OXOX'LOCUS pcDNA5/FRT/TO-TOPO 5155 bp DNA SYN 11-Sep-2007 DEFINITION pcDNA5/FRT/TO-TOPO ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..5155 /organism="pcDNA5/FRT/TO-TOPO" /mol_type="other DNA" misc_feature 315..602 /label="CAG_enhancer" promoter 236..812 /label="CMV_immearly_promoter" misc_feature 769..789 /label="CMV_fwd_primer" promoter 770..893 /label="CMV_promoter" misc_feature 820..859 /label="tetO" promoter 782..955 /label="CMV2_promoter" misc_feature 869..893 /label="LNCX_primer" misc_feature complement(1107..1124) /label="BGH_rev_primer" terminator 1110..1337 /label="bGH_PA_terminator" misc_feature 1621..1668 /label="FRT" CDS complement(1649..2431) /label="ORF frame 2" /translation="MPPLEVARLLLHTSQPRPPEEDVGDLVLGIPEHRLAPVNDRCYA AIVRQDIVGAEIRVHEVPDFGAVLGPKHQLIESLRDGRTDGVVHHSLPVIHMGISNRA YEITPCSVLTDSLRSEWAEPARLAKIGRSDRIHGLRDRLQNSGQFGFRQVLQRDTLCT AGDAIGQALAEFPNVKHFRNRERGRCKVPINITIFVETIGAAIYPQDISTPSYIEAES TRFFALRELHQVGDAVELFDQKLLDRRRGEFRLFGQGSSYTF*" gene 1688..2695 /label="hygroB" /gene="hygroB" CDS 1748..2698 /label="ORF frame 2" /translation="MQLSEGEESRAFSFDVGGRGYVLRVNSCADGFYKDRYVYRHFAS AALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPETELPAVLQPVAEAMDAIAAADL SQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTVMDDTVSASVAQALDELMLWA EDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGDSQYEVANIFFWRPWLACME QQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGA GTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE*" terminator 2831..2950 /label="SV40_PA_terminator" misc_feature 2919..2938 /label="EBV_rev_primer" misc_feature complement(2994..3012) /label="M13_reverse_primer" misc_feature complement(3011..3033) /label="M13_pUC_rev_primer" promoter complement(3047..3076) /label="lac_promoter" rep_origin complement(3385..4004) /label="pBR322_origin" CDS complement(4159..5019) /label="Ampicillin" /gene="Ampicillin" /note="ORF frame 1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" gene complement(4159..5019) /label="Ampicillin" /gene="Ampicillin" promoter complement(5061..5089) /label="AmpR_promoter" ORIGIN 1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG 61 CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG 121 CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC 181 TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT 241 GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA 301 TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC 361 CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC 421 ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT 481 ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT 541 ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA 601 TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG 661 ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC 721 AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG 781 GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CCCTATCAGT GATAGAGATC 841 TCCCTATCAG TGATAGAGAT CGTCGACGAG CTCGTTTAGT GAACCGTCAG ATCGCCTGGA 901 GACGCCATCC ACGCTGTTTT GACCTCCATA GAAGACACCG GGACCGATCC AGCCTCCGGA 961 CTCTAGCGTT TAAACTTAAG CTCGCCCTTA AGGGCGAGCT TGGTACCGAG CTCGGATCCA 1021 CTAGTCCAGT GTGGTGGAAT TCTGCAGATA TCCAGCACAG TGGCGGCCGC TCGAGTCTAG 1081 AGGGCCCGTT TAAACCCGCT GATCAGCCTC GACTGTGCCT TCTAGTTGCC AGCCATCTGT 1141 TGTTTGCCCC TCCCCCGTGC CTTCCTTGAC CCTGGAAGGT GCCACTCCCA CTGTCCTTTC 1201 CTAATAAAAT GAGGAAATTG CATCGCATTG TCTGAGTAGG TGTCATTCTA TTCTGGGGGG 1261 TGGGGTGGGG CAGGACAGCA AGGGGGAGGA TTGGGAAGAC AATAGCAGGC ATGCTGGGGA 1321 TGCGGTGGGC TCTATGGCTT CTGAGGCGGA AAGAACCAGC TGGGGCTCTA GGGGGTATCC 1381 CCACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC 1441 CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC 1501 CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT 1561 TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTACCTA 1621 GAAGTTCCTA TTCCGAAGTT CCTATTCTCT AGAAAGTATA GGAACTTCCT TGGCCAAAAA 1681 GCCTGAACTC ACCGCGACGT CTGTCGAGAA GTTTCTGATC GAAAAGTTCG ACAGCGTCTC 1741 CGACCTGATG CAGCTCTCGG AGGGCGAAGA ATCTCGTGCT TTCAGCTTCG ATGTAGGAGG 1801 GCGTGGATAT GTCCTGCGGG TAAATAGCTG CGCCGATGGT TTCTACAAAG ATCGTTATGT 1861 TTATCGGCAC TTTGCATCGG CCGCGCTCCC GATTCCGGAA GTGCTTGACA TTGGGGAATT 1921 CAGCGAGAGC CTGACCTATT GCATCTCCCG CCGTGCACAG GGTGTCACGT TGCAAGACCT 1981 GCCTGAAACC GAACTGCCCG CTGTTCTGCA GCCGGTCGCG