Sequence of pcDNA

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Sequence of pcDNA_DEST40

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Sequence of pcDNA DEST40 : 7143 BP; 1734 A; 1766 C; 1783 G; 1860 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 60

1 ctgcctagcc ctctagaggg ctaggggata ccacgtgaga gtcatgttag acgagactac 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120

61 ggcgtatcaa ttcggtcata gacgagggac gaacacacaa cctccagcga ctcatcacgc 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180

121 gctcgtttta aattcgatgt tgttccgttc cgaactggct gttaacgtac ttcttagacg 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240

181 aatcccaatc cgcaaaacgc gacgaagcgc tacatgcccg gtctatatgc gcaactgtaa 240

.......... .......... .......... .......... .......... ..........

SpeI(249) .......... .......... .......... .......... ..........

241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 300

241 ctaataactg atcaataatt atcattagtt aatgccccag taatcaagta tcgggtatat 300

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 360

301 acctcaaggc gcaatgtatt gaatgccatt taccgggcgg accgactggc gggttgctgg 360

.......... ....... CMV_immearly_pro CAG_enh ....... ..........

.......... .......... .......... .......... .......... ..........

361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 420

361 gggcgggtaa ctgcagttat tactgcatac aagggtatca ttgcggttat ccctgaaagg 420

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 480

421 taactgcagt tacccacctc ataaatgcca tttgacgggt gaaccgtcat gtagttcaca 480

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

NdeI(484) .......... .......... .......... .......... ..........

481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 540

481 tagtatacgg ttcatgcggg ggataactgc agttactgcc atttaccggg cggaccgtaa 540

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 600

541 tacgggtcat gtactggaat accctgaaag gatgaaccgt catgtagatg cataatcagt 600

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 660

601 agcgataatg gtaccactac gccaaaaccg tcatgtagtt acccgcacct atcgccaaac 660

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 720

661 tgagtgcccc taaaggttca gaggtggggt aactgcagtt accctcaaac aaaaccgtgg 720

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 780

721 ttttagttgc cctgaaaggt tttacagcat tgttgaggcg gggtaactgc gtttacccgc 780

.......... ....... CMV_immearly_pro ....... ......... CMV_fwd_pri

.......... .......... .......... SacI(818) .......... ..........

781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 840

781 catccgcaca tgccaccctc cagatatatt cgtctcgaga gaccgattga tctcttgggt 840

CMV_fwd_pri ..... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt 900

841 gacgaatgac cgaatagctt taattatgct gagtgatatc cctctgggtt cgaccgatca 900

CMV_immearly_pro .... .......... T7_pro .......... ..........

.......... .......... .......... .......... .......... ..........

901 taagctatca acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg atataaatat 960

901 attcgatagt tgttcaaaca tgttttttcg acttgctctt tgcattttac tatatttata 960

.......... .......... .......... attR1 attR2 ..........

.......... .......... .......... .......... .......... ..........

961 caatatatta aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca 1020

961 gttatataat ttaatctaaa acgtattttt tgtctgatgt attatgacat tttgtgttgt 1020

.......... ......... attR1 attR2 ......... .......... ..........

.......... NotI(1037) .......... .......... .......... ..........

1021 tatccagtca ctatggcggc cgcattaggc accccaggct ttacacttta tgcttccggc 1080

1021 ataggtcagt gataccgccg gcgtaatccg tggggtccga aatgtgaaat acgaaggccg 1080

attR1 attR2 ........ .......... .......... lac_pro ..........

.......... .......... .......... .......... .......... ..........

1081 tcgtataatg tgtggatttt gagttaggat ccggcgagat tttcaggagc taaggaagct 1140

1081 agcatattac acacctaaaa ctcaatccta ggccgctcta aaagtcctcg attccttcga 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa 1200

1141 ttttacctct ttttttagtg acctatatgg tggcaactat atagggttac cgtagcattt 1200

.......... .......... ........ ORF 1 CAT/CamR ........ ..........

.......... .......... .......... .......... .......... ..........

1201 gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg 1260

1201 cttgtaaaac tccgtaaagt cagtcaacga gttacatgga tattggtctg gcaagtcgac 1260

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1261 gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt 1320

1261 ctataatgcc ggaaaaattt ctggcatttc tttttattcg tgttcaaaat aggccggaaa 1320

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... ......... EcoRI(1357) ......... ..........

