Sequence of pcDNA5_FRT_V5_His

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Sequence of pcDNA5_FRT_V5_His_TOPO

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Sequence of pcDNA5/FRT/V5 His TOPO : 5094 BP; 1182 A; 1326 C; 1307 G; 1279 T; 0 other;

.......... BglII(12) .......... .......... .......... ..........

1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 60

1 ctgcctagcc ctctagaggg ctaggggata ccacgtgaga gtcatgttag acgagactac 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120

61 ggcgtatcaa ttcggtcata gacgagggac gaacacacaa cctccagcga ctcatcacgc 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180

121 gctcgtttta aattcgatgt tgttccgttc cgaactggct gttaacgtac ttcttagacg 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240

181 aatcccaatc cgcaaaacgc gacgaagcgc tacatgcccg gtctatatgc gcaactgtaa 240

.......... .......... .......... .......... .......... ..........

SpeI(249) .......... .......... .......... .......... ..........

241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 300

241 ctaataactg atcaataatt atcattagtt aatgccccag taatcaagta tcgggtatat 300

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 360

301 acctcaaggc gcaatgtatt gaatgccatt taccgggcgg accgactggc gggttgctgg 360

.......... ....... CMV_immearly_pro CAG_enh ....... ..........

.......... .......... .......... .......... .......... ..........

361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 420

361 gggcgggtaa ctgcagttat tactgcatac aagggtatca ttgcggttat ccctgaaagg 420

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 480

421 taactgcagt tacccacctc ataaatgcca tttgacgggt gaaccgtcat gtagttcaca 480

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 540

481 tagtatacgg ttcatgcggg ggataactgc agttactgcc atttaccggg cggaccgtaa 540

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 600

541 tacgggtcat gtactggaat accctgaaag gatgaaccgt catgtagatg cataatcagt 600

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 660

601 agcgataatg gtaccactac gccaaaaccg tcatgtagtt acccgcacct atcgccaaac 660

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 720

661 tgagtgcccc taaaggttca gaggtggggt aactgcagtt accctcaaac aaaaccgtgg 720

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 780

721 ttttagttgc cctgaaaggt tttacagcat tgttgaggcg gggtaactgc gtttacccgc 780

.......... ....... CMV_immearly_pro ....... ......... CMV_fwd_pri

.......... .......... .......... .......... .......... ..........

781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 840

781 catccgcaca tgccaccctc cagatatatt cgtctcgaga gaccgattga tctcttgggt 840

CMV_fwd_pri ..... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 900

841 gacgaatgac cgaatagctt taattatgct gagtgatatc cctctgggtt cgaccgatcg 900

CMV_immearly_pro .... .......... T7_pro .......... ..........

.......... .......... .......... KpnI(939) .......... ..........

901 gtttaaactt aagctcgccc ttaagggcga gcttggtacc gagctcggat ccgaaggtaa 960

901 caaatttgaa ttcgagcggg aattcccgct cgaaccatgg ctcgagccta ggcttccatt 960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 gcctatccct aaccctctcc tcggtctcga ttctacgcgt accggtcatc atcaccatca 1020

961 cggataggga ttgggagagg agccagagct aagatgcgca tggccagtag tagtggtagt 1020

.......... V5 .......... .......... .......... 6xHis

.......... BclI(1040) .......... .......... .......... ..........

1021 ccattgagtt taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt 1080

1021 ggtaactcaa atttgggcga ctagtcggag ctgacacgga agatcaacgg tcggtagaca 1080

.......... .......... ........ BGH_rev_pri bGH_PA_term ........

.......... .......... .......... .......... .......... ..........

1081 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc 1140

1081 acaaacgggg agggggcacg gaaggaactg ggaccttcca cggtgagggt gacaggaaag 1140

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg 1200

1141 gattatttta ctcctttaac gtagcgtaac agactcatcc acagtaagat aagacccccc 1200

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga 1260

1201 accccacccc gtcctgtcgt tccccctcct aacccttctg ttatcgtccg tacgacccct 1260

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc 1320

1261 acgccacccg agataccgaa gactccgcct ttcttggtcg accccgagat cccccatagg 1320

bGH_PA_term ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac 1380

1321 ggtgcgcggg acatcgccgc gtaattcgcg ccgcccacac caccaatgcg cgtcgcactg 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc 1440

1381 gcgatgtgaa cggtcgcggg atcgcgggcg aggaaagcga aagaagggaa ggaaagagcg 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg tccctttagg gttccgattt 1500

1441 gtgcaagcgg ccgaaagggg cagttcgaga tttagccccc agggaaatcc caaggctaaa 1500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtacctag 1560

1501 tcacgaaatg ccgtggagct ggggtttttt gaactaatcc cactaccaag tgcatggatc 1560

.......... .......... .......... .......... .......... ..........

.......... .......... XbaI(1587) .......... .......... MscI(1612)

1561 aagttcctat tccgaagttc ctattctcta gaaagtatag gaacttcctt ggccaaaaag 1620

1561 ttcaaggata aggcttcaag gataagagat ctttcatatc cttgaaggaa ccggtttttc 1620

.......... .......... FRT .......... ORF 1 ..........

