Sequence of pcDNA4_His

This sequnce graph is created using BVTech Plasmid, which is DNA sequence analysis and plasmid drawing software for Windows PCs.You can use it to plan your DNA cloning, draw high quality plasmid maps, analyse your DNA sequencing data, align sequences, and much more.

Free to try, $39.98 to buy

Payments will be processed by PayPal. PayPal is the safer, easier way to pay.

Sequence of pcDNA4_His_A

Download the sequence and plasmid map, and then use BVTech Plasmid to view and edit the file.

If you do not have BVTech Plasmid, download the latest version of BVTech Plasmid here.

View the plasmid map on line

Sequence of pcDNA4/His A : 5097 BP; 1190 A; 1312 C; 1302 G; 1293 T; 0 other;

.......... BglII(12) .......... .......... .......... ..........

1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 60

1 ctgcctagcc ctctagaggg ctaggggata ccacgtgaga gtcatgttag acgagactac 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120

61 ggcgtatcaa ttcggtcata gacgagggac gaacacacaa cctccagcga ctcatcacgc 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180

121 gctcgtttta aattcgatgt tgttccgttc cgaactggct gttaacgtac ttcttagacg 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240

181 aatcccaatc cgcaaaacgc gacgaagcgc tacatgcccg gtctatatgc gcaactgtaa 240

.......... .......... .......... .......... .......... ..........

SpeI(249) .......... .......... .......... .......... ..........

241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 300

241 ctaataactg atcaataatt atcattagtt aatgccccag taatcaagta tcgggtatat 300

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 360

301 acctcaaggc gcaatgtatt gaatgccatt taccgggcgg accgactggc gggttgctgg 360

.......... ....... CMV_immearly_pro CAG_enh ....... ..........

.......... .......... .......... .......... .......... ..........

361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 420

361 gggcgggtaa ctgcagttat tactgcatac aagggtatca ttgcggttat ccctgaaagg 420

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 480

421 taactgcagt tacccacctc ataaatgcca tttgacgggt gaaccgtcat gtagttcaca 480

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

NdeI(484) .......... .......... .......... .......... ..........

481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 540

481 tagtatacgg ttcatgcggg ggataactgc agttactgcc atttaccggg cggaccgtaa 540

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 600

541 tacgggtcat gtactggaat accctgaaag gatgaaccgt catgtagatg cataatcagt 600

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 660

601 agcgataatg gtaccactac gccaaaaccg tcatgtagtt acccgcacct atcgccaaac 660

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 720

661 tgagtgcccc taaaggttca gaggtggggt aactgcagtt accctcaaac aaaaccgtgg 720

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 780

721 ttttagttgc cctgaaaggt tttacagcat tgttgaggcg gggtaactgc gtttacccgc 780

.......... ....... CMV_immearly_pro ....... ......... CMV_fwd_pri

.......... .......... .......... .......... .......... ..........

781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 840

781 catccgcaca tgccaccctc cagatatatt cgtctcgaga gaccgattga tctcttgggt 840

CMV_fwd_pri ..... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 900

841 gacgaatgac cgaatagctt taattatgct gagtgatatc cctctgggtt cgaccgatcg 900

CMV_immearly_pro .... .......... T7_pro .......... ..........

... AflII(908),HindIII(911) ... .......... .......... ..........

901 gtttaaactt aagcttacca tggggggttc tcatcatcat catcatcatg gtatggctag 960

901 caaatttgaa ttcgaatggt accccccaag agtagtagta gtagtagtac cataccgatc 960

.......... .......... .......... ........ 6xHis Xpress_fwd_pri

.......... .......... .......... .......... .......... ..........

961 catgactggt ggacagcaaa tgggtcggga tctgtacgac gatgacgata aggtacctag 1020

961 gtactgacca cctgtcgttt acccagccct agacatgctg ctactgctat tccatggatc 1020

Xpress_fwd_pri T7_gene10_leader T7_leader Xpress_EK ..........

EcoRI PstI1044),EcoRV(1047) NotI(1062) XhoI(1068) ....

1021 gatccagtgt ggtggaattc tgcagatatc cagcacagtg gcggccgctc gagtctagag 1080

1021 ctaggtcaca ccaccttaag acgtctatag gtcgtgtcac cgccggcgag ctcagatctc 1080

.......... .......... .......... .......... .......... ..........

ApaI(1084) .......... .......... .......... .......... ..........

1081 ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg 1140

1081 ccgggcaaat ttgggcgact agtcggagct gacacggaag atcaacggtc ggtagacaac 1140

.......... .......... ........ BGH_rev_pri bGH_PA_term ........

.......... .......... .......... .......... .......... ..........

1141 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct 1200

1141 aaacggggag ggggcacgga aggaactggg accttccacg gtgagggtga caggaaagga 1200

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg 1260

1201 ttattttact cctttaacgt agcgtaacag actcatccac agtaagataa gaccccccac 1260

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg 1320

1261 cccaccccgt cctgtcgttc cccctcctaa cccttctgtt atcgtccgta cgacccctac 1320

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc 1380

1321 gccacccgag ataccgaaga ctccgccttt cttggtcgac cccgagatcc cccatagggg 1380

bGH_PA_term ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg 1440

1381 tgcgcgggac atcgccgcgt aattcgcgcc gcccacacca ccaatgcgcg tcgcactggc 1440

.......... .......... .......... f1_origin .......... ..........

