Sequence of pcDNA5_FRT

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Sequence of pcDNA5_FRT_TO-TOPO

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Sequence of pcDNA5/FRT/TO-TOPO : 5155 BP; 1191 A; 1342 C; 1334 G; 1288 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 60

1 ctgcctagcc ctctagaggg ctaggggata ccacgtgaga gtcatgttag acgagactac 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120

61 ggcgtatcaa ttcggtcata gacgagggac gaacacacaa cctccagcga ctcatcacgc 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180

121 gctcgtttta aattcgatgt tgttccgttc cgaactggct gttaacgtac ttcttagacg 180

.......... .......... .......... .......... .......... ..........

.......... .......... NruI(208) .......... .......... ..........

181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240

181 aatcccaatc cgcaaaacgc gacgaagcgc tacatgcccg gtctatatgc gcaactgtaa 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 300

241 ctaataactg atcaataatt atcattagtt aatgccccag taatcaagta tcgggtatat 300

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 360

301 acctcaaggc gcaatgtatt gaatgccatt taccgggcgg accgactggc gggttgctgg 360

.......... ....... CMV_immearly_pro CAG_enh ....... ..........

.......... .......... .......... .......... .......... ..........

361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 420

361 gggcgggtaa ctgcagttat tactgcatac aagggtatca ttgcggttat ccctgaaagg 420

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 480

421 taactgcagt tacccacctc ataaatgcca tttgacgggt gaaccgtcat gtagttcaca 480

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 540

481 tagtatacgg ttcatgcggg ggataactgc agttactgcc atttaccggg cggaccgtaa 540

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 600

541 tacgggtcat gtactggaat accctgaaag gatgaaccgt catgtagatg cataatcagt 600

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 660

601 agcgataatg gtaccactac gccaaaaccg tcatgtagtt acccgcacct atcgccaaac 660

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 720

661 tgagtgcccc taaaggttca gaggtggggt aactgcagtt accctcaaac aaaaccgtgg 720

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 780

721 ttttagttgc cctgaaaggt tttacagcat tgttgaggcg gggtaactgc gtttacccgc 780

.......... ....... CMV_immearly_pro ....... ..... CMV_fwd_pri CMV_pro

.......... .......... .......... .......... .......... ..........

781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc 840

781 catccgcaca tgccaccctc cagatatatt cgtctcgaga gggatagtca ctatctctag 840

CMV_fwd_pri CMV_immearly_pro CMV_pro CMV2_pro tetO ..

.......... .......... .......... .......... .......... ..........

841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga 900

841 agggatagtc actatctcta gcagctgctc gagcaaatca cttggcagtc tagcggacct 900

tetO ....... CMV_pro CMV2_pro ....... LNCX_pri ..........

.......... .......... .......... .......... .......... ..........

901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga 960

901 ctgcggtagg tgcgacaaaa ctggaggtat cttctgtggc cctggctagg tcggaggcct 960

.......... .......... CMV2_pro .......... .......... ..........

.......... .......... .......... .......... KpnI(1006) ..........

961 ctctagcgtt taaacttaag ctcgccctta agggcgagct tggtaccgag ctcggatcca 1020

961 gagatcgcaa atttgaattc gagcgggaat tcccgctcga accatggctc gagcctaggt 1020

.......... .......... .......... .......... .......... ..........

.......... ......... EcoRV(1049) ......... NotI(1064) ..........

1021 ctagtccagt gtggtggaat tctgcagata tccagcacag tggcggccgc tcgagtctag 1080

1021 gatcaggtca caccacctta agacgtctat aggtcgtgtc accgccggcg agctcagatc 1080

.......... .......... .......... .......... .......... ..........

ApaI(1086) BclI(1100) .......... .......... .......... ..........

1081 agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt 1140

1081 tcccgggcaa atttgggcga ctagtcggag ctgacacgga agatcaacgg tcggtagaca 1140

.......... .......... ........ BGH_rev_pri bGH_PA_term ........

.......... .......... .......... .......... .......... ..........

