Sequence of phRL-CMV

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Sequence of phRL-CMV

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Sequence of phRL-CMV : 4079 BP; 1060 A; 968 C; 1011 G; 1040 T; 0 other;

BglII(1) .......... .......... .......... .......... ..........

1 agatcttcaa tattggccat tagccatatt attcattggt tatatagcat aaatcaatat 60

1 tctagaagtt ataaccggta atcggtataa taagtaacca atatatcgta tttagttata 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt atattggctc 120

61 accgataacc ggtaacgtat gcaacataga tatagtatta tacatgtaaa tataaccgag 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... SpeI(158) .......... ..........

121 atgtccaata tgaccgccat gttggcattg attattgact agttattaat agtaatcaat 180

121 tacaggttat actggcggta caaccgtaac taataactga tcaataatta tcattagtta 180

.......... .......... .......... .......... ....... CMV_immearly_pro

.......... .......... .......... .......... .......... ..........

181 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa 240

181 atgccccagt aatcaagtat cgggtatata cctcaaggcg caatgtattg aatgccattt 240

.......... ....... CMV_immearly_pro ....... .......... CAG_enh

.......... .......... .......... .......... .......... ..........

241 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt 300

241 accgggcgga ccgactggcg ggttgctggg ggcgggtaac tgcagttatt actgcataca 300

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

301 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta 360

301 agggtatcat tgcggttatc cctgaaaggt aactgcagtt acccacctca taaatgccat 360

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... NdeI(393) .......... ..........

361 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc ctattgacgt 420

361 ttgacgggtg aaccgtcatg tagttcacat agtatacggt tcaggcgggg gataactgca 420

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

421 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac gggactttcc 480

421 gttactgcca tttaccgggc ggaccgtaat acgggtcatg tactggaatg ccctgaaagg 480

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

481 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca 540

481 atgaaccgtc atgtagatgc ataatcagta gcgataatgg taccactacg ccaaaaccgt 540

....... CAG_enh CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

541 gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat 600

541 catgtggtta cccgcaccta tcgccaaact gagtgcccct aaaggttcag aggtggggta 600

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa 660

601 actgcagtta ccctcaaaca aaaccgtggt tttagttgcc ctgaaaggtt ttacagcatt 660

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

661 taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag 720

661 attggggcgg ggcaactgcg tttacccgcc atccgcacat gccaccctcc agatatattc 720

.......... . CMV_immearly_pro CMV_fwd_pri CMV_pro . ..........

SacI(727) .......... .......... .......... .......... ..........

721 cagagctcgt ttagtgaacc gtcagatcac tagaagcttt attgcggtag tttatcacag 780

721 gtctcgagca aatcacttgg cagtctagtg atcttcgaaa taacgccatc aaatagtgtc 780

CMV_pro pCEP_fwd_pri LNCX_pri .......... .......... ..........

.......... .......... .......... .......... .......... PstI(836)

781 ttaaattgct aacgcagtca gtgcttctga cacaacagtc tcgaacttaa gctgcagaag 840

781 aatttaacga ttgcgtcagt cacgaagact gtgttgtcag agcttgaatt cgacgtcttc 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ttggtcgtga ggcactgggc aggtaagtat caaggttaca agacaggttt aaggagacca 900

841 aaccagcact ccgtgacccg tccattcata gttccaatgt tctgtccaaa ttcctctggt 900

.......... .......... .......... .......... chim_int ..........

.......... .......... .......... .......... .......... ..........

901 atagaaactg ggcttgtcga gacagagaag actcttgcgt ttctgatagg cacctattgg 960

901 tatctttgac ccgaacagct ctgtctcttc tgagaacgca aagactatcc gtggataacc 960

.......... .......... chim_int .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 tcttactgac atccactttg cctttctctc cacaggtgtc cactcccagt tcaattacag 1020

961 agaatgactg taggtgaaac ggaaagagag gtgtccacag gtgagggtca agttaatgtc 1020

.......... chim_int .......... .......... .......... ..........

.......... .......... .......... NheI(1058) .......... ..........

1021 ctcttaaggc tagagtactt aatacgactc actataggct agccaccatg gcttccaagg 1080

1021 gagaattccg atctcatgaa ttatgctgag tgatatccga tcggtggtac cgaaggttcc 1080

.......... .......... T7_pro .......... .......... ORF 3

.......... .......... .......... .......... .......... ..........

