Sequence of phRL-TK

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Sequence of phRL-TK

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Sequence of phRL-TK : 4045 BP; 981 A; 1009 C; 1103 G; 952 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 agatctaaat gagtcttcgg acctcgcggg ggccgcttaa gcggtggtta gggtttgtct 60

1 tctagattta ctcagaagcc tggagcgccc ccggcgaatt cgccaccaat cccaaacaga 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 gacgcggggg gagggggaag gaacgaaaca ctctcattcg gaggcggctc ggggtttggt 120

61 ctgcgccccc ctcccccttc cttgctttgt gagagtaagc ctccgccgag ccccaaacca 120

.......... .......... .......... .......... .......... ..........

MscI(128) .......... .......... .......... .......... ..........

121 cttggtggcc acgggcacgc agaagagcgc cgcgatcctc ttaagcaccc ccccgccctc 180

121 gaaccaccgg tgcccgtgcg tcttctcgcg gcgctaggag aattcgtggg ggggcgggag 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 cgtggaggcg ggggtttggt cggcgggtgg taactggcgg gccgctgact cgggcgggtc 240

181 gcacctccgc ccccaaacca gccgcccacc attgaccgcc cggcgactga gcccgcccag 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... ..... SmaI(284) NarI(290) SacII(299)

241 gcgcgcccca gagtgtgacc ttttcggtct gctcgcagac ccccgggcgg cgccgccgcg 300

241 cgcgcggggt ctcacactgg aaaagccaga cgagcgtctg ggggcccgcc gcggcggcgc 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 gcggcgacgg gctcgctggg tcctaggctc catggggacc gtatacgtgg acaggctctg 360

301 cgccgctgcc cgagcgaccc aggatccgag gtacccctgg catatgcacc tgtccgagac 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 gagcatccgc acgactgcgg tgatattacc ggagaccttc tgcgggacga gccgggtcac 420

361 ctcgtaggcg tgctgacgcc actataatgg cctctggaag acgccctgct cggcccagtg 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 gcggctgacg cggagcgtcc gttgggcgac aaacaccagg acggggcaca ggtacactat 480

421 cgccgactgc gcctcgcagg caacccgctg tttgtggtcc tgccccgtgt ccatgtgata 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 cttgtcaccc ggaggcgcga gggactgcag gagcttcagg gagtggcgca gctgcttcat 540

481 gaacagtggg cctccgcgct ccctgacgtc ctcgaagtcc ctcaccgcgt cgacgaagta 540

.......... .......... .......... .......... .......... TATA_pro

.......... .......... .......... .......... .......... ..........

541 ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg gaagaaatat atttgcatgt 600

541 ggggcaccgg gcaacgagcg caaacgaccg ccacaggggc cttctttata taaacgtaca 600

.......... .......... TATA_pro TK_pro .......... ..........

.......... .......... .......... .......... .... EcoRI(649),BstBI(653)

601 ctttagttct atgatgacac aaaccccgcc cagcgtcttg tcattggcga attcgaacac 660

601 gaaatcaaga tactactgtg tttggggcgg gtcgcagaac agtaaccgct taagcttgtg 660

.......... ....... TATA_pro TK_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

661 gcagatgcag tcggggcggc gcggtcccag gtccacttcg catattaagg tgacgcgtgt 720

661 cgtctacgtc agccccgccg cgccagggtc caggtgaagc gtataattcc actgcgcaca 720

TATA_pro .......... TK_pro .......... .......... ..........

.......... .......... ......... HindIII(760) ......... ..........

721 ggcctcgaac accgagcgac cctgcagcga cccgcttaaa agcttgattc ttctgacaca 780

721 ccggagcttg tggctcgctg ggacgtcgct gggcgaattt tcgaactaag aagactgtgt 780

.......... TK_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 acagtctcga acttaagctg cagaagttgg tcgtgaggca ctgggcaggt aagtatcaag 840

781 tgtcagagct tgaattcgac gtcttcaacc agcactccgt gacccgtcca ttcatagttc 840

.......... .......... .......... .......... .......... chim_int

.......... .......... .......... .......... .......... ..........

841 gttacaagac aggtttaagg agaccaatag aaactgggct tgtcgagaca gagaagactc 900

841 caatgttctg tccaaattcc tctggttatc tttgacccga acagctctgt ctcttctgag 900

.......... .......... chim_int .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 ttgcgtttct gataggcacc tattggtctt actgacatcc actttgcctt tctctccaca 960

901 aacgcaaaga ctatccgtgg ataaccagaa tgactgtagg tgaaacggaa agagaggtgt 960

.......... .......... chim_int .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 ggtgtccact cccagttcaa ttacagctct taaggctaga gtacttaata cgactcacta 1020

961 ccacaggtga gggtcaagtt aatgtcgaga attccgatct catgaattat gctgagtgat 1020

.......... .......... .......... .......... .......... T7_pro

NheI(1024) .......... .......... .......... .......... ..........

