Sequence of phRL-TK(Int-)

This sequnce graph is created using BVTech Plasmid, which is DNA sequence analysis and plasmid drawing software for Windows PCs.You can use it to plan your DNA cloning, draw high quality plasmid maps, analyse your DNA sequencing data, align sequences, and much more.

Free to try, $39.98 to buy

Payments will be processed by PayPal. PayPal is the safer, easier way to pay.

Sequence of phRL-TK(Int-)

Download the sequence and plasmid map, and then use BVTech Plasmid to view and edit the file.

If you do not have BVTech Plasmid, download the latest version of BVTech Plasmid here.

View the plasmid map on line

Sequence of phRL-TK(Int-) : 3785 BP; 910 A; 950 C; 1044 G; 881 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 agatctaaat gagtcttcgg acctcgcggg ggccgcttaa gcggtggtta gggtttgtct 60

1 tctagattta ctcagaagcc tggagcgccc ccggcgaatt cgccaccaat cccaaacaga 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 gacgcggggg gagggggaag gaacgaaaca ctctcattcg gaggcggctc ggggtttggt 120

61 ctgcgccccc ctcccccttc cttgctttgt gagagtaagc ctccgccgag ccccaaacca 120

.......... .......... .......... .......... .......... ..........

MscI(128) .......... .......... .......... .......... ..........

121 cttggtggcc acgggcacgc agaagagcgc cgcgatcctc ttaagcaccc ccccgccctc 180

121 gaaccaccgg tgcccgtgcg tcttctcgcg gcgctaggag aattcgtggg ggggcgggag 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 cgtggaggcg ggggtttggt cggcgggtgg taactggcgg gccgctgact cgggcgggtc 240

181 gcacctccgc ccccaaacca gccgcccacc attgaccgcc cggcgactga gcccgcccag 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... ..... SmaI(284) NarI(290) SacII(299)

241 gcgcgcccca gagtgtgacc ttttcggtct gctcgcagac ccccgggcgg cgccgccgcg 300

241 cgcgcggggt ctcacactgg aaaagccaga cgagcgtctg ggggcccgcc gcggcggcgc 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 gcggcgacgg gctcgctggg tcctaggctc catggggacc gtatacgtgg acaggctctg 360

301 cgccgctgcc cgagcgaccc aggatccgag gtacccctgg catatgcacc tgtccgagac 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 gagcatccgc acgactgcgg tgatattacc ggagaccttc tgcgggacga gccgggtcac 420

361 ctcgtaggcg tgctgacgcc actataatgg cctctggaag acgccctgct cggcccagtg 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 gcggctgacg cggagcgtcc gttgggcgac aaacaccagg acggggcaca ggtacactat 480

421 cgccgactgc gcctcgcagg caacccgctg tttgtggtcc tgccccgtgt ccatgtgata 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 cttgtcaccc ggaggcgcga gggactgcag gagcttcagg gagtggcgca gctgcttcat 540

481 gaacagtggg cctccgcgct ccctgacgtc ctcgaagtcc ctcaccgcgt cgacgaagta 540

.......... .......... .......... .......... .......... TATA_pro

.......... .......... .......... .......... .......... ..........

541 ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg gaagaaatat atttgcatgt 600

541 ggggcaccgg gcaacgagcg caaacgaccg ccacaggggc cttctttata taaacgtaca 600

.......... .......... TATA_pro TK_pro .......... ..........

.......... .......... .......... .......... .... EcoRI(649),BstBI(653)

601 ctttagttct atgatgacac aaaccccgcc cagcgtcttg tcattggcga attcgaacac 660

601 gaaatcaaga tactactgtg tttggggcgg gtcgcagaac agtaaccgct taagcttgtg 660

.......... ....... TATA_pro TK_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

661 gcagatgcag tcggggcggc gcggtcccag gtccacttcg catattaagg tgacgcgtgt 720

661 cgtctacgtc agccccgccg cgccagggtc caggtgaagc gtataattcc actgcgcaca 720

TATA_pro .......... TK_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 ggcctcgaac accgagcgac cctgcagcga cccgcttaaa agctctagcc accatggctt 780

721 ccggagcttg tggctcgctg ggacgtcgct gggcgaattt tcgagatcgg tggtaccgaa 780

.......... TK_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 ccaaggtgta cgaccccgag caacgcaaac gcatgatcac tgggcctcag tggtgggctc 840

781 ggttccacat gctggggctc gttgcgtttg cgtactagtg acccggagtc accacccgag 840

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 gctgcaagca aatgaacgtg ctggactcct tcatcaacta ctatgattcc gagaagcacg 900

