Sequence of pcDNA4_His

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Sequence of pcDNA4_His_B

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Sequence of pcDNA4/His B : 5098 BP; 1191 A; 1312 C; 1302 G; 1293 T; 0 other;

.......... BglII(12) .......... .......... .......... ..........

1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 60

1 ctgcctagcc ctctagaggg ctaggggata ccacgtgaga gtcatgttag acgagactac 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120

61 ggcgtatcaa ttcggtcata gacgagggac gaacacacaa cctccagcga ctcatcacgc 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180

121 gctcgtttta aattcgatgt tgttccgttc cgaactggct gttaacgtac ttcttagacg 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240

181 aatcccaatc cgcaaaacgc gacgaagcgc tacatgcccg gtctatatgc gcaactgtaa 240

.......... .......... .......... .......... .......... ..........

SpeI(249) .......... .......... .......... .......... ..........

241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 300

241 ctaataactg atcaataatt atcattagtt aatgccccag taatcaagta tcgggtatat 300

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 360

301 acctcaaggc gcaatgtatt gaatgccatt taccgggcgg accgactggc gggttgctgg 360

.......... ....... CMV_immearly_pro CAG_enh ....... ..........

.......... .......... .......... .......... .......... ..........

361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 420

361 gggcgggtaa ctgcagttat tactgcatac aagggtatca ttgcggttat ccctgaaagg 420

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 480

421 taactgcagt tacccacctc ataaatgcca tttgacgggt gaaccgtcat gtagttcaca 480

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

NdeI(484) .......... .......... .......... .......... ..........

481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 540

481 tagtatacgg ttcatgcggg ggataactgc agttactgcc atttaccggg cggaccgtaa 540

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 600

541 tacgggtcat gtactggaat accctgaaag gatgaaccgt catgtagatg cataatcagt 600

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 660

601 agcgataatg gtaccactac gccaaaaccg tcatgtagtt acccgcacct atcgccaaac 660

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 720

661 tgagtgcccc taaaggttca gaggtggggt aactgcagtt accctcaaac aaaaccgtgg 720

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 780

721 ttttagttgc cctgaaaggt tttacagcat tgttgaggcg gggtaactgc gtttacccgc 780

.......... ....... CMV_immearly_pro ....... ......... CMV_fwd_pri

.......... .......... .......... .......... .......... ..........

781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 840

781 catccgcaca tgccaccctc cagatatatt cgtctcgaga gaccgattga tctcttgggt 840

CMV_fwd_pri ..... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 900

841 gacgaatgac cgaatagctt taattatgct gagtgatatc cctctgggtt cgaccgatcg 900

CMV_immearly_pro .... .......... T7_pro .......... ..........

... AflII(908),HindIII(911) ... .......... .......... ..........

901 gtttaaactt aagcttacca tggggggttc tcatcatcat catcatcatg gtatggctag 960

901 caaatttgaa ttcgaatggt accccccaag agtagtagta gtagtagtac cataccgatc 960

.......... .......... .......... ........ 6xHis Xpress_fwd_pri

.......... .......... .......... .......... .......... ..........

961 catgactggt ggacagcaaa tgggtcggga tctgtacgac gatgacgata aggtacctaa 1020

961 gtactgacca cctgtcgttt acccagccct agacatgctg ctactgctat tccatggatt 1020

Xpress_fwd_pri T7_gene10_leader T7_leader Xpress_EK ..........

BamHI EcoRI PstI,EcoRV1048) NotI(1063) XhoI(1069)

1021 ggatccagtg tggtggaatt ctgcagatat ccagcacagt ggcggccgct cgagtctaga 1080

1021 cctaggtcac accaccttaa gacgtctata ggtcgtgtca ccgccggcga gctcagatct 1080

.......... .......... .......... .......... .......... ..........

ApaI(1085) .......... .......... .......... .......... ..........

1081 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt 1140

1081 cccgggcaaa tttgggcgac tagtcggagc tgacacggaa gatcaacggt cggtagacaa 1140

.......... .......... ........ BGH_rev_pri bGH_PA_term ........

.......... .......... .......... .......... .......... ..........

1141 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc 1200

1141 caaacgggga gggggcacgg aaggaactgg gaccttccac ggtgagggtg acaggaaagg 1200

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt 1260

1201 attattttac tcctttaacg tagcgtaaca gactcatcca cagtaagata agacccccca 1260

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat 1320

1261 ccccaccccg tcctgtcgtt ccccctccta acccttctgt tatcgtccgt acgaccccta 1320

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc 1380

1321 cgccacccga gataccgaag actccgcctt tcttggtcga ccccgagatc ccccataggg 1380

bGH_PA_term ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc 1440

1381 gtgcgcggga catcgccgcg taattcgcgc cgcccacacc accaatgcgc gtcgcactgg 1440

.......... .......... .......... f1_origin .......... ..........

.......... .......... .......... .......... .......... ..........

