Sequence of pcDNA4_V5-His

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Sequence of pcDNA4_V5-His_B

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Sequence of pcDNA4/V5-His B : 5096 BP; 1184 A; 1327 C; 1292 G; 1293 T; 0 other;

.......... BglII(12) .......... .......... .......... ..........

1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 60

1 ctgcctagcc ctctagaggg ctaggggata ccacgtgaga gtcatgttag acgagactac 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120

61 ggcgtatcaa ttcggtcata gacgagggac gaacacacaa cctccagcga ctcatcacgc 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180

121 gctcgtttta aattcgatgt tgttccgttc cgaactggct gttaacgtac ttcttagacg 180

.......... .......... .......... .......... .......... ..........

.......... .......... NruI(208) .......... .......... ..........

181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240

181 aatcccaatc cgcaaaacgc gacgaagcgc tacatgcccg gtctatatgc gcaactgtaa 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 300

241 ctaataactg atcaataatt atcattagtt aatgccccag taatcaagta tcgggtatat 300

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 360

301 acctcaaggc gcaatgtatt gaatgccatt taccgggcgg accgactggc gggttgctgg 360

.......... ....... CMV_immearly_pro CAG_enh ....... ..........

.......... .......... .......... .......... .......... ..........

361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 420

361 gggcgggtaa ctgcagttat tactgcatac aagggtatca ttgcggttat ccctgaaagg 420

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 480

421 taactgcagt tacccacctc ataaatgcca tttgacgggt gaaccgtcat gtagttcaca 480

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

NdeI(484) .......... .......... .......... .......... ..........

481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 540

481 tagtatacgg ttcatgcggg ggataactgc agttactgcc atttaccggg cggaccgtaa 540

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 600

541 tacgggtcat gtactggaat accctgaaag gatgaaccgt catgtagatg cataatcagt 600

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 660

601 agcgataatg gtaccactac gccaaaaccg tcatgtagtt acccgcacct atcgccaaac 660

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 720

661 tgagtgcccc taaaggttca gaggtggggt aactgcagtt accctcaaac aaaaccgtgg 720

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 780

721 ttttagttgc cctgaaaggt tttacagcat tgttgaggcg gggtaactgc gtttacccgc 780

.......... ....... CMV_immearly_pro ....... ......... CMV_fwd_pri

.......... .......... .......... .......... .......... ..........

781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 840

781 catccgcaca tgccaccctc cagatatatt cgtctcgaga gaccgattga tctcttgggt 840

CMV_fwd_pri ..... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 900

841 gacgaatgac cgaatagctt taattatgct gagtgatatc cctctgggtt cgaccgatcg 900

CMV_immearly_pro .... .......... T7_pro .......... ..........

AflII(908),HindIII(911) KpnI(921) BamHI(929) ...... ..........

901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattctgc 960

901 caaatttgaa ttcgaaccat ggctcgagcc taggtgatca ggtcacacca ccttaagacg 960

.......... .......... .......... .......... .......... ..........

PstI,EcoRV NotI XhoI XbaI ApaI,SacII),BstBI(1008)

961 agatatccag cacagtggcg gccgctcgag tctagagggc ccgcggttcg aaggtaagcc 1020

961 tctataggtc gtgtcaccgc cggcgagctc agatctcccg ggcgccaagc ttccattcgg 1020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... AgeI(1058) .......... ..........

1021 tatccctaac cctctcctcg gtctcgattc tacgcgtacc ggtcatcatc accatcacca 1080

1021 atagggattg ggagaggagc cagagctaag atgcgcatgg ccagtagtag tggtagtggt 1080

.......... V5 .......... .......... .......... 6xHis

.......... .......... .......... .......... .......... ..........

1081 ttgagtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt 1140

1081 aactcaaatt tgggcgacta gtcggagctg acacggaaga tcaacggtcg gtagacaaca 1140

.......... .......... ........ BGH_rev_pri bGH_PA_term ........

.......... .......... .......... .......... .......... ..........

1141 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta 1200

1141 aacggggagg gggcacggaa ggaactggga ccttccacgg tgagggtgac aggaaaggat 1200

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg 1260

1201 tattttactc ctttaacgta gcgtaacaga ctcatccaca gtaagataag accccccacc 1260

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc 1320

1261 ccaccccgtc ctgtcgttcc ccctcctaac ccttctgtta tcgtccgtac gacccctacg 1320

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca 1380

1321 ccacccgaga taccgaagac tccgcctttc ttggtcgacc ccgagatccc ccataggggt 1380

bGH_PA_term ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc 1440

1381 gcgcgggaca tcgccgcgta attcgcgccg cccacaccac caatgcgcgt cgcactggcg 1440

.......... .......... .......... f1_origin .......... ..........

.......... .......... .......... .......... .......... ..........