.......... .......... .......... .......... .......... ..........

1621 cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc 1680

1621 ggacttgagt ggcgctgcag acagctcttc aaagactagc ttttcaagct gtcgcagagg 1680

.......... .......... ORF 1 hygroB .......... ..........

.......... .......... .......... .......... .......... ..........

1681 gacctgatgc agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg 1740

1681 ctggactacg tcgagagcct cccgcttctt agagcacgaa agtcgaagct acatcctccc 1740

.......... ......... ORF 1 hygroB ORF 1 ......... ..........

.......... .......... .......... .......... .......... ..........

1741 cgtggatatg tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt 1800

1741 gcacctatac aggacgccca tttatcgacg cggctaccaa agatgtttct agcaatacaa 1800

.......... ...... ORF 1 hygroB ORF 1 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 tatcggcact ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattc 1860

1801 atagccgtga aacgtagccg gcgcgagggc taaggccttc acgaactgta accccttaag 1860

.......... ...... ORF 1 hygroB ORF 1 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 agcgagagcc tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg 1920

1861 tcgctctcgg actggataac gtagagggcg gcacgtgtcc cacagtgcaa cgttctggac 1920

.......... ...... ORF 1 hygroB ORF 1 ...... .......... ..........

.......... .......... PstI(1949) .......... .......... ..........

1921 cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct 1980

1921 ggactttggc ttgacgggcg acaagacgtc ggccagcgcc tccggtacct acgctagcga 1980

.......... ...... ORF 1 hygroB ORF 1 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 gcggccgatc ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa 2040

1981 cgccggctag aatcggtctg ctcgcccaag ccgggtaagc ctggcgttcc ttagccagtt 2040

.......... ...... ORF 1 hygroB ORF 1 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tacactacat ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa 2100

2041 atgtgatgta ccgcactaaa gtatacgcgc taacgactag gggtacacat agtgaccgtt 2100

.......... ...... ORF 1 hygroB ORF 1 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt 2160

2101 tgacactacc tgctgtggca gtcacgcagg cagcgcgtcc gagagctact cgactacgaa 2160

.......... ...... ORF 1 hygroB ORF 1 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 tgggccgagg actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat 2220

2161 acccggctcc tgacggggct tcaggccgtg gagcacgtgc gcctaaagcc gaggttgtta 2220

.......... ...... ORF 1 hygroB ORF 1 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 gtcctgacgg acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg 2280

2221 caggactgcc tgttaccggc gtattgtcgc cagtaactga cctcgctccg ctacaagccc 2280

.......... ...... ORF 1 hygroB ORF 1 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 gattcccaat acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag 2340

2281 ctaagggtta tgctccagcg gttgtagaag aagacctccg gcaccaaccg aacatacctc 2340

.......... ...... ORF 1 hygroB ORF 1 ...... .......... ..........

.......... .......... .......... .......... ......... SacII(2392)

2341 cagcagacgc gctacttcga gcggaggcat ccggagcttg caggatcgcc gcggctccgg 2400

2341 gtcgtctgcg cgatgaagct cgcctccgta ggcctcgaac gtcctagcgg cgccgaggcc 2400

......... ORF 1 hygroB ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc 2460

2401 cgcatatacg aggcgtaacc agaactggtt gagatagtct cgaaccaact gccgttaaag 2460

.......... ......... hygroB ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact 2520

2461 ctactacgtc gaacccgcgt cccagctacg ctgcgttagc aggctaggcc tcggccctga 2520

.......... ......... hygroB ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa 2580

2521 cagcccgcat gtgtttagcg ggcgtcttcg cgccggcaga cctggctacc gacacatctt 2580

.......... ......... hygroB ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 gtactcgccg atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa ggaatagcac 2640

2581 catgagcggc tatcaccttt ggctgcgggg tcgtgagcag gctcccgttt ccttatcgtg 2640

.......... ......... hygroB ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 gtactacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt 2700

2641 catgatgctc taaagctaag gtggcggcgg aagatacttt ccaacccgaa gccttagcaa 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc 2760

2701 aaggccctgc ggccgaccta ctaggaggtc gcgcccctag agtacgacct caagaagcgg 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 2820

2761 gtggggttga acaaataacg tcgaatatta ccaatgttta tttcgttatc gtagtgttta 2820

.......... .......... ......... SV40_PA_term ......... ..........

.......... .......... .......... .......... .......... ..........

2821 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa a ctcatcaat 2880

2821 aagtgtttat ttcgtaaaaa aagtgacgta agatcaacac caaacaggtt tgagtagtta 2880

.......... ......... SV40_PA_term ........ EBV_rev_pri .........

.......... .......... .......... .......... .......... ..........

2881 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca 2940

2881 catagaatag tacagacata tggcagctgg agatcgatct cgaaccgcat tagtaccagt 2940

SV40_PA_term ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga 3000

2941 atcgacaaag gacacacttt aacaataggc gagtgttaag gtgtgttgta tgctcggcct 3000

M13_reverse_pri . M13_pUC_rev_pri ....... .......... lac_pro

.......... .......... .......... .......... .......... ..........