.......... .......... .......... .......... .......... ..........

1441 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca 1500

1441 gatgtgaacg gtcgcgggat cgcgggcgag gaaagcgaaa gaagggaagg aaagagcggt 1500

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta 1560

1501 gcaagcggcc gaaaggggca gttcgagatt tagcccccga gggaaatccc aaggctaaat 1560

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc 1620

1561 cacgaaatgc cgtggagctg gggttttttg aactaatccc actaccaagt gcatcacccg 1620

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg 1680

1621 gtagcgggac tatctgccaa aaagcgggaa actgcaacct caggtgcaag aaattatcac 1680

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat 1740

1681 ctgagaacaa ggtttgacct tgttgtgagt tgggatagag ccagataaga aaactaaata 1740

.......... f1_origin .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta 1800

1741 ttccctaaaa cggctaaagc cggataacca attttttact cgactaaatt gtttttaaat 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc 1860

1801 tgcgcttaat taagacacct tacacacagt caatcccaca cctttcaggg gtccgagggg 1860

.......... ......... pBABE_3_pri ..... SV40_enh SV40_pro ......

.......... .......... .......... .......... .......... ..........

1861 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc 1920

1861 tcgtccgtct tcatacgttt cgtacgtaga gttaatcagt cgttggtcca cacctttcag 1920

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat 1980

1921 gggtccgagg ggtcgtccgt cttcatacgt ttcgtacgta gagttaatca gtcgttggta 1980

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc 2040

1981 tcagggcggg gattgaggcg ggtagggcgg ggattgaggc gggtcaaggc gggtaagagg 2040

.......... ...... SV40_enh SV40_pro ..... SV40_origin .........

.......... .......... .......... .......... .......... ..........

2041 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga 2100

2041 cggggtaccg actgattaaa aaaaataaat acgtctccgg ctccggcgga gacggagact 2100

....... SV40_origin SV40_pro SV40pro_F_pri ....... ..........

.......... .......... .......... .......... .......... ..........

2101 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc 2160

2101 cgataaggtc ttcatcactc ctccgaaaaa acctccggat ccgaaaacgt ttttcgaggg 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca 2220

2161 ccctcgaaca tataggtaaa agcctagact agtcgtgcac aactgttaat tagtagccgt 2220

.......... .......... .......... .......... EM7_pro ..........

.......... .......... .......... .......... MscI(2269) ..........

2221 tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc caagttga cc 2280

2221 atcatatagc cgtatcatat tatgctgttc cactccttga tttggtaccg gttcaactgg 2280

.......... .......... EM7_pro .......... ......... bleo sh_ble

.......... .......... .......... .......... .......... ..........

2281 agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac 2340

2281 tcacggcaag gccacgagtg gcgcgcgctg cagcggcctc gccagctcaa gacctggctg 2340

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac 2400

2341 gccgagccca agagggccct gaagcacctc ctgctgaagc ggccacacca ggccctgctg 2400

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg 2460

2401 cactgggaca agtagtcgcg ccaggtcctg gtccaccacg gcctgttgtg ggaccggacc 2460

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac 2520

2461 cacacccacg cgccggacct gctcgacatg cggctcacca gcctccagca caggtgcttg 2520

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag 2580

2521 aaggccctgc ggaggcccgg ccggtactgg ctctagccgc tcgtcggcac ccccgccctc 2580

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga 2640

2581 aagcgggacg cgctgggccg gccgttgacg cacgtgaagc accggctcct cgtcctgact 2640

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc 2700

2641 gtgcacgatg ctctaaagct aaggtggcgg cggaagatac tttccaaccc gaagccttag 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc 2760

2701 caaaaggccc tgcggccgac ctactaggag gtcgcgcccc tagagtacga cctcaagaag 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca 2820

2761 cgggtggggt tgaacaaata acgtcgaata ttaccaatgt ttatttcgtt atcgtagtgt 2820

.......... .......... ......... SV40_PA_term ......... ..........

.......... .......... .......... .......... .......... ..........

2821 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc 2880

2821 ttaaagtgtt tatttcgtaa aaaaagtgac gtaagatcaa caccaaacag gtttgagtag 2880

.......... ......... SV40_PA_term ........ EBV_rev_pri .........

.......... .......... .......... .......... .......... ..........

2881 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg 2940

2881 ttacatagaa tagtacagac atatggcagc tggagatcga tctcgaaccg cattagtacc 2940

SV40_PA_term ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc 3000

2941 agtatcgaca aaggacacac tttaacaata ggcgagtgtt aaggtgtgtt gtatgctcgg 3000

M13_reverse_pri . M13_pUC_rev_pri ....... .......... lac_pro

.......... .......... .......... .......... .......... ..........