841 cgacgttcgt ttacttgcac gacctgagga agtagttgat gatactaagg ctcttcgtgc 900

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 ccgagaacgc cgtgattttt ctgcatggta acgctgcctc cagctacctg tggaggcacg 960

901 ggctcttgcg gcactaaaaa gacgtaccat tgcgacggag gtcgatggac acctccgtgc 960

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 tcgtgcctca catcgagccc gtggctagat gcatcatccc tgatctgatc ggaatgggta 1020

961 agcacggagt gtagctcggg caccgatcta cgtagtaggg actagactag ccttacccat 1020

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 agtccggcaa gagcgggaat ggctcatatc gcctcctgga tcactacaag tacctcaccg 1080

1021 tcaggccgtt ctcgccctta ccgagtatag cggaggacct agtgatgttc atggagtggc 1080

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 cttggttcga gctgctgaac cttccaaaga aaatcatctt tgtgggccac gactgggggg 1140

1081 gaaccaagct cgacgacttg gaaggtttct tttagtagaa acacccggtg ctgacccccc 1140

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 cttgtctggc ctttcactac tcctacgagc accaagacaa gatcaaggcc atcgtccatg 1200

1141 gaacagaccg gaaagtgatg aggatgctcg tggttctgtt ctagttccgg tagcaggtac 1200

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... ......... EcoRV(1259)

1201 ctgagagtgt cgtggacgtg atcgagtcct gggacgagtg gcctgacatc gaggaggata 1260

1201 gactctcaca gcacctgcac tagctcagga ccctgctcac cggactgtag ctcctcctat 1260

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 tcgccctgat caagagcgaa gagggcgaga aaatggtgct tgagaataac ttcttcgtcg 1320

1261 agcgggacta gttctcgctt ctcccgctct tttaccacga actcttattg aagaagcagc 1320

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 agaccatgct cccaagcaag atcatgcgga aactggagcc tgaggagttc gctgcctacc 1380

1321 tctggtacga gggttcgttc tagtacgcct ttgacctcgg actcctcaag cgacggatgg 1380

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 tggagccatt caaggagaag ggcgaggtta gacggcctac cctctcctgg cctcgcgaga 1440

1381 acctcggtaa gttcctcttc ccgctccaat ctgccggatg ggagaggacc ggagcgctct 1440

.......... .......... ORF 3 .......... .......... ..........

.......... .......... ......... AatII(1471) ......... ..........

1441 tccctctcgt taagggaggc aagcccgacg tcgtccagat tgtccgcaac tacaacgcct 1500

1441 agggagagca attccctccg ttcgggctgc agcaggtcta acaggcgttg atgttgcgga 1500

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 accttcgggc cagcgacgat ctgcctaaga tgttcatcga gtccgaccct gggttctttt 1560

1501 tggaagcccg gtcgctgcta gacggattct acaagtagct caggctggga cccaagaaaa 1560

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 ccaacgctat tgtcgaggga gctaagaagt tccctaacac cgagttcgtg aaggtgaagg 1620

1561 ggttgcgata acagctccct cgattcttca agggattgtg gctcaagcac ttccacttcc 1620

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gcctccactt cagccaggag gacgctccag atgaaatggg taagtacatc aagagcttcg 1680

1621 cggaggtgaa gtcggtcctc ctgcgaggtc tactttaccc attcatgtag ttctcgaagc 1680

.......... .......... ORF 3 .......... .......... ..........

.......... ......... XbaI(1711) NotI(1718),EagI(1718) .........

1681 tggagcgcgt gctgaagaac gagcagtaat tctagagcgg ccgcttcgag cagacatgat 1740

1681 acctcgcgca cgacttcttg ctcgtcatta agatctcgcc ggcgaagctc gtctgtacta 1740

.......... ORF 3 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat 1800

1741 ttctatgtaa ctactcaaac ctgtttggtg ttgatcttac gtcacttttt ttacgaaata 1800

........ EBV_rev_pri SV40_PA_term ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1801 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt 1860

1801 aacactttaa acactacgat aacgaaataa acattggtaa tattcgacgt tatttgttca 1860

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt 1920

1861 attgttgttg ttaacgtaag taaaatacaa agtccaagtc cccctccaca ccctccaaaa 1920

.......... .......... .......... .......... .......... ..........

.......... .......... ......... ClaI(1956) BamHI(1963) .........

1921 ttaaagcaag taaaacctct acaaatgtgg taaaatcgat aaggatccag gtggcacttt 1980

1921 aatttcgttc attttggaga tgtttacacc attttagcta ttcctaggtc caccgtgaaa 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta 2040

1981 agccccttta cacgcgcctt ggggataaac aaataaaaag atttatgtaa gtttatacat 2040

.......... .......... .......... .......... .......... AmpR_pro

.......... .......... .......... .......... .......... ..........