3001 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg 3060

3001 tcgtatttca catttcggac cccacggatt actcactcga ttgagtgtaa ttaacgcaac 3060

lac_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc 3120

3061 gcgagtgacg ggcgaaaggt cagccctttg gacagcacgg tcgacgtaat tacttagccg 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac 3180

3121 gttgcgcgcc cctctccgcc aaacgcataa cccgcgagaa ggcgaaggag cgagtgactg 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 3240

3181 agcgacgcga gccagcaagc cgacgccgct cgccatagtc gagtgagttt ccgccattat 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 3300

3241 gccaataggt gtcttagtcc cctattgcgt cctttcttgt acactcgttt tccggtcgtt 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 3360

3301 ttccggtcct tggcattttt ccggcgcaac gaccgcaaaa aggtatccga ggcgggggga 3360

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

3361 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 3420

3361 ctgctcgtag tgtttttagc tgcgagttca gtctccaccg ctttgggctg tcctgatatt 3420

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3421 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 3480

3421 tctatggtcc gcaaaggggg accttcgagg gagcacgcga gaggacaagg ctgggacggc 3480

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3481 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 3540

3481 gaatggccta tggacaggcg gaaagaggga agcccttcgc accgcgaaag agtatcgagt 3540

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3541 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 3600

3541 gcgacatcca tagagtcaag ccacatccag caagcgaggt tcgacccgac acacgtgctt 3600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3601 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 3660

3601 ggggggcaag tcgggctggc gacgcggaat aggccattga tagcagaact caggttgggc 3660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 3720

3661 cattctgtgc tgaatagcgg tgaccgtcgt cggtgaccat tgtcctaatc gtctcgctcc 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg 3780

3721 atacatccgc cacgatgtct caagaacttc accaccggat tgatgccgat gtgatcttcc 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 3840

3781 tgtcataaac catagacgcg agacgacttc ggtcaatgga agcctttttc tcaaccatcg 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 3900

3841 agaactaggc cgtttgtttg gtggcgacca tcgccaccaa aaaaacaaac gttcgtcgtc 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 3960

3901 taatgcgcgt ctttttttcc tagagttctt ctaggaaact agaaaagatg ccccagactg 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 4020

3961 cgagtcacct tgcttttgag tgcaattccc taaaaccagt actctaatag tttttcctag 4020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 4080

4021 aagtggatct aggaaaattt aatttttact tcaaaattta gttagatttc atatatactc 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 4140

4081 atttgaacca gactgtcaat ggttacgaat tagtcactcc gtggatagag tcgctagaca 4140

.......... .......... ....... ORF 3 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

4141 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 4200

4141 gataaagcaa gtaggtatca acggactgag gggcagcaca tctattgatg ctatgccctc 4200

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 4260

4201 ccgaatggta gaccggggtc acgacgttac tatggcgctc tgggtgcgag tggccgaggt 4260

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 4320

4261 ctaaatagtc gttatttggt cggtcggcct tcccggctcg cgtcttcacc aggacgttga 4320

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 4380

4321 aataggcgga ggtaggtcag ataattaaca acggcccttc gatctcattc atcaagcggt 4380

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... FspI(4393) .......... .......... .......... ..........

4381 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg 4440

4381 caattatcaa acgcgttgca acaacggtaa cgatgtccgt agcaccacag tgcgagcagc 4440

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 4500

4441 aaaccatacc gaagtaagtc gaggccaagg gttgctagtt ccgctcaatg tactaggggg 4500

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 4560

4501 tacaacacgt tttttcgcca atcgaggaag ccaggaggct agcaacagtc ttcattcaac 4560

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 4620

4561 cggcgtcaca atagtgagta ccaataccgt cgtgacgtat taagagaatg acagtacggt 4620

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 4680

4621 aggcattcta cgaaaagaca ctgaccactc atgagttggt tcagtaagac tcttatcaca 4680

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc 4740

4681 tacgccgctg gctcaacgag aacgggccgc agttatgccc tattatggcg cggtgtatcg 4740

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 4800

4741 tcttgaaatt ttcacgagta gtaacctttt gcaagaagcc ccgcttttga gagttcctag 4800

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 4860

4801 aatggcgaca actctaggtc aagctacatt gggtgagcac gtgggttgac tagaagtcgt 4860

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 4920

4861 agaaaatgaa agtggtcgca aagacccact cgtttttgtc cttccgtttt acggcgtttt 4920

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 4980

4921 ttcccttatt cccgctgtgc ctttacaact tatgagtatg agaaggaaaa agttataata 4980

....... ORF 3 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 5040

4981 acttcgtaaa tagtcccaat aacagagtac tcgcctatgt ataaacttac ataaatcttt 5040

.......... .......... .......... AmpR_pro .......... ..........

.......... .......... .......... .......... .......... ..........

5041 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc...... 5094

5041 ttatttgttt atccccaagg cgcgtgtaaa ggggcttttc acggtggact gcag...... 5094

.......... .......... .......... .......... .......... ..........