1321 attcacattc ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac 1380

1321 taagtgtaag aacgggcgga ctacttacga gtaggcctta aggcataccg ttactttctg 1380

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1381 ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact 1440

1381 ccactcgacc actataccct atcacaagtg ggaacaatgt ggcaaaaggt actcgtttga 1440

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1441 gaaacgtttt catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata 1500

1441 ctttgcaaaa gtagcgagac ctcacttatg gtgctgctaa aggccgtcaa agatgtgtat 1500

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1501 tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt 1560

1501 ataagcgttc tacaccgcac aatgccactt ttggaccgga taaagggatt tcccaaataa 1560

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1561 gagaatatgt ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac 1620

1561 ctcttataca aaaagcagag tcggttaggg acccactcaa agtggtcaaa actaaatttg 1620

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gtggccaata tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa 1680

1621 caccggttat acctgttgaa gaagcggggg caaaagtggt acccgtttat aatatgcgtt 1680

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1681 ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtctg tgatggcttc 1740

1681 ccgctgttcc acgactacgg cgaccgctaa gtccaagtag tacggcagac actaccgaag 1740

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1741 catgtcggca gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg 1800

1741 gtacagccgt cttacgaatt acttaatgtt gtcatgacgc tactcaccgt cccgccccgc 1800

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1801 taaagatctg gatccggctt actaaaagcc agataacagt atgcgtattt gcgcgctgat 1860

1801 atttctagac ctaggccgaa tgattttcgg tctattgtca tacgcataaa cgcgcgacta 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 ttttgcggta taagaatata tactgatatg tatacccgaa gtatgtcaaa aagaggtgtg 1920

1861 aaaacgccat attcttatat atgactatac atatgggctt catacagttt ttctccacac 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ctatgaagca gcgtattaca gtgacagttg acagcgacag ctatcagttg ctcaaggcat 1980

1921 gatacttcgt cgcataatgt cactgtcaac tgtcgctgtc gatagtcaac gagttccgta 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 atatgatgtc aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct 2040

1981 tatactacag ttatagaggc cagaccattc gtgttggtac gtcttacttc gggcagcaga 2040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 gcgtgccgaa cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat 2100

2041 cgcacggctt gcgacctttc gccttttagt ccttccctac cgactccagc gggccaaata 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 tgaaatgaac ggctcttttg ctgacgagaa cagggactgg tgaaatgcag tttaaggttt 2160

2101 actttacttg ccgagaaaac gactgctctt gtccctgacc actttacgtc aaattccaaa 2160

.......... .......... .......... .......... .......... ccdB

.......... .......... .......... .......... .......... ..........

2161 acacctataa aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg 2220

2161 tgtggatatt ttctctctcg gcaatagcag acaaacacct acatgtctca ctataataac 2220

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 acacgcccgg gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag 2280

2221 tgtgcgggcc cgctgcctac cactaggggg accggtcacg tgcagacgac agtctatttc 2280

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 tctcccgtga actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca 2340

2281 agagggcact tgaaatgggc caccacgtat agcccctact ttcgaccgcg tactactggt 2340

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 ccgatatggc cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc 2400

2341 ggctataccg gtcacacggc cagaggcaat agccccttct tcaccgacta gagtcggtgg 2400

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 gcgaaaatga catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct 2460

2401 cgcttttact gtagtttttg cggtaattgg actacaagac cccttatatt tacagtccga 2460

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ccgttataca cagccagtct gcaggtcgac catagtgact ggatatgttg tgttttacag 2520

2461 ggcaatatgt gtcggtcaga cgtccagctg gtatcactga cctatacaac acaaaatgtc 2520

.......... .......... .......... ......... attR2 attR1 .........

.......... .......... .......... .......... .......... ..........

2521 tattatgtag tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt 2580

2521 ataatacatc agacaaaaaa tacgttttag attaaattat ataactataa atatagtaaa 2580

.......... ......... attR2 attR1 ......... .......... ..........

.......... .......... .......... XbaI(2619) .......... ..........

2581 tacgtttctc gttcagcttt cttgtacaaa gtggttgatc tagagggccc gcggttcgaa 2640

2581 atgcaaagag caagtcgaaa gaacatgttt caccaactag atctcccggg cgccaagctt 2640

attR2 attR1 ........ .......... .......... .......... ..........

