Sequence of phRL-null

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Sequence of phRL-null

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Sequence of phRL-null : 3320 BP; 859 A; 798 C; 847 G; 816 T; 0 other;

BglII,XhoI SacI,HindIII NdeI SpeI NarI1) SalI(47)

1 agatctcgag ctctaagctt cacatatgca tgcactagtg gcgcctgtcg acgcgtagaa 60

1 tctagagctc gagattcgaa gtgtatacgt acgtgatcac cgcggacagc tgcgcatctt 60

.......... .......... .......... .......... .......... ..........

SmaI(67) PstI(77) .......... .......... .......... ..........

61 ttcacccggg tactgcagaa gttggtcgtg aggcactggg caggtaagta tcaaggttac 120

61 aagtgggccc atgacgtctt caaccagcac tccgtgaccc gtccattcat agttccaatg 120

.......... .......... .......... .......... .......... chim_int

.......... .......... .......... .......... .......... ..........

121 aagacaggtt taaggagacc aatagaaact gggcttgtcg agacagagaa gactcttgcg 180

121 ttctgtccaa attcctctgg ttatctttga cccgaacagc tctgtctctt ctgagaacgc 180

.......... .......... chim_int .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 tttctgatag gcacctattg gtcttactga catccacttt gcctttctct ccacaggtgt 240

181 aaagactatc cgtggataac cagaatgact gtaggtgaaa cggaaagaga ggtgtccaca 240

.......... .......... chim_int .......... .......... ..........

.......... .......... AflII(264) .......... .......... ..........

241 ccactcccag ttcaattaca gctcttaagg ctagagtact taatacgact cactataggc 300

241 ggtgagggtc aagttaatgt cgagaattcc gatctcatga attatgctga gtgatatccg 300

.......... .......... .......... .......... T7_pro ..........

NcoI(307) .......... .......... .......... .......... ..........

301 tagccaccat ggcttccaag gtgtacgacc ccgagcaacg caaacgcatg atcactgggc 360

301 atcggtggta ccgaaggttc cacatgctgg ggctcgttgc gtttgcgtac tagtgacccg 360

.......... .......... .......... ORF 3 .......... ..........

.......... .......... .......... .......... .......... ..........

361 ctcagtggtg ggctcgctgc aagcaaatga acgtgctgga ctccttcatc aactactatg 420

361 gagtcaccac ccgagcgacg ttcgtttact tgcacgacct gaggaagtag ttgatgatac 420

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 attccgagaa gcacgccgag aacgccgtga tttttctgca tggtaacgct gcctccagct 480

421 taaggctctt cgtgcggctc ttgcggcact aaaaagacgt accattgcga cggaggtcga 480

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 acctgtggag gcacgtcgtg cctcacatcg agcccgtggc tagatgcatc atccctgatc 540

481 tggacacctc cgtgcagcac ggagtgtagc tcgggcaccg atctacgtag tagggactag 540

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 tgatcggaat gggtaagtcc ggcaagagcg ggaatggctc atatcgcctc ctggatcact 600

541 actagcctta cccattcagg ccgttctcgc ccttaccgag tatagcggag gacctagtga 600

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 acaagtacct caccgcttgg ttcgagctgc tgaaccttcc aaagaaaatc atctttgtgg 660

601 tgttcatgga gtggcgaacc aagctcgacg acttggaagg tttcttttag tagaaacacc 660

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 gccacgactg gggggcttgt ctggcctttc actactccta cgagcaccaa gacaagatca 720

661 cggtgctgac cccccgaaca gaccggaaag tgatgaggat gctcgtggtt ctgttctagt 720

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 aggccatcgt ccatgctgag agtgtcgtgg acgtgatcga gtcctgggac gagtggcctg 780

721 tccggtagca ggtacgactc tcacagcacc tgcactagct caggaccctg ctcaccggac 780

.......... .......... ORF 3 .......... .......... ..........

.......... EcoRV(794) .......... .......... .......... ..........

781 acatcgagga ggatatcgcc ctgatcaaga gcgaagaggg cgagaaaatg gtgcttgaga 840

781 tgtagctcct cctatagcgg gactagttct cgcttctccc gctcttttac cacgaactct 840

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ataacttctt cgtcgagacc atgctcccaa gcaagatcat gcggaaactg gagcctgagg 900

841 tattgaagaa gcagctctgg tacgagggtt cgttctagta cgcctttgac ctcggactcc 900

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 agttcgctgc ctacctggag ccattcaagg agaagggcga ggttagacgg cctaccctct 960

901 tcaagcgacg gatggacctc ggtaagttcc tcttcccgct ccaatctgcc ggatgggaga 960

.......... .......... ORF 3 .......... .......... ..........

NruI(970) .......... .......... ......... AatII(1006) .........

961 cctggcctcg cgagatccct ctcgttaagg gaggcaagcc cgacgtcgtc cagattgtcc 1020

961 ggaccggagc gctctaggga gagcaattcc ctccgttcgg gctgcagcag gtctaacagg 1020

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 gcaactacaa cgcctacctt cgggccagcg acgatctgcc taagatgttc atcgagtccg 1080

1021 cgttgatgtt gcggatggaa gcccggtcgc tgctagacgg attctacaag tagctcaggc 1080

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 accctgggtt cttttccaac gctattgtcg agggagctaa gaagttccct aacaccgagt 1140

1081 tgggacccaa gaaaaggttg cgataacagc tccctcgatt cttcaaggga ttgtggctca 1140

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 tcgtgaaggt gaagggcctc cacttcagcc aggaggacgc tccagatgaa atgggtaagt 1200

1141 agcacttcca cttcccggag gtgaagtcgg tcctcctgcg aggtctactt tacccattca 1200

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .... XbaI(1246) NotI(1253),EagI(1253)

1201 acatcaagag cttcgtggag cgcgtgctga agaacgagca gtaattctag agcggccgct 1260

1201 tgtagttctc gaagcacctc gcgcacgact tcttgctcgt cattaagatc tcgccggcga 1260

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 tcgagcagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg 1320

1261 agctcgtctg tactattcta tgtaactact caaacctgtt tggtgttgat cttacgtcac 1320

.......... .......... ... SV40_PA_term EBV_rev_pri ... ..........

