Sequence of pcDNA5_FRT

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Sequence of pcDNA5_FRT_TO-E

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Sequence of pcDNA5/FRT/TO-E : 4870 BP; 1141 A; 1263 C; 1243 G; 1223 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 60

1 ctgcctagcc ctctagaggg ctaggggata ccacgtgaga gtcatgttag acgagactac 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120

61 ggcgtatcaa ttcggtcata gacgagggac gaacacacaa cctccagcga ctcatcacgc 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180

121 gctcgtttta aattcgatgt tgttccgttc cgaactggct gttaacgtac ttcttagacg 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240

181 aatcccaatc cgcaaaacgc gacgaagcgc tacatgcccg gtctatatgc gcaactgtaa 240

.......... .......... .......... .......... .......... ..........

SpeI(249) .......... .......... .......... .......... ..........

241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 300

241 ctaataactg atcaataatt atcattagtt aatgccccag taatcaagta tcgggtatat 300

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 360

301 acctcaaggc gcaatgtatt gaatgccatt taccgggcgg accgactggc gggttgctgg 360

.......... ....... CMV_immearly_pro CAG_enh ....... ..........

.......... .......... .......... .......... .......... ..........

361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 420

361 gggcgggtaa ctgcagttat tactgcatac aagggtatca ttgcggttat ccctgaaagg 420

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 480

421 taactgcagt tacccacctc ataaatgcca tttgacgggt gaaccgtcat gtagttcaca 480

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 540

481 tagtatacgg ttcatgcggg ggataactgc agttactgcc atttaccggg cggaccgtaa 540

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 600

541 tacgggtcat gtactggaat accctgaaag gatgaaccgt catgtagatg cataatcagt 600

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 660

601 agcgataatg gtaccactac gccaaaaccg tcatgtagtt acccgcacct atcgccaaac 660

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 720

661 tgagtgcccc taaaggttca gaggtggggt aactgcagtt accctcaaac aaaaccgtgg 720

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 780

721 ttttagttgc cctgaaaggt tttacagcat tgttgaggcg gggtaactgc gtttacccgc 780

.......... ....... CMV_immearly_pro ....... ..... CMV_fwd_pri CMV_pro

.......... .......... .......... .......... .......... ..........

781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc 840

781 catccgcaca tgccaccctc cagatatatt cgtctcgaga gggatagtca ctatctctag 840

CMV_fwd_pri CMV_immearly_pro CMV_pro CMV2_pro tetO ..

.......... .......... .......... .......... .......... ..........

841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga 900

841 agggatagtc actatctcta gcagctgctc gagcaaatca cttggcagtc tagcggacct 900

tetO ....... CMV_pro CMV2_pro ....... LNCX_pri ..........

.......... .......... .......... .......... .......... ..........

901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga 960

901 ctgcggtagg tgcgacaaaa ctggaggtat cttctgtggc cctggctagg tcggaggcct 960

.......... .......... CMV2_pro .......... .......... ..........

... AflII(975),HindIII(978) KpnI(988) ... .......... ..........

961 ctctagcgtt taaacttaag cttggtacca taacttcgta tagcatacat tatacgaagt 1020

961 gagatcgcaa atttgaattc gaaccatggt attgaagcat atcgtatgta atatgcttca 1020

.......... .......... .......... .......... loxP ..........

.......... .......... .......... .......... .......... ..........

1021 tatgcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg 1080

1021 atacgtacga cccctacgcc acccgagata ccgaagactc cgcctttctt ggtcgacccc 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt 1140

1081 gagatccccc ataggggtgc gcgggacatc gccgcgtaat tcgcgccgcc cacaccacca 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt 1200

1141 atgcgcgtcg cactggcgat gtgaacggtc gcgggatcgc gggcgaggaa agcgaaagaa 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc 1260

1201 gggaaggaaa gagcggtgca agcggccgaa aggggcagtt cgagatttag cccccgaggg 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga 1320

1261 aaatcccaag gctaaatcac gaaatgccgt ggagctgggg ttttttgaac taatcccact 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 tggttcacgt acctagaagt tcctattccg aagttcctat tctctagaaa gtataggaac 1380

1321 accaagtgca tggatcttca aggataaggc ttcaaggata agagatcttt catatccttg 1380

.......... .......... .......... FRT .......... ORF 2

MscI(1388) .......... .......... .......... .......... ..........

1381 ttccttggcc aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa 1440

1381 aaggaaccgg tttttcggac ttgagtggcg ctgcagacag ctcttcaaag actagctttt 1440

.......... .......... ORF 2 .......... hygroB ..........

.......... .......... .......... .......... .......... ..........

1441 gttcgacagc gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag 1500

1441 caagctgtcg cagaggctgg actacgtcga gagcctcccg cttcttagag cacgaaagtc 1500

.......... ......... ORF 2 hygroB ......... ORF 2 ..........

.......... .......... .......... .......... .......... ..........