1141 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc 1200

1141 acaaacgggg agggggcacg gaaggaactg ggaccttcca cggtgagggt gacaggaaag 1200

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg 1260

1201 gattatttta ctcctttaac gtagcgtaac agactcatcc acagtaagat aagacccccc 1260

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga 1320

1261 accccacccc gtcctgtcgt tccccctcct aacccttctg ttatcgtccg tacgacccct 1320

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc 1380

1321 acgccacccg agataccgaa gactccgcct ttcttggtcg accccgagat cccccatagg 1380

bGH_PA_term ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac 1440

1381 ggtgcgcggg acatcgccgc gtaattcgcg ccgcccacac caccaatgcg cgtcgcactg 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc 1500

1441 gcgatgtgaa cggtcgcggg atcgcgggcg aggaaagcga aagaagggaa ggaaagagcg 1500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt 1560

1501 gtgcaagcgg ccgaaagggg cagttcgaga tttagccccc gagggaaatc ccaaggctaa 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtaccta 1620

1561 atcacgaaat gccgtggagc tggggttttt tgaactaatc ccactaccaa gtgcatggat 1620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... MscI(1673)

1621 gaagttccta ttccgaagtt cctattctct agaaagtata ggaacttcct tggccaaaaa 1680

1621 cttcaaggat aaggcttcaa ggataagaga tctttcatat ccttgaagga accggttttt 1680

.......... .......... FRT .......... ORF 2 ..........

.......... .......... .......... .......... .......... ..........

1681 gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc 1740

1681 cggacttgag tggcgctgca gacagctctt caaagactag cttttcaagc tgtcgcagag 1740

.......... .......... ORF 2 hygroB .......... ..........

.......... .......... .......... .......... .......... ..........

1741 cgacctgatg cagctctcgg agggcgaaga atctcgtgct ttcagcttcg atgtaggagg 1800

1741 gctggactac gtcgagagcc tcccgcttct tagagcacga aagtcgaagc tacatcctcc 1800

.......... ......... ORF 2 hygroB ORF 2 ......... ..........

.......... .......... .......... .......... .......... ..........

1801 gcgtggatat gtcctgcggg taaatagctg cgccgatggt ttctacaaag atcgttatgt 1860

1801 cgcacctata caggacgccc atttatcgac gcggctacca aagatgtttc tagcaataca 1860

.......... ...... ORF 2 hygroB ORF 2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 ttatcggcac tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca ttggggaatt 1920

1861 aatagccgtg aaacgtagcc ggcgcgaggg ctaaggcctt cacgaactgt aaccccttaa 1920

.......... ...... ORF 2 hygroB ORF 2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 cagcgagagc ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct 1980

1921 gtcgctctcg gactggataa cgtagagggc ggcacgtgtc ccacagtgca acgttctgga 1980

.......... ...... ORF 2 hygroB ORF 2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc 2040

1981 cggactttgg cttgacgggc gacaagacgt cggccagcgc ctccggtacc tacgctagcg 2040

.......... ...... ORF 2 hygroB ORF 2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tgcggccgat cttagccaga cgagcgggtt cggcccattc ggaccgcaag gaatcggtca 2100

2041 acgccggcta gaatcggtct gctcgcccaa gccgggtaag cctggcgttc cttagccagt 2100

.......... ...... ORF 2 hygroB ORF 2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 atacactaca tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt atcactggca 2160

2101 tatgtgatgt accgcactaa agtatacgcg ctaacgacta ggggtacaca tagtgaccgt 2160

.......... ...... ORF 2 hygroB ORF 2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct 2220

2161 ttgacactac ctgctgtggc agtcacgcag gcagcgcgtc cgagagctac tcgactacga 2220

.......... ...... ORF 2 hygroB ORF 2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 ttgggccgag gactgccccg aagtccggca cctcgtgcac gcggatttcg gctccaacaa 2280

2221 aacccggctc ctgacggggc ttcaggccgt ggagcacgtg cgcctaaagc cgaggttgtt 2280

.......... ...... ORF 2 hygroB ORF 2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 tgtcctgacg gacaatggcc gcataacagc ggtcattgac tggagcgagg cgatgttcgg 2340

2281 acaggactgc ctgttaccgg cgtattgtcg ccagtaactg acctcgctcc gctacaagcc 2340

.......... ...... ORF 2 hygroB ORF 2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 ggattcccaa tacgaggtcg ccaacatctt cttctggagg ccgtggttgg cttgtatgga 2400

2341 cctaagggtt atgctccagc ggttgtagaa gaagacctcc ggcaccaacc gaacatacct 2400

.......... ...... ORF 2 hygroB ORF 2 ...... .......... ..........