.......... .......... .......... .......... AgeI(2686) ..........

2641 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgcgtaccgg tcatcatcac 2700

2641 ccattcggat agggattggg agaggagcca gagctaagat gcgcatggcc agtagtagtg 2700

.......... .......... V5 .......... .......... 6xHis

.......... .......... .......... .......... .......... ..........

2701 catcaccatt gagtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca 2760

2701 gtagtggtaa ctcaaatttg ggcgactagt cggagctgac acggaagatc aacggtcggt 2760

6xHis .......... ........ BGH_rev_pri bGH_PA_term ........

.......... .......... .......... .......... .......... ..........

2761 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc 2820

2761 agacaacaaa cggggagggg gcacggaagg aactgggacc ttccacggtg agggtgacag 2820

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg 2880

2821 gaaaggatta ttttactcct ttaacgtagc gtaacagact catccacagt aagataagac 2880

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct 2940

2881 cccccacccc accccgtcct gtcgttcccc ctcctaaccc ttctgttatc gtccgtacga 2940

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg 3000

2941 cccctacgcc acccgagata ccgaagactc cgcctttctt ggtcgacccc gagatccccc 3000

......... bGH_PA_term ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc 3060

3001 ataggggtgc gcgggacatc gccgcgtaat tcgcgccgcc cacaccacca atgcgcgtcg 3060

.......... .......... .......... .......... f1_origin ..........

.......... .......... .......... .......... .......... ..........

3061 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt 3120

3061 cactggcgat gtgaacggtc gcgggatcgc gggcgaggaa agcgaaagaa gggaaggaaa 3120

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc 3180

3121 gagcggtgca agcggccgaa aggggcagtt cgagatttag cccccgaggg aaatcccaag 3180

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt 3240

3181 gctaaatcac gaaatgccgt ggagctgggg ttttttgaac taatcccact accaagtgca 3240

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt 3300

3241 tcacccggta gcgggactat ctgccaaaaa gcgggaaact gcaacctcag gtgcaagaaa 3300

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt 3360

3301 ttatcacctg agaacaaggt ttgaccttgt tgtgagttgg gatagagcca gataagaaaa 3360

.......... f1_origin .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa 3420

3361 ctaaatattc cctaaaacgg ctaaagccgg ataaccaatt ttttactcga ctaaattgtt 3420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag 3480

3421 tttaaattgc gcttaattaa gacaccttac acacagtcaa tcccacacct ttcaggggtc 3480

.......... .......... ......... pBABE_3_pri SV40_enh SV40_pro

.......... .......... .......... .......... .......... ..........

3481 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg 3540

3481 cgaggggtcg tccgtcttca tacgtttcgt acgtagagtt aatcagtcgt tggtccacac 3540

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag 3600

3541 ctttcagggg tccgaggggt cgtccgtctt catacgtttc gtacgtagag ttaatcagtc 3600

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc 3660

3601 gttggtatca gggcggggat tgaggcgggt agggcgggga ttgaggcggg tcaaggcggg 3660

.......... ...... SV40_enh SV40_pro ..... SV40_origin .........

.......... .......... .......... .......... .......... ..........

3661 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg 3720

3661 taagaggcgg ggtaccgact gattaaaaaa aataaatacg tctccggctc cggcggagac 3720

.......... ..... SV40_pro SV40_origin ... SV40pro_F_pri ........

.......... .......... .......... .......... StuI(3763) ..........

3721 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa 3780

3721 ggagactcga taaggtcttc atcactcctc cgaaaaaacc tccggatccg aaaacgtttt 3780

SV40_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 agctcccggg agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt 3840

3781 tcgagggccc tcgaacatat aggtaaaagc ctagactagt tctctgtcct actcctagca 3840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc 3900

3841 aagcgtacta acttgttcta cctaacgtgc gtccaagagg ccggcgaacc cacctctccg 3900

.......... .......... ........ ORF 3 Neomycin ........ ..........

.......... .......... .......... .......... .......... ..........

3901 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc 3960

3901 ataagccgat actgacccgt gttgtctgtt agccgacgag actacggcgg cacaaggccg 3960

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... NarI(3974) .......... .......... .......... ..........

