Sequence of pcDNA4_V5-His

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Sequence of pcDNA4_V5-His_A

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Sequence of pcDNA4/V5-His A : 5092 BP; 1184 A; 1326 C; 1289 G; 1293 T; 0 other;

.......... BglII(12) .......... .......... .......... ..........

1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 60

1 ctgcctagcc ctctagaggg ctaggggata ccacgtgaga gtcatgttag acgagactac 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120

61 ggcgtatcaa ttcggtcata gacgagggac gaacacacaa cctccagcga ctcatcacgc 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180

121 gctcgtttta aattcgatgt tgttccgttc cgaactggct gttaacgtac ttcttagacg 180

.......... .......... .......... .......... .......... ..........

.......... .......... NruI(208) .......... .......... ..........

181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240

181 aatcccaatc cgcaaaacgc gacgaagcgc tacatgcccg gtctatatgc gcaactgtaa 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 300

241 ctaataactg atcaataatt atcattagtt aatgccccag taatcaagta tcgggtatat 300

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 360

301 acctcaaggc gcaatgtatt gaatgccatt taccgggcgg accgactggc gggttgctgg 360

.......... ....... CMV_immearly_pro CAG_enh ....... ..........

.......... .......... .......... .......... .......... ..........

361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 420

361 gggcgggtaa ctgcagttat tactgcatac aagggtatca ttgcggttat ccctgaaagg 420

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 480

421 taactgcagt tacccacctc ataaatgcca tttgacgggt gaaccgtcat gtagttcaca 480

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

NdeI(484) .......... .......... .......... .......... ..........

481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 540

481 tagtatacgg ttcatgcggg ggataactgc agttactgcc atttaccggg cggaccgtaa 540

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 600

541 tacgggtcat gtactggaat accctgaaag gatgaaccgt catgtagatg cataatcagt 600

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 660

601 agcgataatg gtaccactac gccaaaaccg tcatgtagtt acccgcacct atcgccaaac 660

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 720

661 tgagtgcccc taaaggttca gaggtggggt aactgcagtt accctcaaac aaaaccgtgg 720

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 780

721 ttttagttgc cctgaaaggt tttacagcat tgttgaggcg gggtaactgc gtttacccgc 780

.......... ....... CMV_immearly_pro ....... ......... CMV_fwd_pri

.......... .......... .......... .......... .......... ..........

781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 840

781 catccgcaca tgccaccctc cagatatatt cgtctcgaga gaccgattga tctcttgggt 840

CMV_fwd_pri ..... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 900

841 gacgaatgac cgaatagctt taattatgct gagtgatatc cctctgggtt cgaccgatcg 900

CMV_immearly_pro .... .......... T7_pro .......... ..........

AflII(908),HindIII(911) KpnI(921) BamHI(929) ...... ..........

901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattctgc 960

901 caaatttgaa ttcgaaccat ggctcgagcc taggtgatca ggtcacacca ccttaagacg 960

.......... .......... .......... .......... .......... ..........

PstI,EcoRV NotI XhoI XbaI) ApaI(1001),BstBI(1004)

961 agatatccag cacagtggcg gccgctcgag tctagagggc ccttcgaagg taagcctatc 1020

961 tctataggtc gtgtcaccgc cggcgagctc agatctcccg ggaagcttcc attcggatag 1020

.......... .......... .......... .......... .......... V5

.......... .......... .......... AgeI(1054) .......... ..........

1021 cctaaccctc tcctcggtct cgattctacg cgtaccggtc atcatcacca tcaccattga 1080

1021 ggattgggag aggagccaga gctaagatgc gcatggccag tagtagtggt agtggtaact 1080

.......... V5 .......... .......... 6xHis ..........

.......... .......... .......... .......... .......... ..........

1081 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc 1140

1081 caaatttggg cgactagtcg gagctgacac ggaagatcaa cggtcggtag acaacaaacg 1140

.......... ......... BGH_rev_pri ........ bGH_PA_term .........

.......... .......... .......... .......... .......... ..........

1141 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa 1200

1141 gggagggggc acggaaggaa ctgggacctt ccacggtgag ggtgacagga aaggattatt 1200

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg 1260

1201 ttactccttt aacgtagcgt aacagactca tccacagtaa gataagaccc cccaccccac 1260

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg 1320

1261 cccgtcctgt cgttccccct cctaaccctt ctgttatcgt ccgtacgacc cctacgccac 1320

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg 1380

1321 ccgagatacc gaagactccg cctttcttgg tcgaccccga gatcccccat aggggtgcgc 1380

bGH_PA_term ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca 1440

1381 gggacatcgc cgcgtaattc gcgccgccca caccaccaat gcgcgtcgca ctggcgatgt 1440

.......... .......... .......... f1_origin .......... ..........

.......... .......... .......... .......... .......... ..........