1441 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac 1500

1441 atgtgaacgg tcgcgggatc gcgggcgagg aaagcgaaag aagggaagga aagagcggtg 1500

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag 1560

1501 caagcggccg aaaggggcag ttcgagattt agcccccgag ggaaatccca aggctaaatc 1560

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc 1620

1561 acgaaatgcc gtggagctgg ggttttttga actaatccca ctaccaagtg catcacccgg 1620

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg 1680

1621 tagcgggact atctgccaaa aagcgggaaa ctgcaacctc aggtgcaaga aattatcacc 1680

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata 1740

1681 tgagaacaag gtttgacctt gttgtgagtt gggatagagc cagataagaa aactaaatat 1740

.......... f1_origin .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa 1800

1741 tccctaaaac ggctaaagcc ggataaccaa ttttttactc gactaaattg tttttaaatt 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca 1860

1801 gcgcttaatt aagacacctt acacacagtc aatcccacac ctttcagggg tccgaggggt 1860

.......... ......... pBABE_3_pri ..... SV40_enh SV40_pro ......

.......... .......... .......... .......... .......... ..........

1861 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc 1920

1861 cgtccgtctt catacgtttc gtacgtagag ttaatcagtc gttggtccac acctttcagg 1920

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata 1980

1921 ggtccgaggg gtcgtccgtc ttcatacgtt tcgtacgtag agttaatcag tcgttggtat 1980

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg 2040

1981 cagggcgggg attgaggcgg gtagggcggg gattgaggcg ggtcaaggcg ggtaagaggc 2040

.......... ...... SV40_enh SV40_pro ..... SV40_origin .........

.......... .......... .......... .......... .......... ..........

2041 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag 2100

2041 ggggtaccga ctgattaaaa aaaataaata cgtctccggc tccggcggag acggagactc 2100

....... SV40_origin SV40_pro SV40pro_F_pri ....... ..........

.......... .......... .......... .......... .......... ..........

2101 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg 2160

2101 gataaggtct tcatcactcc tccgaaaaaa cctccggatc cgaaaacgtt tttcgagggc 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 ggagcttgta tatccatttt cggatctgat cagcacgtgt tgacaattaa tcatcggcat 2220

2161 cctcgaacat ataggtaaaa gcctagacta gtcgtgcaca actgttaatt agtagccgta 2220

.......... .......... .......... .......... EM7_pro ..........

.......... .......... .......... .......... MscI(2268) ..........

2221 agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatggcc aagttgac ca 2280

2221 tcatatagcc gtatcatatt atgctgttcc actccttgat ttggtaccgg ttcaactggt 2280

.......... .......... EM7_pro .......... ......... bleo sh_ble

.......... .......... .......... .......... .......... ..........

2281 gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc 2340

2281 cacggcaagg ccacgagtgg cgcgcgctgc agcggcctcg ccagctcaag acctggctgg 2340

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg 2400

2341 ccgagcccaa gagggccctg aagcacctcc tgctgaagcg gccacaccag gccctgctgc 2400

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc ggacaacacc ctggcctggg 2460

2401 actgggacaa gtagtcgcgc caggtcctgg tccaccacgg cctgttgtgg gaccggaccc 2460

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact 2520

2461 acacccacgc gccggacctg ctcgacatgc ggctcaccag cctccagcac aggtgcttga 2520

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 tccgggacgc ctccgggccg gccatgaccg agatcggcga gcagccgtgg gggcgggagt 2580

2521 aggccctgcg gaggcccggc cggtactggc tctagccgct cgtcggcacc cccgccctca 2580

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag caggactgac 2640

2581 agcgggacgc gctgggccgg ccgttgacgc acgtgaagca ccggctcctc gtcctgactg 2640

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 acgtgctacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg 2700

2641 tgcacgatgc tctaaagcta aggtggcggc ggaagatact ttccaacccg aagccttagc 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg 2760

2701 aaaaggccct gcggccgacc tactaggagg tcgcgcccct agagtacgac ctcaagaagc 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa 2820

2761 gggtggggtt gaacaaataa cgtcgaatat taccaatgtt tatttcgtta tcgtagtgtt 2820

.......... .......... ......... SV40_PA_term ......... ..........

.......... .......... .......... .......... .......... ..........

2821 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc a aactcatca 2880

2821 taaagtgttt atttcgtaaa aaaagtgacg taagatcaac accaaacagg tttgagtagt 2880

.......... ......... SV40_PA_term ........ EBV_rev_pri .........

.......... .......... .......... .......... .......... ..........

2881 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt 2940

2881 tacatagaat agtacagaca tatggcagct ggagatcgat ctcgaaccgc attagtacca 2940

SV40_PA_term ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg 3000

2941 gtatcgacaa aggacacact ttaacaatag gcgagtgtta aggtgtgttg tatgctcggc 3000

M13_reverse_pri . M13_pUC_rev_pri ....... .......... lac_pro

.......... .......... .......... .......... .......... ..........