3961 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg 4020

3961 acagtcgcgt ccccgcgggc caagaaaaac agttctggct ggacaggcca cgggacttac 4020

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag 4080

4021 ttgacgtcct gctccgtcgc gccgatagca ccgaccggtg ctgcccgcaa ggaacgcgtc 4080

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg 4140

4081 gacacgagct gcaacagtga cttcgccctt ccctgaccga cgataacccg cttcacggcc 4140

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg 4200

4141 ccgtcctaga ggacagtaga gtggaacgag gacggctctt tcataggtag taccgactac 4200

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4201 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac 4260

4201 gttacgccgc cgacgtatgc gaactaggcc gatggacggg taagctggtg gttcgctttg 4260

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4261 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg 4320

4261 tagcgtagct cgctcgtgca tgagcctacc ttcggccaga acagctagtc ctactagacc 4320

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4321 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc 4380

4321 tgcttctcgt agtccccgag cgcggtcggc ttgacaagcg gtccgagttc cgcgcgtacg 4380

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4381 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg 4440

4381 ggctgccgct cctagagcag cactgggtac cgctacggac gaacggctta tagtaccacc 4440

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4441 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc 4500

4441 ttttaccggc gaaaagacct aagtagctga caccggccga cccacaccgc ctggcgatag 4500

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4501 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc 4560

4501 tcctgtatcg caaccgatgg gcactataac gacttctcga accgccgctt acccgactgg 4560

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4561 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc 4620

4561 cgaaggagca cgaaatgcca tagcggcgag ggctaagcgt cgcgtagcgg aagatagcgg 4620

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4621 ttcttgacga gttcttctga gcgggactct ggggttcgcg aaatgaccga ccaagcgacg 4680

4621 aagaactgct caagaagact cgccctgaga ccccaagcgc tttactggct ggttcgctgc 4680

ORF 3 Neomycin ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc 4740

4681 gggttggacg gtagtgctct aaagctaagg tggcggcgga agatactttc caacccgaag 4740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag 4800

4741 ccttagcaaa aggccctgcg gccgacctac taggaggtcg cgcccctaga gtacgacctc 4800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc 4860

4801 aagaagcggg tggggttgaa caaataacgt cgaatattac caatgtttat ttcgttatcg 4860

.......... .......... ......... SV40_PA_term ......... ..........

.......... .......... .......... .......... .......... ..........

4861 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 4920

4861 tagtgtttaa agtgtttatt tcgtaaaaaa agtgacgtaa gatcaacacc aaacaggttt 4920

.......... ......... SV40_PA_term ......... ......... EBV_rev_pri

.......... .......... .......... .......... .......... ..........

4921 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa 4980

4921 gagtagttac atagaatagt acagacatat ggcagctgga gatcgatctc gaaccgcatt 4980

SV40_PA_term ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata 5040

4981 agtaccagta tcgacaaagg acacacttta acaataggcg agtgttaagg tgtgttgtat 5040

.... M13_reverse_pri M13_pUC_rev_pri .... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta 5100

5041 gctcggcctt cgtatttcac atttcggacc ccacggatta ctcactcgat tgagtgtaat 5100

.......... lac_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa 5160

5101 taacgcaacg cgagtgacgg gcgaaaggtc agccctttgg acagcacggt cgacgtaatt 5160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg 5220

5161 acttagccgg ttgcgcgccc ctctccgcca aacgcataac ccgcgagaag gcgaaggagc 5220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag 5280

5221 gagtgactga gcgacgcgag ccagcaagcc gacgccgctc gccatagtcg agtgagtttc 5280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa 5340

5281 cgccattatg ccaataggtg tcttagtccc ctattgcgtc ctttcttgta cactcgtttt 5340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc 5400

5341 ccggtcgttt tccggtcctt ggcatttttc cggcgcaacg accgcaaaaa ggtatccgag 5400

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

5401 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca 5460

5401 gcggggggac tgctcgtagt gtttttagct gcgagttcag tctccaccgc tttgggctgt 5460

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5461 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg 5520

5461 cctgatattt ctatggtccg caaaggggga ccttcgaggg agcacgcgag aggacaaggc 5520

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5521 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct 5580

