Sequence of pcDNA4_V5-His

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Sequence of pcDNA4_V5-His_C

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Sequence of pcDNA4/V5-His C : 5088 BP; 1184 A; 1326 C; 1286 G; 1292 T; 0 other;

.......... BglII(12) .......... .......... .......... ..........

1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 60

1 ctgcctagcc ctctagaggg ctaggggata ccacgtgaga gtcatgttag acgagactac 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120

61 ggcgtatcaa ttcggtcata gacgagggac gaacacacaa cctccagcga ctcatcacgc 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180

121 gctcgtttta aattcgatgt tgttccgttc cgaactggct gttaacgtac ttcttagacg 180

.......... .......... .......... .......... .......... ..........

.......... .......... NruI(208) .......... .......... ..........

181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240

181 aatcccaatc cgcaaaacgc gacgaagcgc tacatgcccg gtctatatgc gcaactgtaa 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 300

241 ctaataactg atcaataatt atcattagtt aatgccccag taatcaagta tcgggtatat 300

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 360

301 acctcaaggc gcaatgtatt gaatgccatt taccgggcgg accgactggc gggttgctgg 360

.......... ....... CMV_immearly_pro CAG_enh ....... ..........

.......... .......... .......... .......... .......... ..........

361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 420

361 gggcgggtaa ctgcagttat tactgcatac aagggtatca ttgcggttat ccctgaaagg 420

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 480

421 taactgcagt tacccacctc ataaatgcca tttgacgggt gaaccgtcat gtagttcaca 480

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

NdeI(484) .......... .......... .......... .......... ..........

481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 540

481 tagtatacgg ttcatgcggg ggataactgc agttactgcc atttaccggg cggaccgtaa 540

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 600

541 tacgggtcat gtactggaat accctgaaag gatgaaccgt catgtagatg cataatcagt 600

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 660

601 agcgataatg gtaccactac gccaaaaccg tcatgtagtt acccgcacct atcgccaaac 660

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 720

661 tgagtgcccc taaaggttca gaggtggggt aactgcagtt accctcaaac aaaaccgtgg 720

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 780

721 ttttagttgc cctgaaaggt tttacagcat tgttgaggcg gggtaactgc gtttacccgc 780

.......... ....... CMV_immearly_pro ....... ......... CMV_fwd_pri

.......... .......... .......... .......... .......... ..........

781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 840

781 catccgcaca tgccaccctc cagatatatt cgtctcgaga gaccgattga tctcttgggt 840

CMV_fwd_pri ..... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 900

841 gacgaatgac cgaatagctt taattatgct gagtgatatc cctctgggtt cgaccgatcg 900

CMV_immearly_pro .... .......... T7_pro .......... ..........

AflII(908),HindIII(911) KpnI(921) BamHI(929) ...... ..........

901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattctgc 960

901 caaatttgaa ttcgaaccat ggctcgagcc taggtgatca ggtcacacca ccttaagacg 960

.......... .......... .......... .......... .......... ..........

PstI1),EcoRV(964) NotI(979) XhoI(985) BstBI(1000) ........

961 agatatccag cacagtggcg gccgctcgag gtcacccatt cgaaggtaag cctatcccta 1020

961 tctataggtc gtgtcaccgc cggcgagctc cagtgggtaa gcttccattc ggatagggat 1020

.......... .......... .......... .......... .......... V5

.......... .......... AgeI(1050) .......... .......... ..........

1021 accctctcct cggtctcgat tctacgcgta ccggtcatca tcaccatcac cattgagttt 1080

1021 tgggagagga gccagagcta agatgcgcat ggccagtagt agtggtagtg gtaactcaaa 1080

.......... V5 .......... .......... 6xHis ..........

.......... .......... .......... .......... .......... ..........

1081 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct 1140

1081 tttgggcgac tagtcggagc tgacacggaa gatcaacggt cggtagacaa caaacgggga 1140

.......... ........ BGH_rev_pri bGH_PA_term ........ ..........

.......... .......... .......... .......... .......... ..........

1141 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg 1200

1141 gggggcacgg aaggaactgg gaccttccac ggtgagggtg acaggaaagg attattttac 1200

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc 1260

1201 tcctttaacg tagcgtaaca gactcatcca cagtaagata agacccccca ccccaccccg 1260

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct 1320

1261 tcctgtcgtt ccccctccta acccttctgt tatcgtccgt acgaccccta cgccacccga 1320

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct 1380

1321 gataccgaag actccgcctt tcttggtcga ccccgagatc ccccataggg gtgcgcggga 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg 1440

1381 catcgccgcg taattcgcgc cgcccacacc accaatgcgc gtcgcactgg cgatgtgaac 1440

.......... .......... .......... f1_origin .......... ..........

.......... .......... .......... .......... .......... ..........