1441 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc 1500

1441 gaacggtcgc gggatcgcgg gcgaggaaag cgaaagaagg gaaggaaaga gcggtgcaag 1500

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct 1560

1501 cggccgaaag gggcagttcg agatttagcc cccgagggaa atcccaaggc taaatcacga 1560

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg 1620

1561 aatgccgtgg agctggggtt ttttgaacta atcccactac caagtgcatc acccggtagc 1620

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc 1680

1621 gggactatct gccaaaaagc gggaaactgc aacctcaggt gcaagaaatt atcacctgag 1680

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg 1740

1681 aacaaggttt gaccttgttg tgagttggga tagagccaga taagaaaact aaatattccc 1740

f1_origin .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg 1800

1741 taaaacggct aaagccggat aaccaatttt ttactcgact aaattgtttt taaattgcgc 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag 1860

1801 ttaattaaga caccttacac acagtcaatc ccacaccttt caggggtccg aggggtcgtc 1860

.......... ......... pBABE_3_pri SV40_enh SV40_pro .........

.......... .......... .......... .......... .......... ..........

1861 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag 1920

1861 cgtcttcata cgtttcgtac gtagagttaa tcagtcgttg gtccacacct ttcaggggtc 1920

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc 1980

1921 cgaggggtcg tccgtcttca tacgtttcgt acgtagagtt aatcagtcgt tggtatcagg 1980

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc 2040

1981 gcggggattg aggcgggtag ggcggggatt gaggcgggtc aaggcgggta agaggcgggg 2040

.......... ..... SV40_enh SV40_pro SV40_origin ..... ..........

.......... .......... .......... .......... .......... ..........

2041 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat 2100

2041 taccgactga ttaaaaaaaa taaatacgtc tccggctccg gcggagacgg agactcgata 2100

... SV40_origin SV40_pro SV40pro_F_pri ... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag 2160

2101 aggtcttcat cactcctccg aaaaaacctc cggatccgaa aacgtttttc gagggccctc 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta 2220

2161 gaacatatag gtaaaagcct agactagtcg tgcacaactg ttaattagta gccgtatcat 2220

.......... .......... .......... .......... EM7_pro ..........

.......... .......... .......... .......... MscI(2264) ..........

2221 tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc 2280

2221 atagccgtat catattatgc tgttccactc cttgatttgg taccggttca actggtcacg 2280

.......... EM7_pro .......... ......... bleo sh_ble .........

.......... .......... .......... .......... .......... ..........

2281 cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct 2340

2281 gcaaggccac gagtggcgcg cgctgcagcg gcctcgccag ctcaagacct ggctggccga 2340

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac 2400

2341 gcccaagagg gccctgaagc acctcctgct gaagcggcca caccaggccc tgctgcactg 2400

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg 2460

2401 ggacaagtag tcgcgccagg tcctggtcca ccacggcctg ttgtgggacc ggacccacac 2460

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg 2520

2461 ccacgcgccg gacctgctcg acatgcggct caccagcctc cagcacaggt gcttgaaggc 2520

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc 2580

2521 cctgcggagg cccggccggt actggctcta gccgctcgtc ggcacccccg ccctcaagcg 2580

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt 2640

2581 ggacgcgctg ggccggccgt tgacgcacgt gaagcaccgg ctcctcgtcc tgactgtgca 2640

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt 2700

2641 cgatgctcta aagctaaggt ggcggcggaa gatactttcc aacccgaagc cttagcaaaa 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca 2760

2701 ggccctgcgg ccgacctact aggaggtcgc gcccctagag tacgacctca agaagcgggt 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt 2820

2761 ggggttgaac aaataacgtc gaatattacc aatgtttatt tcgttatcgt agtgtttaaa 2820

.......... .......... ......... SV40_PA_term ......... ..........

.......... .......... .......... .......... .......... ..........

2821 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac t catcaatgt 2880

2821 gtgtttattt cgtaaaaaaa gtgacgtaag atcaacacca aacaggtttg agtagttaca 2880

.......... ......... SV40_PA_term ........ EBV_rev_pri .........

.......... .......... .......... .......... .......... ..........

2881 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata 2940

2881 tagaatagta cagacatatg gcagctggag atcgatctcg aaccgcatta gtaccagtat 2940

.......... .......... .......... .......... ....... M13_reverse_pri

.......... .......... .......... .......... .......... ..........

2941 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag 3000

2941 cgacaaagga cacactttaa caataggcga gtgttaaggt gtgttgtatg ctcggccttc 3000

M13_reverse_pri M13_pUC_rev_pri ........ .......... lac_pro

.......... .......... .......... .......... .......... ..........