1501 cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta 1560

1501 gaagctacat cctcccgcac ctatacagga cgcccattta tcgacgcggc taccaaagat 1560

.......... ...... ORF 2 hygroB ORF 2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 caaagatcgt tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct 1620

1561 gtttctagca atacaaatag ccgtgaaacg tagccggcgc gagggctaag gccttcacga 1620

.......... ...... ORF 2 hygroB ORF 2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 tgacattggg gaattcagcg agagcctgac ctattgcatc tcccgccgtg cacagggtgt 1680

1621 actgtaaccc cttaagtcgc tctcggactg gataacgtag agggcggcac gtgtcccaca 1680

.......... ...... ORF 2 hygroB ORF 2 ...... .......... ..........

.......... .......... .......... .......... PstI(1725) ..........

1681 cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc 1740

1681 gtgcaacgtt ctggacggac tttggcttga cgggcgacaa gacgtcggcc agcgcctccg 1740

.......... ...... ORF 2 hygroB ORF 2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 catggatgcg atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc 1800

1741 gtacctacgc tagcgacgcc ggctagaatc ggtctgctcg cccaagccgg gtaagcctgg 1800

.......... ...... ORF 2 hygroB ORF 2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 gcaaggaatc ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca 1860

1801 cgttccttag ccagttatgt gatgtaccgc actaaagtat acgcgctaac gactaggggt 1860

.......... ...... ORF 2 hygroB ORF 2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 tgtgtatcac tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct 1920

1861 acacatagtg accgtttgac actacctgct gtggcagtca cgcaggcagc gcgtccgaga 1920

.......... ...... ORF 2 hygroB ORF 2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 cgatgagctg atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga 1980

1921 gctactcgac tacgaaaccc ggctcctgac ggggcttcag gccgtggagc acgtgcgcct 1980

.......... ...... ORF 2 hygroB ORF 2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 tttcggctcc aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag 2040

1981 aaagccgagg ttgttacagg actgcctgtt accggcgtat tgtcgccagt aactgacctc 2040

.......... ...... ORF 2 hygroB ORF 2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 cgaggcgatg ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg 2100

2041 gctccgctac aagcccctaa gggttatgct ccagcggttg tagaagaaga cctccggcac 2100

.......... ...... ORF 2 hygroB ORF 2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 gttggcttgt atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg 2160

2101 caaccgaaca tacctcgtcg tctgcgcgat gaagctcgcc tccgtaggcc tcgaacgtcc 2160

.......... ...... ORF 2 hygroB ORF 2 ...... .......... ..........

SacII(2168) ........ .......... .......... .......... ..........

2161 atcgccgcgg ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt 2220

2161 tagcggcgcc gaggcccgca tatacgaggc gtaaccagaa ctggttgaga tagtctcgaa 2220

.......... ......... hygroB ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 ggttgacggc aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg 2280

2221 ccaactgccg ttaaagctac tacgtcgaac ccgcgtccca gctacgctgc gttagcaggc 2280

.......... ......... hygroB ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 atccggagcc gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac 2340

2281 taggcctcgg ccctgacagc ccgcatgtgt ttagcgggcg tcttcgcgcc ggcagacctg 2340

.......... ......... hygroB ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 cgatggctgt gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgag 2400

2341 gctaccgaca catcttcatg agcggctatc acctttggct gcggggtcgt gagcaggctc 2400

.......... ......... hygroB ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 ggcaaaggaa tagcacgtac tacgagattt cgattccacc gccgccttct atgaaaggtt 2460

2401 ccgtttcctt atcgtgcatg atgctctaaa gctaaggtgg cggcggaaga tactttccaa 2460

hygroB ORF 2 ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat 2520

2461 cccgaagcct tagcaaaagg ccctgcggcc gacctactag gaggtcgcgc ccctagagta 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag 2580

2521 cgacctcaag aagcgggtgg ggttgaacaa ataacgtcga atattaccaa tgtttatttc 2580

.......... .......... .......... ......... SV40_PA_term .........

.......... .......... .......... .......... .......... ..........

2581 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt 2640

2581 gttatcgtag tgtttaaagt gtttatttcg taaaaaaagt gacgtaagat caacaccaaa 2640

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt 2700

2641 caggtttgag tagttacata gaatagtaca gacatatggc agctggagat cgatctcgaa 2700

EBV_rev_pri SV40_PA_term ...... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca 2760

2701 ccgcattagt accagtatcg acaaaggaca cactttaaca ataggcgagt gttaaggtgt 2760

....... M13_reverse_pri .... M13_pUC_rev_pri ....... ..........

.......... .......... .......... .......... .......... ..........