1021 taggctagcc accatggctt ccaaggtgta cgaccccgag caacgcaaac gcatgatcac 1080

1021 atccgatcgg tggtaccgaa ggttccacat gctggggctc gttgcgtttg cgtactagtg 1080

.......... .......... .......... ORF 2 .......... ..........

.......... .......... .......... .......... .......... ..........

1081 tgggcctcag tggtgggctc gctgcaagca aatgaacgtg ctggactcct tcatcaacta 1140

1081 acccggagtc accacccgag cgacgttcgt ttacttgcac gacctgagga agtagttgat 1140

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 ctatgattcc gagaagcacg ccgagaacgc cgtgattttt ctgcatggta acgctgcctc 1200

1141 gatactaagg ctcttcgtgc ggctcttgcg gcactaaaaa gacgtaccat tgcgacggag 1200

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 cagctacctg tggaggcacg tcgtgcctca catcgagccc gtggctagat gcatcatccc 1260

1201 gtcgatggac acctccgtgc agcacggagt gtagctcggg caccgatcta cgtagtaggg 1260

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 tgatctgatc ggaatgggta agtccggcaa gagcgggaat ggctcatatc gcctcctgga 1320

1261 actagactag ccttacccat tcaggccgtt ctcgccctta ccgagtatag cggaggacct 1320

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 tcactacaag tacctcaccg cttggttcga gctgctgaac cttccaaaga aaatcatctt 1380

1321 agtgatgttc atggagtggc gaaccaagct cgacgacttg gaaggtttct tttagtagaa 1380

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 tgtgggccac gactgggggg cttgtctggc ctttcactac tcctacgagc accaagacaa 1440

1381 acacccggtg ctgacccccc gaacagaccg gaaagtgatg aggatgctcg tggttctgtt 1440

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 gatcaaggcc atcgtccatg ctgagagtgt cgtggacgtg atcgagtcct gggacgagtg 1500

1441 ctagttccgg tagcaggtac gactctcaca gcacctgcac tagctcagga ccctgctcac 1500

.......... .......... ORF 2 .......... .......... ..........

......... EcoRV(1519) ......... .......... .......... ..........

1501 gcctgacatc gaggaggata tcgccctgat caagagcgaa gagggcgaga aaatggtgct 1560

1501 cggactgtag ctcctcctat agcgggacta gttctcgctt ctcccgctct tttaccacga 1560

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 tgagaataac ttcttcgtcg agaccatgct cccaagcaag atcatgcgga aactggagcc 1620

1561 actcttattg aagaagcagc tctggtacga gggttcgttc tagtacgcct ttgacctcgg 1620

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 tgaggagttc gctgcctacc tggagccatt caaggagaag ggcgaggtta gacggcctac 1680

1621 actcctcaag cgacggatgg acctcggtaa gttcctcttc ccgctccaat ctgccggatg 1680

.......... .......... ORF 2 .......... .......... ..........

.......... NruI(1695) .......... .......... ......... AatII(1731)

1681 cctctcctgg cctcgcgaga tccctctcgt taagggaggc aagcccgacg tcgtccagat 1740

1681 ggagaggacc ggagcgctct agggagagca attccctccg ttcgggctgc agcaggtcta 1740

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 tgtccgcaac tacaacgcct accttcgggc cagcgacgat ctgcctaaga tgttcatcga 1800

1741 acaggcgttg atgttgcgga tggaagcccg gtcgctgcta gacggattct acaagtagct 1800

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 gtccgaccct gggttctttt ccaacgctat tgtcgaggga gctaagaagt tccctaacac 1860

1801 caggctggga cccaagaaaa ggttgcgata acagctccct cgattcttca agggattgtg 1860

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 cgagttcgtg aaggtgaagg gcctccactt cagccaggag gacgctccag atgaaatggg 1920

1861 gctcaagcac ttccacttcc cggaggtgaa gtcggtcctc ctgcgaggtc tactttaccc 1920

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... ......... XbaI(1971) NotI(1978),EagI(1978)

1921 taagtacatc aagagcttcg tggagcgcgt gctgaagaac gagcagtaat tctagagcgg 1980

1921 attcatgtag ttctcgaagc acctcgcgca cgacttcttg ctcgtcatta agatctcgcc 1980

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 ccgcttcgag cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg 2040

1981 ggcgaagctc gtctgtacta ttctatgtaa ctactcaaac ctgtttggtg ttgatcttac 2040

.......... .......... ... SV40_PA_term EBV_rev_pri ... ..........

.......... .......... .......... .......... .......... ..........

