Sequence of pcDNA4_myc-His

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Sequence of pcDNA4_myc-His_C

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Sequence of pcDNA4/myc-His C : 5071 BP; 1189 A; 1313 C; 1285 G; 1284 T; 0 other;

.......... BglII(12) .......... .......... .......... ..........

1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 60

1 ctgcctagcc ctctagaggg ctaggggata ccagctgaga gtcatgttag acgagactac 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120

61 ggcgtatcaa ttcggtcata gacgagggac gaacacacaa cctccagcga ctcatcacgc 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180

121 gctcgtttta aattcgatgt tgttccgttc cgaactggct gttaacgtac ttcttagacg 180

.......... .......... .......... .......... .......... ..........

.......... .......... NruI(208) .......... .......... ..........

181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240

181 aatcccaatc cgcaaaacgc gacgaagcgc tacatgcccg gtctatatgc gcaactgtaa 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 300

241 ctaataactg atcaataatt atcattagtt aatgccccag taatcaagta tcgggtatat 300

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 360

301 acctcaaggc gcaatgtatt gaatgccatt taccgggcgg accgactggc gggttgctgg 360

.......... ....... CMV_immearly_pro CAG_enh ....... ..........

.......... .......... .......... .......... .......... ..........

361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 420

361 gggcgggtaa ctgcagttat tactgcatac aagggtatca ttgcggttat ccctgaaagg 420

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 480

421 taactgcagt tacccacctg ataaatgcca tttgacgggt gaaccgtcat gtagttcaca 480

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

NdeI(484) .......... .......... .......... .......... ..........

481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 540

481 tagtatacgg ttcatgcggg ggataactgc agttactgcc atttaccggg cggaccgtaa 540

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 600

541 tacgggtcat gtactggaat accctgaaag gatgaaccgt catgtagatg cataatcagt 600

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 660

601 agcgataatg gtaccactac gccaaaaccg tcatgtagtt acccgcacct atcgccaaac 660

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 720

661 tgagtgcccc taaaggttca gaggtggggt aactgcagtt accctcaaac aaaaccgtgg 720

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 780

721 ttttagttgc cctgaaaggt tttacagcat tgttgaggcg gggtaactgc gtttacccgc 780

.......... ....... CMV_immearly_pro ....... ......... CMV_fwd_pri

.......... .......... .......... .......... .......... ..........

781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 840

781 catccgcaca tgccaccctc cagatatatt cgtctcgaga gaccgattga tctcttgggt 840

CMV_fwd_pri ..... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt 900

841 gacgaatgac cgaatagctt taattatgct gagtgatatc cctctgggtt cgaccgatca 900

CMV_immearly_pro .... .......... T7_pro .......... ..........

HindIII(902) KpnI(912) BamHI(920) ........ EcoRI(943) ..........

901 taagcttggt accgagctcg gatccactag tccagtgtgg tggaattctg cagatatcca 960

901 attcgaacca tggctcgagc ctaggtgatc aggtcacacc accttaagac gtctataggt 960

.......... .......... .......... .......... .......... ..........

NotI(970) XhoI(976) .......... .......... .......... ..........

961 gcacagtggc ggccgctcga ggtcacccat tcgaacaaaa actcatctca gaagaggatc 1020

961 cgtgtcaccg ccggcgagct ccagtgggta agcttgtttt tgagtagagt cttctcctag 1020

.......... .......... .......... .......... c_myc ..........

.......... AgeI(1032) .......... .......... .......... ..........

1021 tgaatatgca taccggtcat catcaccatc accattgagt ttaaacccgc tgatcagcct 1080

1021 acttatacgt atggccagta gtagtggtag tggtaactca aatttgggcg actagtcgga 1080

.......... .......... 6xHis .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga 1140

1081 gctgacacgg aagatcaacg gtcggtagac aacaaacggg gagggggcac ggaaggaact 1140

BGH_rev_pri ....... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt 1200

1141 gggaccttcc acggtgaggg tgacaggaaa ggattatttt actcctttaa cgtagcgtaa 1200

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg 1260

1201 cagactcatc cacagtaaga taagaccccc caccccaccc cgtcctgtcg ttccccctcc 1260

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg 1320

1261 taacccttct gttatcgtcc gtacgacccc tacgccaccc gagataccga agactccgcc 1320

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg 1380

1321 tttcttggtc gaccccgaga tcccccatag gggtgcgcgg gacatcgccg cgtaattcgc 1380

.......... .......... .......... .......... .......... f1_origin

.......... .......... .......... .......... .......... ..........