2761 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact 2820

2761 gttgtatgct cggccttcgt atttcacatt tcggacccca cggattactc actcgattga 2820

.......... lac_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct 2880

2821 gtgtaattaa cgcaacgcga gtgacgggcg aaaggtcagc cctttggaca gcacggtcga 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc 2940

2881 cgtaattact tagccggttg cgcgcccctc tccgccaaac gcataacccg cgagaaggcg 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 3000

2941 aaggagcgag tgactgagcg acgcgagcca gcaagccgac gccgctcgcc atagtcgagt 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 3060

3001 gagtttccgc cattatgcca ataggtgtct tagtccccta ttgcgtcctt tcttgtacac 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 3120

3061 tcgttttccg gtcgttttcc ggtccttggc atttttccgg cgcaacgacc gcaaaaaggt 3120

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

3121 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 3180

3121 atccgaggcg gggggactgc tcgtagtgtt tttagctgcg agttcagtct ccaccgcttt 3180

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3181 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 3240

3181 gggctgtcct gatatttcta tggtccgcaa agggggacct tcgagggagc acgcgagagg 3240

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3241 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 3300

3241 acaaggctgg gacggcgaat ggcctatgga caggcggaaa gagggaagcc cttcgcaccg 3300

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3301 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 3360

3301 cgaaagagta tcgagtgcga catccataga gtcaagccac atccagcaag cgaggttcga 3360

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3361 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 3420

3361 cccgacacac gtgcttgggg ggcaagtcgg gctggcgacg cggaataggc cattgatagc 3420

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3421 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 3480

3421 agaactcagg ttgggccatt ctgtgctgaa tagcggtgac cgtcgtcggt gaccattgtc 3480

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3481 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 3540

3481 ctaatcgtct cgctccatac atccgccacg atgtctcaag aacttcacca ccggattgat 3540

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3541 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 3600

3541 gccgatgtga tcttcttgtc ataaaccata gacgcgagac gacttcggtc aatggaagcc 3600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3601 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gggttttttt 3660

3601 tttttctcaa ccatcgagaa ctaggccgtt tgtttggtgg cgaccatcgc cccaaaaaaa 3660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 3720

3661 acaaacgttc gtcgtctaat gcgcgtcttt ttttcctaga gttcttctag gaaactagaa 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 3780

3721 aagatgcccc agactgcgag tcaccttgct tttgagtgca attccctaaa accagtactc 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 3840

3781 taatagtttt tcctagaagt ggatctagga aaatttaatt tttacttcaa aatttagtta 3840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 3900

3841 gatttcatat atactcattt gaaccagact gtcaatggtt acgaattagt cactccgtgg 3900

.......... .......... .......... ....... ORF 1 Ampicillin .......

.......... .......... .......... .......... .......... ..........

3901 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 3960

3901 atagagtcgc tagacagata aagcaagtag gtatcaacgg actgaggggc agcacatcta 3960

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 4020

3961 ttgatgctat gccctcccga atggtagacc ggggtcacga cgttactatg gcgctctggg 4020

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 4080

4021 tgcgagtggc cgaggtctaa atagtcgtta tttggtcggt cggccttccc ggctcgcgtc 4080

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 4140

4081 ttcaccagga cgttgaaata ggcggaggta ggtcagataa ttaacaacgg cccttcgatc 4140

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... FspI(4169) .......... .......... ..........

4141 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 4200

4141 tcattcatca agcggtcaat tatcaaacgc gttgcaacaa cggtaacgat gtccgtagca 4200

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 4260

4201 ccacagtgcg agcagcaaac cataccgaag taagtcgagg ccaagggttg ctagttccgc 4260

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 4320

4261 tcaatgtact agggggtaca acacgttttt tcgccaatcg aggaagccag gaggctagca 4320

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 4380

4321 acagtcttca ttcaaccggc gtcacaatag tgagtaccaa taccgtcgtg acgtattaag 4380

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 4440

4381 agaatgacag tacggtaggc attctacgaa aagacactga ccactcatga gttggttcag 4440

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 4500

4441 taagactctt atcacatacg ccgctggctc aacgagaacg ggccgcagtt atgccctatt 4500

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 4560

4501 atggcgcggt gtatcgtctt gaaattttca cgagtagtaa ccttttgcaa gaagccccgc 4560

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 4620

4561 ttttgagagt tcctagaatg gcgacaactc taggtcaagc tacattgggt gagcacgtgg 4620

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 4680

4621 gttgactaga agtcgtagaa aatgaaagtg gtcgcaaaga cccactcgtt tttgtccttc 4680

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 4740

4681 cgttttacgg cgttttttcc cttattcccg ctgtgccttt acaacttatg agtatgagaa 4740

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 4800

4741 ggaaaaagtt ataataactt cgtaaatagt cccaataaca gagtactcgc ctatgtataa 4800

.......... .......... .......... .......... AmpR_pro ..........

.......... .......... .......... .......... .......... ..........

4801 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 4860

4801 acttacataa atctttttat ttgtttatcc ccaaggcgcg tgtaaagggg cttttcacgg 4860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 acctgacgtc .......... .......... .......... .......... .......... 4870

4861 tggactgcag .......... .......... .......... .......... .......... 4870

.......... .......... .......... .......... .......... ..........