1081 tgtacgaccc cgagcaacgc aaacgcatga tcactgggcc tcagtggtgg gctcgctgca 1140

1081 acatgctggg gctcgttgcg tttgcgtact agtgacccgg agtcaccacc cgagcgacgt 1140

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 agcaaatgaa cgtgctggac tccttcatca actactatga ttccgagaag cacgccgaga 1200

1141 tcgtttactt gcacgacctg aggaagtagt tgatgatact aaggctcttc gtgcggctct 1200

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 acgccgtgat ttttctgcat ggtaacgctg cctccagcta cctgtggagg cacgtcgtgc 1260

1201 tgcggcacta aaaagacgta ccattgcgac ggaggtcgat ggacacctcc gtgcagcacg 1260

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 ctcacatcga gcccgtggct agatgcatca tccctgatct gatcggaatg ggtaagtccg 1320

1261 gagtgtagct cgggcaccga tctacgtagt agggactaga ctagccttac ccattcaggc 1320

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 gcaagagcgg gaatggctca tatcgcctcc tggatcacta caagtacctc accgcttggt 1380

1321 cgttctcgcc cttaccgagt atagcggagg acctagtgat gttcatggag tggcgaacca 1380

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 tcgagctgct gaaccttcca aagaaaatca tctttgtggg ccacgactgg ggggcttgtc 1440

1381 agctcgacga cttggaaggt ttcttttagt agaaacaccc ggtgctgacc ccccgaacag 1440

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 tggcctttca ctactcctac gagcaccaag acaagatcaa ggccatcgtc catgctgaga 1500

1441 accggaaagt gatgaggatg ctcgtggttc tgttctagtt ccggtagcag gtacgactct 1500

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... ......... EcoRV(1553)

1501 gtgtcgtgga cgtgatcgag tcctgggacg agtggcctga catcgaggag gatatcgccc 1560

1501 cacagcacct gcactagctc aggaccctgc tcaccggact gtagctcctc ctatagcggg 1560

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 tgatcaagag cgaagagggc gagaaaatgg tgcttgagaa taacttcttc gtcgagacca 1620

1561 actagttctc gcttctcccg ctcttttacc acgaactctt attgaagaag cagctctggt 1620

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 tgctcccaag caagatcatg cggaaactgg agcctgagga gttcgctgcc tacctggagc 1680

1621 acgagggttc gttctagtac gcctttgacc tcggactcct caagcgacgg atggacctcg 1680

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... NruI(1729) ..........

1681 cattcaagga gaagggcgag gttagacggc ctaccctctc ctggcctcgc gagatccctc 1740

1681 gtaagttcct cttcccgctc caatctgccg gatgggagag gaccggagcg ctctagggag 1740

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 tcgttaaggg aggcaagccc gacgtcgtcc agattgtccg caactacaac gcctaccttc 1800

1741 agcaattccc tccgttcggg ctgcagcagg tctaacaggc gttgatgttg cggatggaag 1800

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 gggccagcga cgatctgcct aagatgttca tcgagtccga ccctgggttc ttttccaacg 1860

1801 cccggtcgct gctagacgga ttctacaagt agctcaggct gggacccaag aaaaggttgc 1860

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 ctattgtcga gggagctaag aagttcccta acaccgagtt cgtgaaggtg aagggcctcc 1920

1861 gataacagct ccctcgattc ttcaagggat tgtggctcaa gcacttccac ttcccggagg 1920

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 acttcagcca ggaggacgct ccagatgaaa tgggtaagta catcaagagc ttcgtggagc 1980

1921 tgaagtcggt cctcctgcga ggtctacttt acccattcat gtagttctcg aagcacctcg 1980

.......... .......... ORF 3 .......... .......... ..........

.......... .... XbaI(2005) NotI(2012),EagI(2012) .... ..........

1981 gcgtgctgaa gaacgagcag taattctaga gcggccgctt cgagcagaca tgataagata 2040

1981 cgcacgactt cttgctcgtc attaagatct cgccggcgaa gctcgtctgt actattctat 2040

.......... ORF 3 .......... .......... ......... SV40_PA_term

.......... .......... .......... .......... .......... ..........

2041 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga 2100

2041 gtaactactc aaacctgttt ggtgttgatc ttacgtcact ttttttacga aataaacact 2100

........ EBV_rev_pri SV40_PA_term ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2101 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa 2160

2101 ttaaacacta cgataacgaa ataaacattg gtaatattcg acgttatttg ttcaattgtt 2160

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag 2220

2161 gttgttaacg taagtaaaat acaaagtcca agtccccctc cacaccctcc aaaaaatttc 2220

.......... .......... .......... .......... .......... ..........

.......... ......... ClaI(2250) BamHI(2257) ......... ..........