1441 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc 1500

1441 cgatgtgaac ggtcgcggga tcgcgggcga ggaaagcgaa agaagggaag gaaagagcgg 1500

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt 1560

1501 tgcaagcggc cgaaaggggc agttcgagat ttagcccccg agggaaatcc caaggctaaa 1560

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg 1620

1561 tcacgaaatg ccgtggagct ggggtttttt gaactaatcc cactaccaag tgcatcaccc 1620

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt 1680

1621 ggtagcggga ctatctgcca aaaagcggga aactgcaacc tcaggtgcaa gaaattatca 1680

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta 1740

1681 cctgagaaca aggtttgacc ttgttgtgag ttgggataga gccagataag aaaactaaat 1740

.......... f1_origin .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt 1800

1741 attccctaaa acggctaaag ccggataacc aattttttac tcgactaaat tgtttttaaa 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc 1860

1801 ttgcgcttaa ttaagacacc ttacacacag tcaatcccac acctttcagg ggtccgaggg 1860

.......... ......... pBABE_3_pri ..... SV40_enh SV40_pro ......

.......... .......... .......... .......... .......... ..........

1861 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt 1920

1861 gtcgtccgtc ttcatacgtt tcgtacgtag agttaatcag tcgttggtcc acacctttca 1920

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca 1980

1921 ggggtccgag gggtcgtccg tcttcatacg tttcgtacgt agagttaatc agtcgttggt 1980

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc 2040

1981 atcagggcgg ggattgaggc gggtagggcg gggattgagg cgggtcaagg cgggtaagag 2040

.......... ...... SV40_enh SV40_pro ..... SV40_origin .........

.......... .......... .......... .......... .......... ..........

2041 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg 2100

2041 gcggggtacc gactgattaa aaaaaataaa tacgtctccg gctccggcgg agacggagac 2100

.......... ... SV40_pro SV40_origin SV40pro_F_pri ... ..........

.......... .......... .......... .......... .......... ..........

2101 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc 2160

2101 tcgataaggt cttcatcact cctccgaaaa aacctccgga tccgaaaacg tttttcgagg 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc 2220

2161 gccctcgaac atataggtaa aagcctagac tagtcgtgca caactgttaa ttagtagccg 2220

.......... .......... .......... .......... EM7_pro ..........

.......... .......... .......... .......... MscI(2270) ..........

2221 atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagttg ac 2280

2221 tatcatatag ccgtatcata ttatgctgtt ccactccttg atttggtacc ggttcaactg 2280

.......... .......... EM7_pro .......... ......... bleo sh_ble

.......... .......... .......... .......... .......... ..........

2281 cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga 2340

2281 gtcacggcaa ggccacgagt ggcgcgcgct gcagcggcct cgccagctca agacctggct 2340

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg tccgggacga 2400

2341 ggccgagccc aagagggccc tgaagcacct cctgctgaag cggccacacc aggccctgct 2400

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca ccctggcctg 2460

2401 gcactgggac aagtagtcgc gccaggtcct ggtccaccac ggcctgttgt gggaccggac 2460

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa 2520

2461 ccacacccac gcgccggacc tgctcgacat gcggctcacc agcctccagc acaggtgctt 2520

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt gggggcggga 2580

2521 gaaggccctg cggaggcccg gccggtactg gctctagccg ctcgtcggca cccccgccct 2580

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg agcaggactg 2640

2581 caagcgggac gcgctgggcc ggccgttgac gcacgtgaag caccggctcc tcgtcctgac 2640

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat 2700

2641 tgtgcacgat gctctaaagc taaggtggcg gcggaagata ctttccaacc cgaagcctta 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt 2760

2701 gcaaaaggcc ctgcggccga cctactagga ggtcgcgccc ctagagtacg acctcaagaa 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac 2820

2761 gcgggtgggg ttgaacaaat aacgtcgaat attaccaatg tttatttcgt tatcgtagtg 2820

.......... .......... ......... SV40_PA_term ......... ..........

.......... .......... .......... .......... .......... ..........

2821 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat 2880

2821 tttaaagtgt ttatttcgta aaaaaagtga cgtaagatca acaccaaaca ggtttgagta 2880

.......... ......... SV40_PA_term ......... ......... EBV_rev_pri

.......... .......... .......... .......... .......... ..........

2881 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg 2940

2881 gttacataga atagtacaga catatggcag ctggagatcg atctcgaacc gcattagtac 2940

SV40_PA_term ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc 3000

2941 cagtatcgac aaaggacaca ctttaacaat aggcgagtgt taaggtgtgt tgtatgctcg 3000

M13_reverse_pri . M13_pUC_rev_pri ....... .......... lac_pro

.......... .......... .......... .......... .......... ..........