3001 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt 3060

3001 cttcgtattt cacatttcgg accccacgga ttactcactc gattgagtgt aattaacgca 3060

lac_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg 3120

3061 acgcgagtga cgggcgaaag gtcagccctt tggacagcac ggtcgacgta attacttagc 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg 3180

3121 cggttgcgcg cccctctccg ccaaacgcat aacccgcgag aaggcgaagg agcgagtgac 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa 3240

3181 tgagcgacgc gagccagcaa gccgacgccg ctcgccatag tcgagtgagt ttccgccatt 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc 3300

3241 atgccaatag gtgtcttagt cccctattgc gtcctttctt gtacactcgt tttccggtcg 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 3360

3301 ttttccggtc cttggcattt ttccggcgca acgaccgcaa aaaggtatcc gaggcggggg 3360

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

3361 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 3420

3361 gactgctcgt agtgttttta gctgcgagtt cagtctccac cgctttgggc tgtcctgata 3420

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3421 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 3480

3421 tttctatggt ccgcaaaggg ggaccttcga gggagcacgc gagaggacaa ggctgggacg 3480

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3481 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct 3540

3481 gcgaatggcc tatggacagg cggaaagagg gaagcccttc gcaccgcgaa agagtatcga 3540

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3541 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 3600

3541 gtgcgacatc catagagtca agccacatcc agcaagcgag gttcgacccg acacacgtgc 3600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3601 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 3660

3601 ttggggggca agtcgggctg gcgacgcgga ataggccatt gatagcagaa ctcaggttgg 3660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 3720

3661 gccattctgt gctgaatagc ggtgaccgtc gtcggtgacc attgtcctaa tcgtctcgct 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 3780

3721 ccatacatcc gccacgatgt ctcaagaact tcaccaccgg attgatgccg atgtgatctt 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 3840

3781 cttgtcataa accatagacg cgagacgact tcggtcaatg gaagcctttt tctcaaccat 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 3900

3841 cgagaactag gccgtttgtt tggtggcgac catcgccacc aaaaaaacaa acgttcgtcg 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 3960

3901 tctaatgcgc gtcttttttt cctagagttc ttctaggaaa ctagaaaaga tgccccagac 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 4020

3961 tgcgagtcac cttgcttttg agtgcaattc cctaaaacca gtactctaat agtttttcct 4020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 4080

4021 agaagtggat ctaggaaaat ttaattttta cttcaaaatt tagttagatt tcatatatac 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 4140

4081 tcatttgaac cagactgtca atggttacga attagtcact ccgtggatag agtcgctaga 4140

.......... .......... ....... ORF 2 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

4141 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 4200

4141 cagataaagc aagtaggtat caacggactg aggggcagca catctattga tgctatgccc 4200

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 4260

4201 tcccgaatgg tagaccgggg tcacgacgtt actatggcgc tctgggtgcg agtggccgag 4260

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 4320

4261 gtctaaatag tcgttatttg gtcggtcggc cttcccggct cgcgtcttca ccaggacgtt 4320

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 4380

4321 gaaataggcg gaggtaggtc agataattaa caacggccct tcgatctcat tcatcaagcg 4380

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... FspI(4395) .......... .......... .......... ..........

4381 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt 4440

4381 gtcaattatc aaacgcgttg caacaacggt aacgatgtcc gtagcaccac agtgcgagca 4440

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 4500

4441 gcaaaccata ccgaagtaag tcgaggccaa gggttgctag ttccgctcaa tgtactaggg 4500

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 4560

4501 ggtacaacac gttttttcgc caatcgagga agccaggagg ctagcaacag tcttcattca 4560

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 4620

4561 accggcgtca caatagtgag taccaatacc gtcgtgacgt attaagagaa tgacagtacg 4620

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 4680

4621 gtaggcattc tacgaaaaga cactgaccac tcatgagttg gttcagtaag actcttatca 4680

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata 4740

4681 catacgccgc tggctcaacg agaacgggcc gcagttatgc cctattatgg cgcggtgtat 4740

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 4800

4741 cgtcttgaaa ttttcacgag tagtaacctt ttgcaagaag ccccgctttt gagagttcct 4800

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 4860

4801 agaatggcga caactctagg tcaagctaca ttgggtgagc acgtgggttg actagaagtc 4860

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 4920

4861 gtagaaaatg aaagtggtcg caaagaccca ctcgtttttg tccttccgtt ttacggcgtt 4920

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 4980

4921 ttttccctta ttcccgctgt gcctttacaa cttatgagta tgagaaggaa aaagttataa 4980

....... ORF 2 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 5040

4981 taacttcgta aatagtccca ataacagagt actcgcctat gtataaactt acataaatct 5040

.......... .......... .......... AmpR_pro .......... ..........

.......... .......... .......... .......... .......... ..........

5041 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtc.... 5096

5041 ttttatttgt ttatccccaa ggcgcgtgta aaggggcttt tcacggtgga ctgcag.... 5096

.......... .......... .......... .......... .......... ..........