2041 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt 2100

2041 gtcacttttt ttacgaaata aacactttaa acactacgat aacgaaataa acattggtaa 2100

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag 2160

2101 tattcgacgt tatttgttca attgttgttg ttaacgtaag taaaatacaa agtccaagtc 2160

SV40_PA_term ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg taaaatcgat 2220

2161 cccctccaca ccctccaaaa aatttcgttc attttggaga tgtttacacc attttagcta 2220

.......... .......... .......... .......... .......... ..........

BamHI(2223) ........ .......... .......... .......... ..........

2221 aaggatccag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc 2280

2221 ttcctaggtc caccgtgaaa agccccttta cacgcgcctt ggggataaac aaataaaaag 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa 2340

2281 atttatgtaa gtttatacat aggcgagtac tctgttattg ggactattta cgaagttatt 2340

.......... .......... AmpR_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt 2400

2341 ataacttttt ccttctcata ctcataagtt gtaaaggcac agcgggaata agggaaaaaa 2400

.......... .......... ....... ORF 1 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

2401 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct 2460

2401 cgccgtaaaa cggaaggaca aaaacgagtg ggtctttgcg accactttca ttttctacga 2460

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc 2520

2461 cttctagtca acccacgtgc tcacccaatg tagcttgacc tagagttgtc gccattctag 2520

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta 2580

2521 gaactctcaa aagcggggct tcttgcaaaa ggttactact cgtgaaaatt tcaagacgat 2580

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac 2640

2581 acaccgcgcc ataatagggc ataactgcgg cccgttctcg ttgagccagc ggcgtatgtg 2640

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc 2700

2641 ataagagtct tactgaacca actcatgagt ggtcagtgtc ttttcgtaga atgcctaccg 2700

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac 2760

2701 tactgtcatt ctcttaatac gtcacgacgg tattggtact cactattgtg acgccggttg 2760

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg 2820

2761 aatgaagact gttgctagcc tcctggcttc ctcgattggc gaaaaaacgt gttgtacccc 2820

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac 2880

2821 ctagtacatt gagcggaact agcaaccctt ggcctcgact tacttcggta tggtttgctg 2880

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc 2940

2881 ctcgcactgt ggtgctacgg acatcgttac cgttgttgca acgcgtttga taattgaccg 2940

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... AseI(2971) .......... ..........

2941 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt 3000

2941 cttgatgaat gagatcgaag ggccgttgtt aattatctga cctacctccg cctatttcaa 3000

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga 3060

3001 cgtcctggtg aagacgcgag ccgggaaggc cgaccgacca aataacgact atttagacct 3060

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc 3120

3061 cggccactcg cacccagagc gccatagtaa cgtcgtgacc ccggtctacc attcgggagg 3120

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag 3180

3121 gcatagcatc aatagatgtg ctgcccctca gtccgttgat acctacttgc tttatctgtc 3180

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca 3240

3181 tagcgactct atccacggag tgactaattc gtaaccattg acagtctggt tcaaatgagt 3240

....... ORF 1 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc 3300

3241 atatatgaaa tctaactaaa ttttgaagta aaaattaaat tttcctagat ccacttctag 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca 3360

3301 gaaaaactat tagagtactg gttttaggga attgcactca aaagcaaggt gactcgcagt 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc 3420

3361 ctggggcatc ttttctagtt tcctagaaga actctaggaa aaaaagacgc gcattagacg 3420

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

3421 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta 3480

3421 acgaacgttt gtttttttgg tggcgatggt cgccaccaaa caaacggcct agttctcgat 3480

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3481 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt 3540

3481 ggttgagaaa aaggcttcca ttgaccgaag tcgtctcgcg tctatggttt atgacaagaa 3540

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3541 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc 3600

3541 gatcacatcg gcatcaatcc ggtggtgaag ttcttgagac atcgtggcgg atgtatggag 3600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3601 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg 3660

3601 cgagacgatt aggacaatgg tcaccgacga cggtcaccgc tattcagcac agaatggccc 3660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg 3720

3661 aacctgagtt ctgctatcaa tggcctattc cgcgtcgcca gcccgacttg ccccccaagc 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag 3780

3721 acgtgtgtcg ggtcgaacct cgcttgctgg atgtggcttg actctatgga tgtcgcactc 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc 3840

3781 gatactcttt cgcggtgcga agggcttccc tctttccgcc tgtccatagg ccattcgccg 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat 3900

3841 tcccagcctt gtcctctcgc gtgctccctc gaaggtcccc ctttgcggac catagaaata 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg 3960

3901 tcaggacagc ccaaagcggt ggagactgaa ctcgcagcta aaaacactac gagcagtccc 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc 4020

3961 cccgcctcgg ataccttttt gcggtcgttg cgccggaaaa atgccaagga ccggaaaacg 4020

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 tggccttttg ctcacatggc tcgac..... .......... .......... .......... 4045

4021 accggaaaac gagtgtaccg agctg..... .......... .......... .......... 4045

.......... .......... .......... .......... .......... ..........