1441 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg 1500

1441 ggtcgcggga tcgcgggcga ggaaagcgaa agaagggaag gaaagagcgg tgcaagcggc 1500

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac 1560

1501 cgaaaggggc agttcgagat ttagcccccg agggaaatcc caaggctaaa tcacgaaatg 1560

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct 1620

1561 ccgtggagct ggggtttttt gaactaatcc cactaccaag tgcatcaccc ggtagcggga 1620

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt 1680

1621 ctatctgcca aaaagcggga aactgcaacc tcaggtgcaa gaaattatca cctgagaaca 1680

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt 1740

1681 aggtttgacc ttgttgtgag ttgggataga gccagataag aaaactaaat attccctaaa 1740

f1_origin .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt 1800

1741 acggctaaag ccggataacc aattttttac tcgactaaat tgtttttaaa ttgcgcttaa 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag 1860

1801 ttaagacacc ttacacacag tcaatcccac acctttcagg ggtccgaggg gtcgtccgtc 1860

......... pBABE_3_pri ......... SV40_enh SV40_pro ..........

.......... .......... .......... .......... .......... ..........

1861 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc 1920

1861 ttcatacgtt tcgtacgtag agttaatcag tcgttggtcc acacctttca ggggtccgag 1920

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc 1980

1921 gggtcgtccg tcttcatacg tttcgtacgt agagttaatc agtcgttggt atcagggcgg 1980

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg 2040

1981 ggattgaggc gggtagggcg gggattgagg cgggtcaagg cgggtaagag gcggggtacc 2040

.......... ..... SV40_enh SV40_pro SV40_origin ..... ..........

.......... .......... .......... .......... .......... ..........

2041 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca 2100

2041 gactgattaa aaaaaataaa tacgtctccg gctccggcgg agacggagac tcgataaggt 2100

... SV40_origin SV40_pro SV40pro_F_pri ... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg 2160

2101 cttcatcact cctccgaaaa aacctccgga tccgaaaacg tttttcgagg gccctcgaac 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc atagtatatc 2220

2161 atataggtaa aagcctagac tagtcgtgca caactgttaa ttagtagccg tatcatatag 2220

.......... .......... .......... .......... EM7_pro ..........

.......... .......... .......... MscI(2260) .......... ..........

2221 ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagttgac < font style = "background-color:#C94300">cagtgccgtt 2280

2221 ccgtatcata ttatgctgtt ccactccttg atttggtacc ggttcaactg gtcacggcaa 2280

.......... EM7_pro .......... ......... bleo sh_ble .........

.......... .......... .......... .......... .......... ..........

2281 ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga ccggctcggg 2340

2281 ggccacgagt ggcgcgcgct gcagcggcct cgccagctca agacctggct ggccgagccc 2340

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 ttctcccggg acttcgtgga ggacgacttc gccggtgtgg tccgggacga cgtgaccctg 2400

2341 aagagggccc tgaagcacct cctgctgaag cggccacacc aggccctgct gcactgggac 2400

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 ttcatcagcg cggtccagga ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg 2460

2401 aagtagtcgc gccaggtcct ggtccaccac ggcctgttgt gggaccggac ccacacccac 2460

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac 2520

2461 gcgccggacc tgctcgacat gcggctcacc agcctccagc acaggtgctt gaaggccctg 2520

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 gcctccgggc cggccatgac cgagatcggc gagcagccgt gggggcggga gttcgccctg 2580

2521 cggaggcccg gccggtactg gctctagccg ctcgtcggca cccccgccct caagcgggac 2580

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg agcaggactg acacgtgcta 2640

2581 gcgctgggcc ggccgttgac gcacgtgaag caccggctcc tcgtcctgac tgtgcacgat 2640

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg 2700

2641 gctctaaagc taaggtggcg gcggaagata ctttccaacc cgaagcctta gcaaaaggcc 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc 2760

2701 ctgcggccga cctactagga ggtcgcgccc ctagagtacg acctcaagaa gcgggtgggg 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca 2820

2761 ttgaacaaat aacgtcgaat attaccaatg tttatttcgt tatcgtagtg tttaaagtgt 2820

.......... .......... ......... SV40_PA_term ......... ..........

.......... .......... .......... .......... .......... ..........

2821 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat c aatgtatct 2880

2821 ttatttcgta aaaaaagtga cgtaagatca acaccaaaca ggtttgagta gttacataga 2880

.......... ......... SV40_PA_term ........ EBV_rev_pri .........

.......... .......... .......... .......... .......... ..........

2881 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg 2940

2881 atagtacaga catatggcag ctggagatcg atctcgaacc gcattagtac cagtatcgac 2940

.......... .......... .......... .......... ....... M13_reverse_pri

.......... .......... .......... .......... .......... ..........

2941 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata 3000

2941 aaaggacaca ctttaacaat aggcgagtgt taaggtgtgt tgtatgctcg gccttcgtat 3000

....... M13_pUC_rev_pri ....... .......... lac_pro ..........

.......... .......... .......... .......... .......... ..........