.......... .......... .......... .......... ......... SacII(2453)

2401 gcagcagacg cgctacttcg agcggaggca tccggagctt gcaggatcgc cgcggctccg 2460

2401 cgtcgtctgc gcgatgaagc tcgcctccgt aggcctcgaa cgtcctagcg gcgccgaggc 2460

......... ORF 2 hygroB ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ggcgtatatg ctccgcattg gtcttgacca actctatcag agcttggttg acggcaattt 2520

2461 ccgcatatac gaggcgtaac cagaactggt tgagatagtc tcgaaccaac tgccgttaaa 2520

.......... ......... hygroB ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg gagccgggac 2580

2521 gctactacgt cgaacccgcg tcccagctac gctgcgttag caggctaggc ctcggccctg 2580

.......... ......... hygroB ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga 2640

2581 acagcccgca tgtgtttagc gggcgtcttc gcgccggcag acctggctac cgacacatct 2640

.......... ......... hygroB ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 agtactcgcc gatagtggaa accgacgccc cagcactcgt ccgagggcaa aggaatagca 2700

2641 tcatgagcgg ctatcacctt tggctgcggg gtcgtgagca ggctcccgtt tccttatcgt 2700

.......... ......... hygroB ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 cgtactacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt 2760

2701 gcatgatgct ctaaagctaa ggtggcggcg gaagatactt tccaacccga agccttagca 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc 2820

2761 aaaggccctg cggccgacct actaggaggt cgcgccccta gagtacgacc tcaagaagcg 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa 2880

2821 ggtggggttg aacaaataac gtcgaatatt accaatgttt atttcgttat cgtagtgttt 2880

.......... .......... ......... SV40_PA_term ......... ..........

.......... .......... .......... .......... .......... ..........

2881 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca a actcatcaa 2940

2881 aaagtgttta tttcgtaaaa aaagtgacgt aagatcaaca ccaaacaggt ttgagtagtt 2940

.......... ......... SV40_PA_term ........ EBV_rev_pri .........

.......... .......... .......... .......... .......... ..........

2941 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc 3000

2941 acatagaata gtacagacat atggcagctg gagatcgatc tcgaaccgca ttagtaccag 3000

SV40_PA_term ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg 3060

3001 tatcgacaaa ggacacactt taacaatagg cgagtgttaa ggtgtgttgt atgctcggcc 3060

M13_reverse_pri . M13_pUC_rev_pri ....... .......... lac_pro

.......... .......... .......... .......... .......... ..........

3061 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt 3120

3061 ttcgtatttc acatttcgga ccccacggat tactcactcg attgagtgta attaacgcaa 3120

lac_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg 3180

3121 cgcgagtgac gggcgaaagg tcagcccttt ggacagcacg gtcgacgtaa ttacttagcc 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga 3240

3181 ggttgcgcgc ccctctccgc caaacgcata acccgcgaga aggcgaagga gcgagtgact 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 3300

3241 gagcgacgcg agccagcaag ccgacgccgc tcgccatagt cgagtgagtt tccgccatta 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 3360

3301 tgccaatagg tgtcttagtc ccctattgcg tcctttcttg tacactcgtt ttccggtcgt 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 3420

3361 tttccggtcc ttggcatttt tccggcgcaa cgaccgcaaa aaggtatccg aggcgggggg 3420

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

3421 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata 3480

3421 actgctcgta gtgtttttag ctgcgagttc agtctccacc gctttgggct gtcctgatat 3480

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3481 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 3540