2041 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat 2100

2041 aggcgagtac tctgttattg ggactattta cgaagttatt ataacttttt ccttctcata 2100

AmpR_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt 2160

2101 ctcataagtt gtaaaggcac agcgggaata agggaaaaaa cgccgtaaaa cggaaggaca 2160

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg 2220

2161 aaaacgagtg ggtctttgcg accactttca ttttctacga cttctagtca acccacgtgc 2220

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga 2280

2221 tcacccaatg tagcttgacc tagagttgtc gccattctag gaactctcaa aagcggggct 2280

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg 2340

2281 tcttgcaaaa ggttactact cgtgaaaatt tcaagacgat acaccgcgcc ataatagggc 2340

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt 2400

2341 ataactgcgg cccgttctcg ttgagccagc ggcgtatgtg ataagagtct tactgaacca 2400

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg 2460

2401 actcatgagt ggtcagtgtc ttttcgtaga atgcctaccg tactgtcatt ctcttaatac 2460

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg 2520

2461 gtcacgacgg tattggtact cactattgtg acgccggttg aatgaagact gttgctagcc 2520

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga 2580

2521 tcctggcttc ctcgattggc gaaaaaacgt gttgtacccc ctagtacatt gagcggaact 2580

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc 2640

2581 agcaaccctt ggcctcgact tacttcggta tggtttgctg ctcgcactgt ggtgctacgg 2640

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc 2700

2641 acatcgttac cgttgttgca acgcgtttga taattgaccg cttgatgaat gagatcgaag 2700

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... AseI(2711) .......... .......... .......... ..........

2701 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc 2760

2701 ggccgttgtt aattatctga cctacctccg cctatttcaa cgtcctggtg aagacgcgag 2760

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg 2820

2761 ccgggaaggc cgaccgacca aataacgact atttagacct cggccactcg cacccagagc 2820

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac 2880

2821 gccatagtaa cgtcgtgacc ccggtctacc attcgggagg gcatagcatc aatagatgtg 2880

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc 2940

2881 ctgcccctca gtccgttgat acctacttgc tttatctgtc tagcgactct atccacggag 2940

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt 3000

2941 tgactaattc gtaaccattg acagtctggt tcaaatgagt atatatgaaa tctaactaaa 3000

ORF 2 Ampicillin .... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac 3060

3001 ttttgaagta aaaattaaat tttcctagat ccacttctag gaaaaactat tagagtactg 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa 3120

3061 gttttaggga attgcactca aaagcaaggt gactcgcagt ctggggcatc ttttctagtt 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc 3180

3121 tcctagaaga actctaggaa aaaaagacgc gcattagacg acgaacgttt gtttttttgg 3180

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3181 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt 3240

3181 tggcgatggt cgccaccaaa caaacggcct agttctcgat ggttgagaaa aaggcttcca 3240

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3241 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg 3300

3241 ttgaccgaag tcgtctcgcg tctatggttt atgacaagaa gatcacatcg gcatcaatcc 3300

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3301 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc 3360

3301 ggtggtgaag ttcttgagac atcgtggcgg atgtatggag cgagacgatt aggacaatgg 3360

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3361 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt 3420

3361 tcaccgacga cggtcaccgc tattcagcac agaatggccc aacctgagtt ctgctatcaa 3420

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3421 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga 3480

3421 tggcctattc cgcgtcgcca gcccgacttg ccccccaagc acgtgtgtcg ggtcgaacct 3480

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3481 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct 3540

3481 cgcttgctgg atgtggcttg actctatgga tgtcgcactc gatactcttt cgcggtgcga 3540

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3541 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg 3600

3541 agggcttccc tctttccgcc tgtccatagg ccattcgccg tcccagcctt gtcctctcgc 3600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3601 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca 3660

3601 gtgctccctc gaaggtcccc ctttgcggac catagaaata tcaggacagc ccaaagcggt 3660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa 3720

3661 ggagactgaa ctcgcagcta aaaacactac gagcagtccc cccgcctcgg ataccttttt 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatggc 3780

3721 gcggtcgttg cgccggaaaa atgccaagga ccggaaaacg accggaaaac gagtgtaccg 3780

pBR322_origin ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 tcgac..... .......... .......... .......... .......... .......... 3785

3781 agctg..... .......... .......... .......... .......... .......... 3785

.......... .......... .......... .......... .......... ..........