3001 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg 3060

3001 gtatttcaca tttcggaccc cacggattac tcactcgatt gagtgtaatt aacgcaacgc 3060

lac_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca 3120

3061 gagtgacggg cgaaaggtca gccctttgga cagcacggtc gacgtaatta cttagccggt 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc 3180

3121 tgcgcgcccc tctccgccaa acgcataacc cgcgagaagg cgaaggagcg agtgactgag 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 3240

3181 cgacgcgagc cagcaagccg acgccgctcg ccatagtcga gtgagtttcc gccattatgc 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 3300

3241 caataggtgt cttagtcccc tattgcgtcc tttcttgtac actcgttttc cggtcgtttt 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 3360

3301 ccggtccttg gcatttttcc ggcgcaacga ccgcaaaaag gtatccgagg cggggggact 3360

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

3361 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 3420

3361 gctcgtagtg tttttagctg cgagttcagt ctccaccgct ttgggctgtc ctgatatttc 3420

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3421 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 3480

3421 tatggtccgc aaagggggac cttcgaggga gcacgcgaga ggacaaggct gggacggcga 3480

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3481 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 3540

3481 atggcctatg gacaggcgga aagagggaag cccttcgcac cgcgaaagag tatcgagtgc 3540

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3541 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 3600

3541 gacatccata gagtcaagcc acatccagca agcgaggttc gacccgacac acgtgcttgg 3600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3601 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 3660

3601 ggggcaagtc gggctggcga cgcggaatag gccattgata gcagaactca ggttgggcca 3660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 3720

3661 ttctgtgctg aatagcggtg accgtcgtcg gtgaccattg tcctaatcgt ctcgctccat 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac 3780

3721 acatccgcca cgatgtctca agaacttcac caccggattg atgccgatgt gatcttcttg 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 3840

3781 tcataaacca tagacgcgag acgacttcgg tcaatggaag cctttttctc aaccatcgag 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 3900

3841 aactaggccg tttgtttggt ggcgaccatc gccaccaaaa aaacaaacgt tcgtcgtcta 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 3960

3901 atgcgcgtct ttttttccta gagttcttct aggaaactag aaaagatgcc ccagactgcg 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 4020

3961 agtcaccttg cttttgagtg caattcccta aaaccagtac tctaatagtt tttcctagaa 4020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 4080

4021 gtggatctag gaaaatttaa tttttacttc aaaatttagt tagatttcat atatactcat 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 4140

4081 ttgaaccaga ctgtcaatgg ttacgaatta gtcactccgt ggatagagtc gctagacaga 4140

.......... .......... ....... ORF 1 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

4141 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 4200

4141 taaagcaagt aggtatcaac ggactgaggg gcagcacatc tattgatgct atgccctccc 4200

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 4260

4201 gaatggtaga ccggggtcac gacgttacta tggcgctctg ggtgcgagtg gccgaggtct 4260

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 4320

4261 aaatagtcgt tatttggtcg gtcggccttc ccggctcgcg tcttcaccag gacgttgaaa 4320

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 4380

4321 taggcggagg taggtcagat aattaacaac ggcccttcga tctcattcat caagcggtca 4380

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... FspI(4391) .......... .......... .......... ..........

4381 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt 4440

4381 attatcaaac gcgttgcaac aacggtaacg atgtccgtag caccacagtg cgagcagcaa 4440

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 4500

4441 accataccga agtaagtcga ggccaagggt tgctagttcc gctcaatgta ctagggggta 4500

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 4560

4501 caacacgttt tttcgccaat cgaggaagcc aggaggctag caacagtctt cattcaaccg 4560

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 4620

4561 gcgtcacaat agtgagtacc aataccgtcg tgacgtatta agagaatgac agtacggtag 4620

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 4680

4621 gcattctacg aaaagacact gaccactcat gagttggttc agtaagactc ttatcacata 4680

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag 4740

4681 cgccgctggc tcaacgagaa cgggccgcag ttatgcccta ttatggcgcg gtgtatcgtc 4740

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 4800

4741 ttgaaatttt cacgagtagt aaccttttgc aagaagcccc gcttttgaga gttcctagaa 4800

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 4860

4801 tggcgacaac tctaggtcaa gctacattgg gtgagcacgt gggttgacta gaagtcgtag 4860

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 4920

4861 aaaatgaaag tggtcgcaaa gacccactcg tttttgtcct tccgttttac ggcgtttttt 4920

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 4980

4921 cccttattcc cgctgtgcct ttacaactta tgagtatgag aaggaaaaag ttataataac 4980

....... ORF 1 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 5040

4981 ttcgtaaata gtcccaataa cagagtactc gcctatgtat aaacttacat aaatcttttt 5040

.......... .......... .......... AmpR_pro .......... ..........

.......... .......... .......... .......... .......... ..........

5041 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tc........ 5092

5041 atttgtttat ccccaaggcg cgtgtaaagg ggcttttcac ggtggactgc ag........ 5092

.......... .......... .......... .......... .......... ..........