5521 tgggacggcg aatggcctat ggacaggcgg aaagagggaa gcccttcgca ccgcgaaaga 5580

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5581 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt 5640

5581 gtatcgagtg cgacatccat agagtcaagc cacatccagc aagcgaggtt cgacccgaca 5640

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5641 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag 5700

5641 cacgtgcttg gggggcaagt cgggctggcg acgcggaata ggccattgat agcagaactc 5700

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5701 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc 5760

5701 aggttgggcc attctgtgct gaatagcggt gaccgtcgtc ggtgaccatt gtcctaatcg 5760

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5761 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac 5820

5761 tctcgctcca tacatccgcc acgatgtctc aagaacttca ccaccggatt gatgccgatg 5820

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5821 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga 5880

5821 tgatcttctt gtcataaacc atagacgcga gacgacttcg gtcaatggaa gcctttttct 5880

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5881 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc 5940

5881 caaccatcga gaactaggcc gtttgtttgg tggcgaccat cgccaccaaa aaaacaaacg 5940

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5941 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 6000

5941 ttcgtcgtct aatgcgcgtc tttttttcct agagttcttc taggaaacta gaaaagatgc 6000

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6001 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca 6060

6001 cccagactgc gagtcacctt gcttttgagt gcaattccct aaaaccagta ctctaatagt 6060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt 6120

6061 ttttcctaga agtggatcta ggaaaattta atttttactt caaaatttag ttagatttca 6120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca 6180

6121 tatatactca tttgaaccag actgtcaatg gttacgaatt agtcactccg tggatagagt 6180

.......... .......... .......... ....... ORF 3 Ampicillin .......

.......... .......... .......... .......... .......... ..........

6181 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg 6240

6181 cgctagacag ataaagcaag taggtatcaa cggactgagg ggcagcacat ctattgatgc 6240

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca 6300

6241 tatgccctcc cgaatggtag accggggtca cgacgttact atggcgctct gggtgcgagt 6300

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt 6360

6301 ggccgaggtc taaatagtcg ttatttggtc ggtcggcctt cccggctcgc gtcttcacca 6360

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt 6420

6361 ggacgttgaa ataggcggag gtaggtcaga taattaacaa cggcccttcg atctcattca 6420

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca 6480

6421 tcaagcggtc aattatcaaa cgcgttgcaa caacggtaac gatgtccgta gcaccacagt 6480

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6481 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca 6540

6481 gcgagcagca aaccataccg aagtaagtcg aggccaaggg ttgctagttc cgctcaatgt 6540

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6541 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga 6600

6541 actagggggt acaacacgtt ttttcgccaa tcgaggaagc caggaggcta gcaacagtct 6600

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6601 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact 6660

6601 tcattcaacc ggcgtcacaa tagtgagtac caataccgtc gtgacgtatt aagagaatga 6660

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6661 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga 6720

6661 cagtacggta ggcattctac gaaaagacac tgaccactca tgagttggtt cagtaagact 6720

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6721 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg 6780

6721 cttatcacat acgccgctgg ctcaacgaga acgggccgca gttatgccct attatggcgc 6780

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6781 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc 6840

6781 ggtgtatcgt cttgaaattt tcacgagtag taaccttttg caagaagccc cgcttttgag 6840

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6841 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga 6900

6841 agttcctaga atggcgacaa ctctaggtca agctacattg ggtgagcacg tgggttgact 6900

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6901 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat 6960

6901 agaagtcgta gaaaatgaaa gtggtcgcaa agacccactc gtttttgtcc ttccgtttta 6960

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6961 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt 7020

6961 cggcgttttt tcccttattc ccgctgtgcc tttacaactt atgagtatga gaaggaaaaa 7020

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7021 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt 7080

7021 gttataataa cttcgtaaat agtcccaata acagagtact cgcctatgta taaacttaca 7080

.......... .......... .......... .......... AmpR_pro ..........

.......... .......... .......... .......... .......... ..........

7081 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac 7140

7081 taaatctttt tatttgttta tccccaaggc gcgtgtaaag gggcttttca cggtggactg 7140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7141 gtc....... .......... .......... .......... .......... .......... 7143

7141 cag....... .......... .......... .......... .......... .......... 7143

.......... .......... .......... .......... .......... ..........