2221 caagtaaaac ctctacaaat gtggtaaaat cgataaggat ccaggtggca cttttcgggg 2280

2221 gttcattttg gagatgttta caccatttta gctattccta ggtccaccgt gaaaagcccc 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct 2340

2281 tttacacgcg ccttggggat aaacaaataa aaagatttat gtaagtttat acataggcga 2340

.......... .......... .......... .......... .......... AmpR_pro

.......... .......... .......... .......... .......... ..........

2341 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat 2400

2341 gtactctgtt attgggacta tttacgaagt tattataact ttttccttct catactcata 2400

AmpR_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc 2460

2401 agttgtaaag gcacagcggg aataagggaa aaaacgccgt aaaacggaag gacaaaaacg 2460

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg 2520

2461 agtgggtctt tgcgaccact ttcattttct acgacttcta gtcaacccac gtgctcaccc 2520

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg 2580

2521 aatgtagctt gacctagagt tgtcgccatt ctaggaactc tcaaaagcgg ggcttcttgc 2580

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga 2640

2581 aaaaggttac tactcgtgaa aatttcaaga cgatacaccg cgccataata gggcataact 2640

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta 2700

2641 gcggcccgtt ctcgttgagc cagcggcgta tgtgataaga gtcttactga accaactcat 2700

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc 2760

2701 gagtggtcag tgtcttttcg tagaatgcct accgtactgt cattctctta atacgtcacg 2760

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc 2820

2761 acggtattgg tactcactat tgtgacgccg gttgaatgaa gactgttgct agcctcctgg 2820

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg 2880

2821 cttcctcgat tggcgaaaaa acgtgttgta ccccctagta cattgagcgg aactagcaac 2880

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc 2940

2881 ccttggcctc gacttacttc ggtatggttt gctgctcgca ctgtggtgct acggacatcg 2940

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... FspI(2957) .......... .......... .......... ..........

2941 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca 3000

2941 ttaccgttgt tgcaacgcgt ttgataattg accgcttgat gaatgagatc gaagggccgt 3000

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct 3060

3001 tgttaattat ctgacctacc tccgcctatt tcaacgtcct ggtgaagacg cgagccggga 3060

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat 3120

3061 aggccgaccg accaaataac gactatttag acctcggcca ctcgcaccca gagcgccata 3120

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg 3180

3121 gtaacgtcgt gaccccggtc taccattcgg gagggcatag catcaataga tgtgctgccc 3180

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat 3240

3181 ctcagtccgt tgatacctac ttgctttatc tgtctagcga ctctatccac ggagtgacta 3240

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact 3300

3241 attcgtaacc attgacagtc tggttcaaat gagtatatat gaaatctaac taaattttga 3300

ORF 2 Ampicillin .... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat 3360

3301 agtaaaaatt aaattttcct agatccactt ctaggaaaaa ctattagagt actggtttta 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc 3420

3361 gggaattgca ctcaaaagca aggtgactcg cagtctgggg catcttttct agtttcctag 3420

.......... .......... .......... .......... ........ pBR322_origin

.......... .......... .......... .......... .......... ..........

3421 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct 3480

3421 aagaactcta ggaaaaaaag acgcgcatta gacgacgaac gtttgttttt ttggtggcga 3480

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3481 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg 3540

3481 tggtcgccac caaacaaacg gcctagttct cgatggttga gaaaaaggct tccattgacc 3540

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3541 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca 3600

3541 gaagtcgtct cgcgtctatg gtttatgaca agaagatcac atcggcatca atccggtggt 3600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3601 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc 3660

3601 gaagttcttg agacatcgtg gcggatgtat ggagcgagac gattaggaca atggtcaccg 3660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga 3720

3661 acgacggtca ccgctattca gcacagaatg gcccaacctg agttctgcta tcaatggcct 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac 3780

3721 attccgcgtc gccagcccga cttgcccccc aagcacgtgt gtcgggtcga acctcgcttg 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga 3840

3781 ctggatgtgg cttgactcta tggatgtcgc actcgatact ctttcgcggt gcgaagggct 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag 3900

3841 tccctctttc cgcctgtcca taggccattc gccgtcccag ccttgtcctc tcgcgtgctc 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg 3960

3901 cctcgaaggt ccccctttgc ggaccataga aatatcagga cagcccaaag cggtggagac 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag 4020

3961 tgaactcgca gctaaaaaca ctacgagcag tccccccgcc tcggatacct ttttgcggtc 4020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tggctcgac. 4079

4021 gttgcgccgg aaaaatgcca aggaccggaa aacgaccgga aaacgagtgt accgagctg. 4079

.......... .......... .......... .......... .......... ..........