.......... .......... .......... .......... .......... ..........

1321 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag 1380

1321 tttttttacg aaataaacac tttaaacact acgataacga aataaacatt ggtaatattc 1380

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga 1440

1381 gacgttattt gttcaattgt tgttgttaac gtaagtaaaa tacaaagtcc aagtccccct 1440

SV40_PA_term ........ .......... .......... .......... ..........

.......... .......... .......... .......... .... ClaI(1491) BamHI(1498)

1441 ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa tcgataagga 1500

1441 ccacaccctc caaaaaattt cgttcatttt ggagatgttt acaccatttt agctattcct 1500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 tccaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat 1560

1501 aggtccaccg tgaaaagccc ctttacacgc gccttgggga taaacaaata aaaagattta 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg 1620

1561 tgtaagttta tacataggcg agtactctgt tattgggact atttacgaag ttattataac 1620

.......... AmpR_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc 1680

1621 tttttccttc tcatactcat aagttgtaaa ggcacagcgg gaataaggga aaaaacgccg 1680

.......... .......... ....... ORF 2 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

1681 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga 1740

1681 taaaacggaa ggacaaaaac gagtgggtct ttgcgaccac tttcattttc tacgacttct 1740

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga 1800

1741 agtcaaccca cgtgctcacc caatgtagct tgacctagag ttgtcgccat tctaggaact 1800

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg 1860

1801 ctcaaaagcg gggcttcttg caaaaggtta ctactcgtga aaatttcaag acgatacacc 1860

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc 1920

1861 gcgccataat agggcataac tgcggcccgt tctcgttgag ccagcggcgt atgtgataag 1920

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac 1980

1921 agtcttactg aaccaactca tgagtggtca gtgtcttttc gtagaatgcc taccgtactg 1980

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact 2040

1981 tcattctctt aatacgtcac gacggtattg gtactcacta ttgtgacgcc ggttgaatga 2040

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca 2100

2041 agactgttgc tagcctcctg gcttcctcga ttggcgaaaa aacgtgttgt accccctagt 2100

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg 2160

2101 acattgagcg gaactagcaa cccttggcct cgacttactt cggtatggtt tgctgctcgc 2160

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact 2220

2161 actgtggtgc tacggacatc gttaccgttg ttgcaacgcg tttgataatt gaccgcttga 2220

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... AseI(2246) .......... .......... ..........

2221 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg 2280

2221 tgaatgagat cgaagggccg ttgttaatta tctgacctac ctccgcctat ttcaacgtcc 2280

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg 2340

2281 tggtgaagac gcgagccggg aaggccgacc gaccaaataa cgactattta gacctcggcc 2340

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat 2400

2341 actcgcaccc agagcgccat agtaacgtcg tgaccccggt ctaccattcg ggagggcata 2400

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc 2460

2401 gcatcaatag atgtgctgcc cctcagtccg ttgataccta cttgctttat ctgtctagcg 2460

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat 2520

2461 actctatcca cggagtgact aattcgtaac cattgacagt ctggttcaaa tgagtatata 2520

....... ORF 2 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt 2580

2521 tgaaatctaa ctaaattttg aagtaaaaat taaattttcc tagatccact tctaggaaaa 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc 2640

2581 actattagag tactggtttt agggaattgc actcaaaagc aaggtgactc gcagtctggg 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt 2700

2641 gcatcttttc tagtttccta gaagaactct aggaaaaaaa gacgcgcatt agacgacgaa 2700

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

2701 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac 2760

2701 cgtttgtttt tttggtggcg atggtcgcca ccaaacaaac ggcctagttc tcgatggttg 2760

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2761 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt 2820

2761 agaaaaaggc ttccattgac cgaagtcgtc tcgcgtctat ggtttatgac aagaagatca 2820

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2821 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct 2880

2821 catcggcatc aatccggtgg tgaagttctt gagacatcgt ggcggatgta tggagcgaga 2880

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga 2940

2881 cgattaggac aatggtcacc gacgacggtc accgctattc agcacagaat ggcccaacct 2940

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2941 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac 3000

2941 gagttctgct atcaatggcc tattccgcgt cgccagcccg acttgccccc caagcacgtg 3000

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3001 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg 3060

3001 tgtcgggtcg aacctcgctt gctggatgtg gcttgactct atggatgtcg cactcgatac 3060

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3061 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt 3120

3061 tctttcgcgg tgcgaagggc ttccctcttt ccgcctgtcc ataggccatt cgccgtccca 3120

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3121 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc 3180

3121 gccttgtcct ctcgcgtgct ccctcgaagg tccccctttg cggaccatag aaatatcagg 3180

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3181 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg 3240

3181 acagcccaaa gcggtggaga ctgaactcgc agctaaaaac actacgagca gtccccccgc 3240

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3241 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc 3300

3241 ctcggatacc tttttgcggt cgttgcgccg gaaaaatgcc aaggaccgga aaacgaccgg 3300

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 ttttgctcac atggctcgac .......... .......... .......... .......... 3320

3301 aaaacgagtg taccgagctg .......... .......... .......... .......... 3320

.......... .......... .......... .......... .......... ..........