3001 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc 3060

3001 gccttcgtat ttcacatttc ggaccccacg gattactcac tcgattgagt gtaattaacg 3060

lac_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat 3120

3061 caacgcgagt gacgggcgaa aggtcagccc tttggacagc acggtcgacg taattactta 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac 3180

3121 gccggttgcg cgcccctctc cgccaaacgc ataacccgcg agaaggcgaa ggagcgagtg 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 3240

3181 actgagcgac gcgagccagc aagccgacgc cgctcgccat agtcgagtga gtttccgcca 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 3300

3241 ttatgccaat aggtgtctta gtcccctatt gcgtcctttc ttgtacactc gttttccggt 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 3360

3301 cgttttccgg tccttggcat ttttccggcg caacgaccgc aaaaaggtat ccgaggcggg 3360

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

3361 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 3420

3361 gggactgctc gtagtgtttt tagctgcgag ttcagtctcc accgctttgg gctgtcctga 3420

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3421 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 3480

3421 tatttctatg gtccgcaaag ggggaccttc gagggagcac gcgagaggac aaggctggga 3480

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3481 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 3540

3481 cggcgaatgg cctatggaca ggcggaaaga gggaagccct tcgcaccgcg aaagagtatc 3540

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3541 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 3600

3541 gagtgcgaca tccatagagt caagccacat ccagcaagcg aggttcgacc cgacacacgt 3600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3601 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 3660

3601 gcttgggggg caagtcgggc tggcgacgcg gaataggcca ttgatagcag aactcaggtt 3660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 3720

3661 gggccattct gtgctgaata gcggtgaccg tcgtcggtga ccattgtcct aatcgtctcg 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 3780

3721 ctccatacat ccgccacgat gtctcaagaa cttcaccacc ggattgatgc cgatgtgatc 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 3840

3781 ttcttgtcat aaaccataga cgcgagacga cttcggtcaa tggaagcctt tttctcaacc 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 3900

3841 atcgagaact aggccgtttg tttggtggcg accatcgcca ccaaaaaaac aaacgttcgt 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 3960

3901 cgtctaatgc gcgtcttttt ttcctagagt tcttctagga aactagaaaa gatgccccag 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 4020

3961 actgcgagtc accttgcttt tgagtgcaat tccctaaaac cagtactcta atagtttttc 4020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 4080

4021 ctagaagtgg atctaggaaa atttaatttt tacttcaaaa tttagttaga tttcatatat 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat 4140

4081 actcatttga accagactgt caatggttac gaattagtca ctccgtggat agagtcgcta 4140

.......... .......... .......... ....... ORF 1 Ampicillin .......

.......... .......... .......... .......... .......... ..........

4141 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 4200

4141 gacagataaa gcaagtaggt atcaacggac tgaggggcag cacatctatt gatgctatgc 4200

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 4260

4201 cctcccgaat ggtagaccgg ggtcacgacg ttactatggc gctctgggtg cgagtggccg 4260

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 4320

4261 aggtctaaat agtcgttatt tggtcggtcg gccttcccgg ctcgcgtctt caccaggacg 4320

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 4380

4321 ttgaaatagg cggaggtagg tcagataatt aacaacggcc cttcgatctc attcatcaag 4380

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... FspI(4397) .......... .......... .......... ..........

4381 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc 4440

4381 cggtcaatta tcaaacgcgt tgcaacaacg gtaacgatgt ccgtagcacc acagtgcgag 4440

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 4500

4441 cagcaaacca taccgaagta agtcgaggcc aagggttgct agttccgctc aatgtactag 4500

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 4560

4501 ggggtacaac acgttttttc gccaatcgag gaagccagga ggctagcaac agtcttcatt 4560

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 4620

4561 caaccggcgt cacaatagtg agtaccaata ccgtcgtgac gtattaagag aatgacagta 4620

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 4680

4621 cggtaggcat tctacgaaaa gacactgacc actcatgagt tggttcagta agactcttat 4680

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca 4740

4681 cacatacgcc gctggctcaa cgagaacggg ccgcagttat gccctattat ggcgcggtgt 4740

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 4800

4741 atcgtcttga aattttcacg agtagtaacc ttttgcaaga agccccgctt ttgagagttc 4800

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 4860

4801 ctagaatggc gacaactcta ggtcaagcta cattgggtga gcacgtgggt tgactagaag 4860

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 4920

4861 tcgtagaaaa tgaaagtggt cgcaaagacc cactcgtttt tgtccttccg ttttacggcg 4920

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 4980

4921 ttttttccct tattcccgct gtgcctttac aacttatgag tatgagaagg aaaaagttat 4980

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 5040

4981 aataacttcg taaatagtcc caataacaga gtactcgcct atgtataaac ttacataaat 5040

.......... .......... .......... AmpR_pro .......... ..........

.......... .......... .......... .......... .......... ..........

5041 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtc.. 5098

5041 ctttttattt gtttatcccc aaggcgcgtg taaaggggct tttcacggtg gactgcag.. 5098

.......... .......... .......... .......... .......... ..........