GAGGCCATGG ATGCGATCGC 2041 TGCGGCCGAT CTTAGCCAGA CGAGCGGGTT CGGCCCATTC GGACCGCAAG GAATCGGTCA 2101 ATACACTACA TGGCGTGATT TCATATGCGC GATTGCTGAT CCCCATGTGT ATCACTGGCA 2161 AACTGTGATG GACGACACCG TCAGTGCGTC CGTCGCGCAG GCTCTCGATG AGCTGATGCT 2221 TTGGGCCGAG GACTGCCCCG AAGTCCGGCA CCTCGTGCAC GCGGATTTCG GCTCCAACAA 2281 TGTCCTGACG GACAATGGCC GCATAACAGC GGTCATTGAC TGGAGCGAGG CGATGTTCGG 2341 GGATTCCCAA TACGAGGTCG CCAACATCTT CTTCTGGAGG CCGTGGTTGG CTTGTATGGA 2401 GCAGCAGACG CGCTACTTCG AGCGGAGGCA TCCGGAGCTT GCAGGATCGC CGCGGCTCCG 2461 GGCGTATATG CTCCGCATTG GTCTTGACCA ACTCTATCAG AGCTTGGTTG ACGGCAATTT 2521 CGATGATGCA GCTTGGGCGC AGGGTCGATG CGACGCAATC GTCCGATCCG GAGCCGGGAC 2581 TGTCGGGCGT ACACAAATCG CCCGCAGAAG CGCGGCCGTC TGGACCGATG GCTGTGTAGA 2641 AGTACTCGCC GATAGTGGAA ACCGACGCCC CAGCACTCGT CCGAGGGCAA AGGAATAGCA 2701 CGTACTACGA GATTTCGATT CCACCGCCGC CTTCTATGAA AGGTTGGGCT TCGGAATCGT 2761 TTTCCGGGAC GCCGGCTGGA TGATCCTCCA GCGCGGGGAT CTCATGCTGG AGTTCTTCGC 2821 CCACCCCAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA 2881 TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA 2941 TGTATCTTAT CATGTCTGTA TACCGTCGAC CTCTAGCTAG AGCTTGGCGT AATCATGGTC 3001 ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG 3061 AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT 3121 GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG 3181 CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA 3241 CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 3301 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 3361 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 3421 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 3481 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 3541 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 3601 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 3661 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 3721 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 3781 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 3841 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 3901 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 3961 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 4021 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 4081 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 4141 GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG 4201 TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA 4261 GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC 4321 AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC 4381 TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC 4441 AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC 4501 GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC 4561 CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT 4621 GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC 4681 ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG 4741 TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG 4801 CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT 4861 CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC 4921 ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA 4981 AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA 5041 TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA 5101 AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGTC //pcDNA5/FRT/TO "Times New RomanOXC;ZCAG_enh"Times New RomanOXC,CMV_immearly_pro"Times New RomanOXCɆ CMV_fwd_pri"Times New RomanOX}CMV_pro"Times New Roman)OXCCMV2_pro"Times New Roman>OXC4[tetO"Times New RomanOXCɆe}LNCX_pri"Times New RomanOXAR BGH_rev_pri"Times New RomanOXCD' bGH_PA_term"Times New RomanOXCCrFRT"Times New RomanOXCɆ_m ORF 2"Times New RomanOXu hygroB"Times New Roman(OXCx ORF 2"Times New RomanOXC t  SV40_PA_term"Times New RomanOXCɆU h  EBV_rev_pri"Times New RomanOX M13_reverse_pri"Times New RomanOXC M13_pUC_rev_pri"Times New RomanOXC lac_pro"Times New RomanOXCɆ'  pBR322_origin"Times New RomanOX-ORF 1"Times New RomanOXC- Ampicillin"Times New Roman(OXCAmpR_pro"Times New Roman OXNruI"Times New Romank\k OXdAflII(975),HindIII(978),KpnI(988),BamHI(996),EcoRV(1031),NotI(1046),XhoI(1052),ApaI(1068),BclI(1082)"Times New Roman>G``6 OXMscI"Times New Romanw99)) OXSacII"Times New Roman Th{w{ OXFspI"Times New RomanT[ OXtest"Times New RomanI, L:Z OXtest"Times New Roman^ EXbQb