jjORF 2"Times New RomanjC  SV40_PA_term"Times New Romanj, EBV_rev_pri"Times New RomanjC  pBR322_origin"Times New RomanjCɆ! }ORF 3"Times New Romanj! } Ampicillin"Times New RomanjCAmpR_pro"Times New RomanjCD f1_origin"Times New RomanjCɆK{ synth_PA_term"Times New RomanjKTB"Times New RomanjCK up_PA_term"Times New Roman*jC RVpri3_pri"Times New Roman j,KpnI(5),SacI(11),NheI(21),XhoI(32),BglII(36)"Times New Romanv;v jMscI"Times New Roman21 ( jNarI(325),SacII(334)"Times New RomanE48<-]K j PvuII(566),EcoRI(684),BstBI(688)"Times New Roman6pdrr jHindIII"Times New Roman  jEcoRV"Times New Roman# jNruI(1491),AatII(1527)"Times New Roman jXbaI"Times New Roman !   j!HpaI(1927),BamHI(2029),SalI(2035)"Times New Roman a;3`VwVw jAseI"Times New Roman  jNotI"Times New RomanD!! jtest"Times New RomanIL