1381 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg 1440

1381 gccgcccaca ccaccaatgc gcgtcgcact ggcgatgtga acggtcgcgg gatcgcgggc 1440

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc 1500

1441 gaggaaagcg aaagaaggga aggaaagagc ggtgcaagcg gccgaaaggg gcagttcgag 1500

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 taaatcgggg catcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa 1560

1501 atttagcccc gtagggaaat cccaaggcta aatcacgaaa tgccgtggag ctggggtttt 1560

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc 1620

1561 ttgaactaat cccactacca agtgcatcac ccggtagcgg gactatctgc caaaaagcgg 1620

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac 1680

1621 gaaactgcaa cctcaggtgc aagaaattat cacctgagaa caaggtttga ccttgttgtg 1680

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 tcaaccctat ctcggtctat tcttttgatt tataagggat tttggggatt tcggcctatt 1740

1681 agttgggata gagccagata agaaaactaa atattcccta aaacccctaa agccggataa 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg 1800

1741 ccaatttttt actcgactaa attgttttta aattgcgctt aattaagaca ccttacacac 1800

.......... .......... .......... .......... ......... pBABE_3_pri

.......... .......... .......... .......... .......... ..........

1801 tcagttaggg tgtggaaagt ccccaggctc cccaggcagg cagaagtatg caaagcatgc 1860

1801 agtcaatccc acacctttca ggggtccgag gggtccgtcc gtcttcatac gtttcgtacg 1860

pBABE_3_pri ........ SV40_enh SV40_pro .......... ..........

.......... .......... .......... .......... .......... ..........

1861 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta 1920

1861 tagagttaat cagtcgttgg tccacacctt tcaggggtcc gaggggtcgt ccgtcttcat 1920

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc 1980

1921 acgtttcgta cgtagagtta atcagtcgtt ggtatcaggg cggggattga ggcgggtagg 1980

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta 2040

1981 gcggggattg aggcgggtca aggcgggtaa gaggcggggt accgactgat taaaaaaaat 2040

..... SV40_enh SV40_pro SV40_origin ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct 2100

2041 aaatacgtct ccggctccgg cggagacgga gactcgataa ggtcttcatc actcctccga 2100

SV40_origin SV40pro_F_pri SV40_pro .... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat 2160

2101 aaaaacctcc ggatccgaaa acgtttttcg agggccctcg aacatatagg taaaagccta 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 ctgatcagca cgtgttgaca attaatcatc ggcatagtat atcggcatag tataatacga 2220

2161 gactagtcgt gcacaactgt taattagtag ccgtatcata tagccgtatc atattatgct 2220

.......... .......... .......... EM7_pro .......... ..........

.......... .......... MscI(2243) .......... .......... ..........

2221 caaggtgagg aactaaacca tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg 2280

2221 gttccactcc ttgatttggt accggttcaa ctggtcacgg caaggccacg agtggcgcgc 2280

EM7_pro .......... ......... bleo sh_ble ......... ..........

.......... .......... .......... .......... .......... ..........

2281 cgacgtcgcc ggagcggtcg agttctggac cgaccggctc gggttctccc gggacttcgt 2340

2281 gctgcagcgg cctcgccagc tcaagacctg gctggccgag cccaagaggg ccctgaagca 2340

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 ggaggacgac ttcgccggtg tggtccggga cgacgtgacc ctgttcatca gcgcggtcca 2400

2341 cctcctgctg aagcggccac accaggccct gctgcactgg gacaagtagt cgcgccaggt 2400

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct 2460

2401 cctggtccac cacggcctgt tgtgggaccg gacccacacc cacgcgccgg acctgctcga 2460

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 gtacgccgag tggtcggagg tcgtgtccac gaacttccgg gacgcctccg ggccggccat 2520

2461 catgcggctc accagcctcc agcacaggtg cttgaaggcc ctgcggaggc ccggccggta 2520

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa 2580

2521 ctggctctag ccgctcgtcg gcacccccgc cctcaagcgg gacgcgctgg gccggccgtt 2580

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ctgcgtgcac ttcgtggccg aggagcagga ctgacacgtg ctacgagatt tcgattccac 2640

2581 gacgcacgtg aagcaccggc tcctcgtcct gactgtgcac gatgctctaa agctaaggtg 2640

......... bleo sh_ble ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat 2700

2641 gcggcggaag atactttcca acccgaagcc ttagcaaaag gccctgcggc cgacctacta 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc 2760

2701 ggaggtcgcg cccctagagt acgacctcaa gaagcgggtg gggttgaaca aataacgtcg 2760

.......... .......... .......... .......... ......... SV40_PA_term

.......... .......... .......... .......... .......... ..........

2761 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc 2820

2761 aatattacca atgtttattt cgttatcgta gtgtttaaag tgtttatttc gtaaaaaaag 2820

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc 2880

2821 tgacgtaaga tcaacaccaa acaggtttga gtagttacat agaatagtac agacatatgg 2880

.......... ... SV40_PA_term EBV_rev_pri ... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg 2940

2881 cagctggaga tcgatctcga accgcattag taccagtatc gacaaaggac acactttaac 2940

.......... .......... ....... M13_reverse_pri .... M13_pUC_rev_pri

.......... .......... .......... .......... .......... ..........

