 1 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt 5220

5161 aataacagag tactcgccta tgtataaact tacataaatc tttttatttg tttatcccca 5220

.......... AmpR_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 tccgcgcaca tttccccgaa aagtgccacc tgacgtc... .......... .......... 5257

5221 aggcgcgtgt aaaggggctt ttcacggtgg actgcag... .......... .......... 5257

.......... .......... .......... .......... .......... ..........