SSGLOCUS phRL-TK 4045 bp DNA SYN 23-Aug-2007 DEFINITION phRL-TK ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..4045 /organism="phRL-TK" /mol_type="other DNA" promoter complement(532..680) /label="TATA_promoter" promoter 543..758 /label="TK_promoter" misc_feature 829..961 /label="chim_int" promoter 1006..1024 /label="T7_promoter" CDS 1034..1969 /label="ORF frame 2" /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAE NAVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLT AWFELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIE EDIALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLS WPREIPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNT EFVKVKGLHFSQEDAPDEMGKYIKSFVERVLKNEQ*" misc_feature complement(2011..2030) /label="EBV_rev_primer" terminator complement(1997..2116) /label="SV40_PA_terminator" promoter 2289..2317 /label="AmpR_promoter" CDS 2359..3219 /label="Ampicillin" /gene="Ampicillin" /note="ORF frame 1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" gene 2359..3219 /label="Ampicillin" /gene="Ampicillin" rep_origin 3374..3993 /label="pBR322_origin" ORIGIN 1 AGATCTAAAT GAGTCTTCGG ACCTCGCGGG GGCCGCTTAA GCGGTGGTTA GGGTTTGTCT 61 GACGCGGGGG GAGGGGGAAG GAACGAAACA CTCTCATTCG GAGGCGGCTC GGGGTTTGGT 121 CTTGGTGGCC ACGGGCACGC AGAAGAGCGC CGCGATCCTC TTAAGCACCC CCCCGCCCTC 181 CGTGGAGGCG GGGGTTTGGT CGGCGGGTGG TAACTGGCGG GCCGCTGACT CGGGCGGGTC 241 GCGCGCCCCA GAGTGTGACC TTTTCGGTCT GCTCGCAGAC CCCCGGGCGG CGCCGCCGCG 301 GCGGCGACGG GCTCGCTGGG TCCTAGGCTC CATGGGGACC GTATACGTGG ACAGGCTCTG 361 GAGCATCCGC ACGACTGCGG TGATATTACC GGAGACCTTC TGCGGGACGA GCCGGGTCAC 421 GCGGCTGACG CGGAGCGTCC GTTGGGCGAC AAACACCAGG ACGGGGCACA GGTACACTAT 481 CTTGTCACCC GGAGGCGCGA GGGACTGCAG GAGCTTCAGG GAGTGGCGCA GCTGCTTCAT 541 CCCCGTGGCC CGTTGCTCGC GTTTGCTGGC GGTGTCCCCG GAAGAAATAT ATTTGCATGT 601 CTTTAGTTCT ATGATGACAC AAACCCCGCC CAGCGTCTTG TCATTGGCGA ATTCGAACAC 661 GCAGATGCAG TCGGGGCGGC GCGGTCCCAG GTCCACTTCG CATATTAAGG TGACGCGTGT 721 GGCCTCGAAC ACCGAGCGAC CCTGCAGCGA CCCGCTTAAA AGCTTGATTC TTCTGACACA 781 ACAGTCTCGA ACTTAAGCTG CAGAAGTTGG TCGTGAGGCA CTGGGCAGGT AAGTATCAAG 841 GTTACAAGAC AGGTTTAAGG AGACCAATAG AAACTGGGCT TGTCGAGACA GAGAAGACTC 901 TTGCGTTTCT GATAGGCACC TATTGGTCTT ACTGACATCC ACTTTGCCTT TCTCTCCACA 961 GGTGTCCACT CCCAGTTCAA TTACAGCTCT TAAGGCTAGA GTACTTAATA CGACTCACTA 1021 TAGGCTAGCC ACCATGGCTT CCAAGGTGTA CGACCCCGAG CAACGCAAAC GCATGATCAC 1081 TGGGCCTCAG TGGTGGGCTC GCTGCAAGCA AATGAACGTG CTGGACTCCT TCATCAACTA 1141 CTATGATTCC GAGAAGCACG CCGAGAACGC CGTGATTTTT CTGCATGGTA ACGCTGCCTC 1201 CAGCTACCTG TGGAGGCACG TCGTGCCTCA CATCGAGCCC GTGGCTAGAT GCATCATCCC 1261 TGATCTGATC GGAATGGGTA AGTCCGGCAA GAGCGGGAAT GGCTCATATC GCCTCCTGGA 1321 TCACTACAAG TACCTCACCG CTTGGTTCGA GCTGCTGAAC CTTCCAAAGA AAATCATCTT 1381 TGTGGGCCAC GACTGGGGGG CTTGTCTGGC CTTTCACTAC TCCTACGAGC ACCAAGACAA 1441 GATCAAGGCC ATCGTCCATG CTGAGAGTGT CGTGGACGTG ATCGAGTCCT GGGACGAGTG 1501 GCCTGACATC GAGGAGGATA TCGCCCTGAT CAAGAGCGAA GAGGGCGAGA AAATGGTGCT 1561 TGAGAATAAC TTCTTCGTCG AGACCATGCT CCCAAGCAAG ATCATGCGGA AACTGGAGCC 1621 TGAGGAGTTC GCTGCCTACC TGGAGCCATT CAAGGAGAAG GGCGAGGTTA GACGGCCTAC 1681 CCTCTCCTGG CCTCGCGAGA TCCCTCTCGT TAAGGGAGGC AAGCCCGACG TCGTCCAGAT 1741 TGTCCGCAAC TACAACGCCT