Sequence of pcDNA4_myc-His

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Sequence of pcDNA4_myc-His_A

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Sequence of pcDNA4/myc-His A : 5075 BP; 1189 A; 1313 C; 1288 G; 1285 T; 0 other;

.......... BglII(12) .......... .......... .......... ..........

1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 60

1 ctgcctagcc ctctagaggg ctaggggata ccagctgaga gtcatgttag acgagactac 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120

61 ggcgtatcaa ttcggtcata gacgagggac gaacacacaa cctccagcga ctcatcacgc 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180

121 gctcgtttta aattcgatgt tgttccgttc cgaactggct gttaacgtac ttcttagacg 180

.......... .......... .......... .......... .......... ..........

.......... .......... NruI(208) .......... .......... ..........

181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240

181 aatcccaatc cgcaaaacgc gacgaagcgc tacatgcccg gtctatatgc gcaactgtaa 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 300

241 ctaataactg atcaataatt atcattagtt aatgccccag taatcaagta tcgggtatat 300

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 360

301 acctcaaggc gcaatgtatt gaatgccatt taccgggcgg accgactggc gggttgctgg 360

.......... ....... CMV_immearly_pro CAG_enh ....... ..........

.......... .......... .......... .......... .......... ..........

361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 420

361 gggcgggtaa ctgcagttat tactgcatac aagggtatca ttgcggttat ccctgaaagg 420

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 480

421 taactgcagt tacccacctg ataaatgcca tttgacgggt gaaccgtcat gtagttcaca 480

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

NdeI(484) .......... .......... .......... .......... ..........

481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 540

481 tagtatacgg ttcatgcggg ggataactgc agttactgcc atttaccggg cggaccgtaa 540

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 600

541 tacgggtcat gtactggaat accctgaaag gatgaaccgt catgtagatg cataatcagt 600

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 660

601 agcgataatg gtaccactac gccaaaaccg tcatgtagtt acccgcacct atcgccaaac 660

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 720

661 tgagtgcccc taaaggttca gaggtggggt aactgcagtt accctcaaac aaaaccgtgg 720

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 780

721 ttttagttgc cctgaaaggt tttacagcat tgttgaggcg gggtaactgc gtttacccgc 780

.......... ....... CMV_immearly_pro ....... ......... CMV_fwd_pri

.......... .......... .......... .......... .......... ..........

781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 840

781 catccgcaca tgccaccctc cagatatatt cgtctcgaga gaccgattga tctcttgggt 840

CMV_fwd_pri ..... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt 900

841 gacgaatgac cgaatagctt taattatgct gagtgatatc cctctgggtt cgaccgatca 900

CMV_immearly_pro .... .......... T7_pro .......... ..........

HindIII(902) KpnI(912) BamHI(920) ........ EcoRI(943) ..........

901 taagcttggt accgagctcg gatccactag tccagtgtgg tggaattctg cagatatcca 960

901 attcgaacca tggctcgagc ctaggtgatc aggtcacacc accttaagac gtctataggt 960

.......... .......... .......... .......... .......... ..........

NotI(970) XhoI(976) XbaI(982) .......... .......... ..........

961 gcacagtggc ggccgctcga gtctagaggg cccttcgaac aaaaactcat ctcagaagag 1020

961 cgtgtcaccg ccggcgagct cagatctccc gggaagcttg tttttgagta gagtcttctc 1020

.......... .......... .......... .......... c_myc ..........

.......... AgeI(1036) .......... .......... .......... ..........

1021 gatctgaata tgcataccgg tcatcatcac catcaccatt gagtttaaac ccgctgatca 1080

1021 ctagacttat acgtatggcc agtagtagtg gtagtggtaa ctcaaatttg ggcgactagt 1080

.......... .......... 6xHis .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc 1140

1081 cggagctgac acggaagatc aacggtcggt agacaacaaa cggggagggg gcacggaagg 1140

BGH_rev_pri ........ ......... bGH_PA_term ......... ..........

.......... .......... .......... .......... .......... ..........

1141 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg 1200

1141 aactgggacc ttccacggtg agggtgacag gaaaggatta ttttactcct ttaacgtagc 1200

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg 1260

1201 gtaacagact catccacagt aagataagac cccccacccc accccgtcct gtcgttcccc 1260

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag 1320

1261 ctcctaaccc ttctgttatc gtccgtacga cccctacgcc acccgagata ccgaagactc 1320

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta 1380

1321 cgcctttctt ggtcgacccc gagatccccc ataggggtgc gcgggacatc gccgcgtaat 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg 1440

1381 tcgcgccgcc cacaccacca atgcgcgtcg cactggcgat gtgaacggtc gcgggatcgc 1440

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa 1500

1441 gggcgaggaa agcgaaagaa gggaaggaaa gagcggtgca agcggccgaa aggggcagtt 1500

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 gctctaaatc ggggcatccc tttagggttc cgatttagtg ctttacggca cctcgacccc 1560