3481 ttctatggtc cgcaaagggg gaccttcgag ggagcacgcg agaggacaag gctgggacgg 3540

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3541 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc 3600

3541 cgaatggcct atggacaggc ggaaagaggg aagcccttcg caccgcgaaa gagtatcgag 3600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3601 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga 3660

3601 tgcgacatcc atagagtcaa gccacatcca gcaagcgagg ttcgacccga cacacgtgct 3660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 3720

3661 tggggggcaa gtcgggctgg cgacgcggaa taggccattg atagcagaac tcaggttggg 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag 3780

3721 ccattctgtg ctgaatagcg gtgaccgtcg tcggtgacca ttgtcctaat cgtctcgctc 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag 3840

3781 catacatccg ccacgatgtc tcaagaactt caccaccgga ttgatgccga tgtgatcttc 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag 3900

3841 ttgtcataaa ccatagacgc gagacgactt cggtcaatgg aagccttttt ctcaaccatc 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca 3960

3901 gagaactagg ccgtttgttt ggtggcgacc atcgccacca aaaaaacaaa cgttcgtcgt 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga 4020

3961 ctaatgcgcg tctttttttc ctagagttct tctaggaaac tagaaaagat gccccagact 4020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat 4080

4021 gcgagtcacc ttgcttttga gtgcaattcc ctaaaaccag tactctaata gtttttccta 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga 4140

4081 gaagtggatc taggaaaatt taatttttac ttcaaaattt agttagattt catatatact 4140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg 4200

4141 catttgaacc agactgtcaa tggttacgaa ttagtcactc cgtggataga gtcgctagac 4200

.......... .......... ....... ORF 1 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

4201 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga 4260

4201 agataaagca agtaggtatc aacggactga ggggcagcac atctattgat gctatgccct 4260

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc 4320

4261 cccgaatggt agaccggggt cacgacgtta ctatggcgct ctgggtgcga gtggccgagg 4320

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac 4380

4321 tctaaatagt cgttatttgg tcggtcggcc ttcccggctc gcgtcttcac caggacgttg 4380

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc 4440

4381 aaataggcgg aggtaggtca gataattaac aacggccctt cgatctcatt catcaagcgg 4440

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... FspI(4454) .......... .......... .......... ..........

4441 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc 4500

4441 tcaattatca aacgcgttgc aacaacggta acgatgtccg tagcaccaca gtgcgagcag 4500

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc 4560

4501 caaaccatac cgaagtaagt cgaggccaag ggttgctagt tccgctcaat gtactagggg 4560

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt 4620

4561 gtacaacacg ttttttcgcc aatcgaggaa gccaggaggc tagcaacagt cttcattcaa 4620

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc 4680

4621 ccggcgtcac aatagtgagt accaataccg tcgtgacgta ttaagagaat gacagtacgg 4680

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg 4740

4681 taggcattct acgaaaagac actgaccact catgagttgg ttcagtaaga ctcttatcac 4740

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag 4800

4741 atacgccgct ggctcaacga gaacgggccg cagttatgcc ctattatggc gcggtgtatc 4800

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat 4860

4801 gtcttgaaat tttcacgagt agtaaccttt tgcaagaagc cccgcttttg agagttccta 4860

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc 4920

4861 gaatggcgac aactctaggt caagctacat tgggtgagca cgtgggttga ctagaagtcg 4920

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa 4980

4921 tagaaaatga aagtggtcgc aaagacccac tcgtttttgt ccttccgttt tacggcgttt 4980

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta 5040

4981 tttcccttat tcccgctgtg cctttacaac ttatgagtat gagaaggaaa aagttataat 5040

....... ORF 1 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa 5100

5041 aacttcgtaa atagtcccaa taacagagta ctcgcctatg tataaactta cataaatctt 5100

.......... .......... .......... AmpR_pro .......... ..........

.......... .......... .......... .......... .......... ..........

5101 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtc..... 5155

5101 tttatttgtt tatccccaag gcgcgtgtaa aggggctttt cacggtggac tgcag..... 5155

.......... .......... .......... .......... .......... ..........