2941 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg 3000

2941 aataggcgag tgttaaggtg tgttgtatgc tcggccttcg tatttcacat ttcggacccc 3000

M13_pUC_rev_pri .... .......... lac_pro .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc 3060

3001 acggattact cactcgattg agtgtaatta acgcaacgcg agtgacgggc gaaaggtcag 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt 3120

3061 ccctttggac agcacggtcg acgtaattac ttagccggtt gcgcgcccct ctccgccaaa 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 3180

3121 cgcataaccc gcgagaaggc gaaggagcga gtgactgagc gacgcgagcc agcaagccga 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 3240

3181 cgccgctcgc catagtcgag tgagtttccg ccattatgcc aataggtgtc ttagtcccct 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 3300

3241 attgcgtcct ttcttgtaca ctcgttttcc ggtcgttttc cggtccttgg catttttccg 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 3360

3301 gcgcaacgac cgcaaaaagg tatccgaggc ggggggactg ctcgtagtgt ttttagctgc 3360

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3361 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 3420

3361 gagttcagtc tccaccgctt tgggctgtcc tgatatttct atggtccgca aagggggacc 3420

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3421 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 3480

3421 ttcgagggag cacgcgagag gacaaggctg ggacggcgaa tggcctatgg acaggcggaa 3480

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3481 tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc tcagttcggt 3540

3481 agagggaagc ccttcgcacc gcgaaagagt tacgagtgcg acatccatag agtcaagcca 3540

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3541 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 3600

3541 catccagcaa gcgaggttcg acccgacaca cgtgcttggg gggcaagtcg ggctggcgac 3600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3601 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 3660

3601 gcggaatagg ccattgatag cagaactcag gttgggccat tctgtgctga atagcggtga 3660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 3720

3661 ccgtcgtcgg tgaccattgt cctaatcgtc tcgctccata catccgccac gatgtctcaa 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct 3780

3721 gaacttcacc accggattga tgccgatgtg atcttcctgt cataaaccat agacgcgaga 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 3840

3781 cgacttcggt caatggaagc ctttttctca accatcgaga actaggccgt ttgtttggtg 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 3900

3841 gcgaccatcg ccaccaaaaa aacaaacgtt cgtcgtctaa tgcgcgtctt tttttcctag 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 3960

3901 agttcttcta ggaaactaga aaagatgccc cagactgcga gtcaccttgc ttttgagtgc 3960

pBR322_origin ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 4020

3961 aattccctaa aaccagtact ctaatagttt ttcctagaag tggatctagg aaaatttaat 4020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 4080

4021 ttttacttca aaatttagtt agatttcata tatactcatt tgaaccagac tgtcaatggt 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 4140

4081 tacgaattag tcactccgtg gatagagtcg ctagacagat aaagcaagta ggtatcaacg 4140

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 4200

4141 gactgagggg cagcacatct attgatgcta tgccctcccg aatggtagac cggggtcacg 4200

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 4260

4201 acgttactat ggcgctctgg gtgcgagtgg ccgaggtcta aatagtcgtt atttggtcgg 4260

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 4320

4261 tcggccttcc cggctcgcgt cttcaccagg acgttgaaat aggcggaggt aggtcagata 4320

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... FspI(4370) ..........

4321 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 4380

4321 attaacaacg gcccttcgat ctcattcatc aagcggtcaa ttatcaaacg cgttgcaaca 4380

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 4440

4381 acggtaacga tgtccgtagc accacagtgc gagcagcaaa ccataccgaa gtaagtcgag 4440

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 4500

4441 gccaagggtt gctagttccg ctcaatgtac tagggggtac aacacgtttt ttcgccaatc 4500

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 4560

4501 gaggaagcca ggaggctagc aacagtcttc attcaaccgg cgtcacaata gtgagtacca 4560

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 4620

4561 ataccgtcgt gacgtattaa gagaatgaca gtacggtagg cattctacga aaagacactg 4620

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 4680

4621 accactcatg agttggttca gtaagactct tatcacatac gccgctggct caacgagaac 4680

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 4740

4681 gggccgcagt tatgccctat tatggcgcgg tgtatcgtct tgaaattttc acgagtagta 4740

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 4800

4741 accttttgca agaagccccg cttttgagag ttcctagaat ggcgacaact ctaggtcaag 4800

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 4860

4801 ctacattggg tgagcacgtg ggttgactag aagtcgtaga aaatgaaagt ggtcgcaaag 4860

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 4920

4861 acccactcgt ttttgtcctt ccgttttacg gcgttttttc ccttattccc gctgtgcctt 4920

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg 4980

4921 tacaacttat gagtatgaga aggaaaaagt tataataact tcgtaaatag tcccaataac 4980

ORF 1 Ampicillin .... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 5040

4981 agagtactcg cctatgtata aacttacata aatcttttta tttgtttatc cccaaggcgc 5040

.......... AmpR_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 cacatttccc cgaaaagtgc cacctgacgt c......... .......... .......... 5071

5041 gtgtaaaggg gcttttcacg gtggactgca g......... .......... .......... 5071

.......... .......... .......... .......... .......... ..........