3001 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca 3060

3001 ttcacatttc ggaccccacg gattactcac tcgattgagt gtaattaacg caacgcgagt 3060

lac_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc 3120

3061 gacgggcgaa aggtcagccc tttggacagc acggtcgacg taattactta gccggttgcg 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg 3180

3121 cgcccctctc cgccaaacgc ataacccgcg agaaggcgaa ggagcgagtg actgagcgac 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta 3240

3181 gcgagccagc aagccgacgc cgctcgccat agtcgagtga gtttccgcca ttatgccaat 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc 3300

3241 aggtgtctta gtcccctatt gcgtcctttc ttgtacactc gttttccggt cgttttccgg 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag 3360

3301 tccttggcat ttttccggcg caacgaccgc aaaaaggtat ccgaggcggg gggactgctc 3360

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

3361 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac 3420

3361 gtagtgtttt tagctgcgag ttcagtctcc accgctttgg gctgtcctga tatttctatg 3420

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3421 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 3480

3421 gtccgcaaag ggggaccttc gagggagcac gcgagaggac aaggctggga cggcgaatgg 3480

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3481 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt 3540

3481 cctatggaca ggcggaaaga gggaagccct tcgcaccgcg aaagagtatc gagtgcgaca 3540

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3541 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc 3600

3541 tccatagagt caagccacat ccagcaagcg aggttcgacc cgacacacgt gcttgggggg 3600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3601 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga 3660

3601 caagtcgggc tggcgacgcg gaataggcca ttgatagcag aactcaggtt gggccattct 3660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta 3720

3661 gtgctgaata gcggtgaccg tcgtcggtga ccattgtcct aatcgtctcg ctccatacat 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta 3780

3721 ccgccacgat gtctcaagaa cttcaccacc ggattgatgc cgatgtgatc ttcttgtcat 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga 3840

3781 aaaccataga cgcgagacga cttcggtcaa tggaagcctt tttctcaacc atcgagaact 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg 3900

3841 aggccgtttg tttggtggcg accatcgcca ccaaaaaaac aaacgttcgt cgtctaatgc 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag 3960

3901 gcgtcttttt ttcctagagt tcttctagga aactagaaaa gatgccccag actgcgagtc 3960

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc 4020

3961 accttgcttt tgagtgcaat tccctaaaac cagtactcta atagtttttc ctagaagtgg 4020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact 4080

4021 atctaggaaa atttaatttt tacttcaaaa tttagttaga tttcatatat actcatttga 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt 4140

4081 accagactgt caatggttac gaattagtca ctccgtggat agagtcgcta gacagataaa 4140

.......... .......... ....... ORF 3 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

4141 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta 4200

4141 gcaagtaggt atcaacggac tgaggggcag cacatctatt gatgctatgc cctcccgaat 4200

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta 4260

4201 ggtagaccgg ggtcacgacg ttactatggc gctctgggtg cgagtggccg aggtctaaat 4260

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc 4320

4261 agtcgttatt tggtcggtcg gccttcccgg ctcgcgtctt caccaggacg ttgaaatagg 4320

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat 4380

4321 cggaggtagg tcagataatt aacaacggcc cttcgatctc attcatcaag cggtcaatta 4380

.......... ....... ORF 3 Ampicillin ....... .......... ..........

FspI(4387) .......... .......... .......... .......... ..........

4381 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt 4440

4381 tcaaacgcgt tgcaacaacg gtaacgatgt ccgtagcacc acagtgcgag cagcaaacca 4440

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg 4500

4441 taccgaagta agtcgaggcc aagggttgct agttccgctc aatgtactag ggggtacaac 4500

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca 4560

4501 acgttttttc gccaatcgag gaagccagga ggctagcaac agtcttcatt caaccggcgt 4560

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta 4620

4561 cacaatagtg agtaccaata ccgtcgtgac gtattaagag aatgacagta cggtaggcat 4620

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg 4680

4621 tctacgaaaa gacactgacc actcatgagt tggttcagta agactcttat cacatacgcc 4680

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact 4740

4681 gctggctcaa cgagaacggg ccgcagttat gccctattat ggcgcggtgt atcgtcttga 4740

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg 4800

4741 aattttcacg agtagtaacc ttttgcaaga agccccgctt ttgagagttc ctagaatggc 4800

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt 4860

4801 gacaactcta ggtcaagcta cattgggtga gcacgtgggt tgactagaag tcgtagaaaa 4860

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga 4920

4861 tgaaagtggt cgcaaagacc cactcgtttt tgtccttccg ttttacggcg ttttttccct 4920

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc 4980

4921 tattcccgct gtgcctttac aacttatgag tatgagaagg aaaaagttat aataacttcg 4980

....... ORF 3 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa 5040

4981 taaatagtcc caataacaga gtactcgcct atgtataaac ttacataaat ctttttattt 5040

.......... .......... AmpR_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtc.. .......... 5088

5041 gtttatcccc aaggcgcgtg taaaggggct tttcacggtg gactgcag.. .......... 5088

.......... .......... .......... .......... .......... ..........