ACCTTCGGGC CAGCGACGAT CTGCCTAAGA TGTTCATCGA 1801 GTCCGACCCT GGGTTCTTTT CCAACGCTAT TGTCGAGGGA GCTAAGAAGT TCCCTAACAC 1861 CGAGTTCGTG AAGGTGAAGG GCCTCCACTT CAGCCAGGAG GACGCTCCAG ATGAAATGGG 1921 TAAGTACATC AAGAGCTTCG TGGAGCGCGT GCTGAAGAAC GAGCAGTAAT TCTAGAGCGG 1981 CCGCTTCGAG CAGACATGAT AAGATACATT GATGAGTTTG GACAAACCAC AACTAGAATG 2041 CAGTGAAAAA AATGCTTTAT TTGTGAAATT TGTGATGCTA TTGCTTTATT TGTAACCATT 2101 ATAAGCTGCA ATAAACAAGT TAACAACAAC AATTGCATTC ATTTTATGTT TCAGGTTCAG 2161 GGGGAGGTGT GGGAGGTTTT TTAAAGCAAG TAAAACCTCT ACAAATGTGG TAAAATCGAT 2221 AAGGATCCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC 2281 TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA 2341 TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT 2401 GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT 2461 GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC 2521 CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA 2581 TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC 2641 TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC 2701 ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC 2761 TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG 2821 GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC 2881 GAGCGTGACA CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC 2941 GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT 3001 GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA 3061 GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC 3121 CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG 3181 ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA 3241 TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC 3301 CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA 3361 GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC 3421 TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA 3481 CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT 3541 CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC 3601 GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG 3661 TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG 3721 TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG 3781 CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC 3841 AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT 3901 AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG 3961 GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC 4021 TGGCCTTTTG CTCACATGGC TCGAC // phRL-TK "Times New RomanSCTATA_pro"Times New RomanSCTK_pro"Times New RomanSCɆ=chim_int"Times New RomanST7_pro"Times New RomanSC ORF 2"Times New RomanSCɆD SV40_PA_term"Times New RomanSC EBV_rev_pri"Times New RomanS AmpR_pro"Times New RomanSC7 ORF 1"Times New RomanSC7  Ampicillin"Times New Roman(SCɆ.  pBR322_origin"Times New Roman SBglII(1),MscI(128)"Times New Roman}s2s SSmaI(284),NarI(290),SacII(299)"Times New RomanH S PvuII(531),EcoRI(649),BstBI(653)"Times New RomanEBSS SHindIII"Times New Romanpy  SNheI"Times New Romanqqqjx SEcoRV"Times New Roman?FMM, SNruI(1695),AatII(1731)"Times New Roman4<DD_b S XbaI(1971),NotI(1978),EagI(1978)"Times New RomanRZss S!HpaI(2121),ClaI(2216),BamHI(2223)"Times New RomanI|PPTF]]F SFspI(2923),AseI(2971)"Times New Romank c