1501 cgagatttag ccccgtaggg aaatcccaag gctaaatcac gaaatgccgt ggagctgggg 1560

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt 1620

1561 ttttttgaac taatcccact accaagtgca tcacccggta gcgggactat ctgccaaaaa 1620

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca 1680

1621 gcgggaaact gcaacctcag gtgcaagaaa ttatcacctg agaacaaggt ttgaccttgt 1680

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 acactcaacc ctatctcggt ctattctttt gatttataag ggattttggg gatttcggcc 1740

1681 tgtgagttgg gatagagcca gataagaaaa ctaaatattc cctaaaaccc ctaaagccgg 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg 1800

1741 ataaccaatt ttttactcga ctaaattgtt tttaaattgc gcttaattaa gacaccttac 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 tgtgtcagtt agggtgtgga aagtccccag gctccccagg caggcagaag tatgcaaagc 1860

1801 acacagtcaa tcccacacct ttcaggggtc cgaggggtcc gtccgtcttc atacgtttcg 1860

pBABE_3_pri ........ SV40_enh SV40_pro .......... ..........

.......... .......... .......... .......... .......... ..........

1861 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga 1920

1861 tacgtagagt taatcagtcg ttggtccaca cctttcaggg gtccgagggg tcgtccgtct 1920

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc 1980

1921 tcatacgttt cgtacgtaga gttaatcagt cgttggtatc agggcgggga ttgaggcggg 1980

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt 2040

1981 tagggcgggg attgaggcgg gtcaaggcgg gtaagaggcg gggtaccgac tgattaaaaa 2040

..... SV40_enh SV40_pro SV40_origin ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga 2100

2041 aaataaatac gtctccggct ccggcggaga cggagactcg ataaggtctt catcactcct 2100

SV40_origin SV40pro_F_pri SV40_pro .... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc 2160

2101 ccgaaaaaac ctccggatcc gaaaacgttt ttcgagggcc ctcgaacata taggtaaaag 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat 2220

2161 cctagactag tcgtgcacaa ctgttaatta gtagccgtat catatagccg tatcatatta 2220

.......... .......... .......... EM7_pro .......... ..........

.......... .......... MscI(2247) .......... .......... ..........

2221 acgacaaggt gaggaactaa accatggcca agttgaccag tgccgttccg gtgctcaccg 2280

2221 tgctgttcca ctccttgatt tggtaccggt tcaactggtc acggcaaggc cacgagtggc 2280

.......... EM7_pro .......... ......... bleo sh_ble .........

.......... .......... .......... .......... .......... ..........

2281 cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact 2340

2281 gcgcgctgca gcggcctcgc cagctcaaga cctggctggc cgagcccaag agggccctga 2340

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg 2400

2341 agcacctcct gctgaagcgg ccacaccagg ccctgctgca ctgggacaag tagtcgcgcc 2400

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 tccaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg 2460

2401 aggtcctggt ccaccacggc ctgttgtggg accggaccca cacccacgcg ccggacctgc 2460

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg 2520

2461 tcgacatgcg gctcaccagc ctccagcaca ggtgcttgaa ggccctgcgg aggcccggcc 2520

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg 2580

2521 ggtactggct ctagccgctc gtcggcaccc ccgccctcaa gcgggacgcg ctgggccggc 2580

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 gcaactgcgt gcacttcgtg gccgaggagc aggactgaca cgtgctacga gatttcgatt 2640

2581 cgttgacgca cgtgaagcac cggctcctcg tcctgactgt gcacgatgct ctaaagctaa 2640

......... bleo sh_ble ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga 2700

2641 ggtggcggcg gaagatactt tccaacccga agccttagca aaaggccctg cggccgacct 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg 2760

2701 actaggaggt cgcgccccta gagtacgacc tcaagaagcg ggtggggttg aacaaataac 2760

.......... .......... .......... .......... ......... SV40_PA_term

.......... .......... .......... .......... .......... ..........

2761 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt 2820

2761 gtcgaatatt accaatgttt atttcgttat cgtagtgttt aaagtgttta tttcgtaaaa 2820

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta 2880

2821 aaagtgacgt aagatcaaca ccaaacaggt ttgagtagtt acatagaata gtacagacat 2880

.......... ... SV40_PA_term EBV_rev_pri ... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa 2940

2881 atggcagctg gagatcgatc tcgaaccgca ttagtaccag tatcgacaaa ggacacactt 2940

.......... .......... .......... .... M13_reverse_pri M13_pUC_rev_pri

.......... .......... .......... .......... .......... ..........

2941 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct 3000

2941 taacaatagg cgagtgttaa ggtgtgttgt atgctcggcc ttcgtatttc acatttcgga 3000

M13_pUC_rev_pri .... .......... .......... lac_pro ..........

.......... .......... .......... .......... .......... ..........