3001 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg 3060

3001 ccttcgtatt tcacatttcg gaccccacgg attactcact cgattgagtg taattaacgc 3060

lac_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc 3120

3061 aacgcgagtg acgggcgaaa ggtcagccct ttggacagca cggtcgacgt aattacttag 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact 3180

3121 ccggttgcgc gcccctctcc gccaaacgca taacccgcga gaaggcgaag gagcgagtga 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta 3240

3181 ctgagcgacg cgagccagca agccgacgcc gctcgccata gtcgagtgag tttccgccat 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag 3300

3241 tatgccaata ggtgtcttag tcccctattg cgtcctttct tgtacactcg ttttccggtc 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc 3360

3301 gttttccggt ccttggcatt tttccggcgc aacgaccgca aaaaggtatc cgaggcgggg 3360

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

3361 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta 3420

3361 ggactgctcg tagtgttttt agctgcgagt tcagtctcca ccgctttggg ctgtcctgat 3420

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3421 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg 3480

3421 atttctatgg tccgcaaagg gggaccttcg agggagcacg cgagaggaca aggctgggac 3480

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3481 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc 3540

3481 ggcgaatggc ctatggacag gcggaaagag ggaagccctt cgcaccgcga aagagtatcg 3540

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3541 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac 3600

3541 agtgcgacat ccatagagtc aagccacatc cagcaagcga ggttcgaccc gacacacgtg 3600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3601 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac 3660

3601 cttggggggc aagtcgggct ggcgacgcgg aataggccat tgatagcaga actcaggttg 3660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 3720

3661 ggccattctg tgctgaatag cggtgaccgt cgtcggtgac cattgtccta atcgtctcgc 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga 3780

3721 tccatacatc cgccacgatg tctcaagaac ttcaccaccg gattgatgcc gatgtgatct 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt 3840

3781 tcttgtcata aaccatagac gcgagacgac ttcggtcaat ggaagccttt ttctcaacca 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag 3900

3841 tcgagaacta ggccgtttgt ttggtggcga ccatcgccac caaaaaaaca aacgttcgtc 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct 3960

3901 gtctaatgcg cgtctttttt tcctagagtt cttctaggaa actagaaaag atgccccaga 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg 4020

3961 ctgcgagtca ccttgctttt gagtgcaatt ccctaaaacc agtactctaa tagtttttcc 4020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat 4080

4021 tagaagtgga tctaggaaaa tttaattttt acttcaaaat ttagttagat ttcatatata 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc 4140

4081 ctcatttgaa ccagactgtc aatggttacg aattagtcac tccgtggata gagtcgctag 4140

.......... .......... ....... ORF 3 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

4141 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg 4200

4141 acagataaag caagtaggta tcaacggact gaggggcagc acatctattg atgctatgcc 4200

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct 4260

4201 ctcccgaatg gtagaccggg gtcacgacgt tactatggcg ctctgggtgc gagtggccga 4260

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca 4320

4261 ggtctaaata gtcgttattt ggtcggtcgg ccttcccggc tcgcgtcttc accaggacgt 4320

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg 4380

4321 tgaaataggc ggaggtaggt cagataatta acaacggccc ttcgatctca ttcatcaagc 4380

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... FspI(4396) .......... .......... .......... ..........

4381 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg 4440

4381 ggtcaattat caaacgcgtt gcaacaacgg taacgatgtc cgtagcacca cagtgcgagc 4440

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc 4500

4441 agcaaaccat accgaagtaa gtcgaggcca agggttgcta gttccgctca atgtactagg 4500

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag 4560

4501 gggtacaaca cgttttttcg ccaatcgagg aagccaggag gctagcaaca gtcttcattc 4560

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg 4620

4561 aaccggcgtc acaatagtga gtaccaatac cgtcgtgacg tattaagaga atgacagtac 4620

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag 4680

4621 ggtaggcatt ctacgaaaag acactgacca ctcatgagtt ggttcagtaa gactcttatc 4680

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat 4740

4681 acatacgccg ctggctcaac gagaacgggc cgcagttatg ccctattatg gcgcggtgta 4740

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg 4800

4741 tcgtcttgaa attttcacga gtagtaacct tttgcaagaa gccccgcttt tgagagttcc 4800

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca 4860

4801 tagaatggcg acaactctag gtcaagctac attgggtgag cacgtgggtt gactagaagt 4860

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca 4920

4861 cgtagaaaat gaaagtggtc gcaaagaccc actcgttttt gtccttccgt tttacggcgt 4920

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat 4980

4921 tttttccctt attcccgctg tgcctttaca acttatgagt atgagaagga aaaagttata 4980

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag 5040

4981 ataacttcgt aaatagtccc aataacagag tactcgccta tgtataaact tacataaatc 5040

.......... .......... .......... AmpR_pro .......... ..........

.......... .......... .......... .......... .......... ..........

5041 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc... 5097

5041 tttttatttg tttatcccca aggcgcgtgt aaaggggctt ttcacggtgg actgcag... 5097

.......... .......... .......... .......... .......... ..........