3001 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc 3060

3001 ccccacggat tactcactcg attgagtgta attaacgcaa cgcgagtgac gggcgaaagg 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg 3120

3061 tcagcccttt ggacagcacg gtcgacgtaa ttacttagcc ggttgcgcgc ccctctccgc 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 3180

3121 caaacgcata acccgcgaga aggcgaagga gcgagtgact gagcgacgcg agccagcaag 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 3240

3181 ccgacgccgc tcgccatagt cgagtgagtt tccgccatta tgccaatagg tgtcttagtc 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 3300

3241 ccctattgcg tcctttcttg tacactcgtt ttccggtcgt tttccggtcc ttggcatttt 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 3360

3301 tccggcgcaa cgaccgcaaa aaggtatccg aggcgggggg actgctcgta gtgtttttag 3360

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

3361 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 3420

3361 ctgcgagttc agtctccacc gctttgggct gtcctgatat ttctatggtc cgcaaagggg 3420

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3421 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 3480

3421 gaccttcgag ggagcacgcg agaggacaag gctgggacgg cgaatggcct atggacaggc 3480

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3481 cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt 3540

3481 ggaaagaggg aagcccttcg caccgcgaaa gagttacgag tgcgacatcc atagagtcaa 3540

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3541 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 3600

3541 gccacatcca gcaagcgagg ttcgacccga cacacgtgct tggggggcaa gtcgggctgg 3600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3601 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 3660

3601 cgacgcggaa taggccattg atagcagaac tcaggttggg ccattctgtg ctgaatagcg 3660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 3720

3661 gtgaccgtcg tcggtgacca ttgtcctaat cgtctcgctc catacatccg ccacgatgtc 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg 3780

3721 tcaagaactt caccaccgga ttgatgccga tgtgatcttc ctgtcataaa ccatagacgc 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 3840

3781 gagacgactt cggtcaatgg aagccttttt ctcaaccatc gagaactagg ccgtttgttt 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 3900

3841 ggtggcgacc atcgccacca aaaaaacaaa cgttcgtcgt ctaatgcgcg tctttttttc 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 3960

3901 ctagagttct tctaggaaac tagaaaagat gccccagact gcgagtcacc ttgcttttga 3960

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 4020

3961 gtgcaattcc ctaaaaccag tactctaata gtttttccta gaagtggatc taggaaaatt 4020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 4080

4021 taatttttac ttcaaaattt agttagattt catatatact catttgaacc agactgtcaa 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 4140

4081 tggttacgaa ttagtcactc cgtggataga gtcgctagac agataaagca agtaggtatc 4140

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 4200

4141 aacggactga ggggcagcac atctattgat gctatgccct cccgaatggt agaccggggt 4200

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 4260

4201 cacgacgtta ctatggcgct ctgggtgcga gtggccgagg tctaaatagt cgttatttgg 4260

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 4320

4261 tcggtcggcc ttcccggctc gcgtcttcac caggacgttg aaataggcgg aggtaggtca 4320

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... FspI(4374)

4321 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 4380

4321 gataattaac aacggccctt cgatctcatt catcaagcgg tcaattatca aacgcgttgc 4380

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 4440

4381 aacaacggta acgatgtccg tagcaccaca gtgcgagcag caaaccatac cgaagtaagt 4440

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 4500

4441 cgaggccaag ggttgctagt tccgctcaat gtactagggg gtacaacacg ttttttcgcc 4500

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 4560

4501 aatcgaggaa gccaggaggc tagcaacagt cttcattcaa ccggcgtcac aatagtgagt 4560

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 4620

4561 accaataccg tcgtgacgta ttaagagaat gacagtacgg taggcattct acgaaaagac 4620

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 4680

4621 actgaccact catgagttgg ttcagtaaga ctcttatcac atacgccgct ggctcaacga 4680

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 4740

4681 gaacgggccg cagttatgcc ctattatggc gcggtgtatc gtcttgaaat tttcacgagt 4740

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 4800

4741 agtaaccttt tgcaagaagc cccgcttttg agagttccta gaatggcgac aactctaggt 4800

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 4860

4801 caagctacat tgggtgagca cgtgggttga ctagaagtcg tagaaaatga aagtggtcgc 4860

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 4920

4861 aaagacccac tcgtttttgt ccttccgttt tacggcgttt tttcccttat tcccgctgtg 4920

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 4980

4921 cctttacaac ttatgagtat gagaaggaaa aagttataat aacttcgtaa atagtcccaa 4980

ORF 2 Ampicillin .... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 5040

4981 taacagagta ctcgcctatg tataaactta cataaatctt tttatttgtt tatccccaag 5040

.......... AmpR_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 cgcgcacatt tccccgaaaa gtgccacctg acgtc..... .......... .......... 5075

5041 gcgcgtgtaa aggggctttt cacggtggac tgcag..... .......... .......... 5075

.......... .......... .......... .......... .......... ..........