aaLOCUS phrG-B 4101 bp DNA SYN 07-Sep-2007 DEFINITION phrG-B ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..4101 /organism="phrG-B" /mol_type="other DNA" CDS 88..1023 /label="ORF frame 1" /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAE NAVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLT AWFELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIE EDIALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLS WPREIPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNT EFVKVKGLHFSQEDAPDEMGKYIKSFVERVLKNEQ*" misc_feature complement(1075..1094) /label="EBV_rev_primer" terminator complement(1061..1180) /label="SV40_PA_terminator" rep_origin complement(1589..2208) /label="pBR322_origin" CDS complement(2363..3223) /label="Ampicillin" /gene="Ampicillin" /note="ORF frame 2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" gene complement(2363..3223) /label="Ampicillin" /gene="Ampicillin" promoter complement(3265..3293) /label="AmpR_promoter" rep_origin 3372..3678 /label="f1_origin" terminator 3941..3989 /label="synth_PA_terminator" misc_feature 3941..4094 /label="TB" terminator 3941..4094 /label="up_PA_terminator" misc_feature 4043..4062 /label="RVprimer3_primer" ORIGIN 1 GGTACCGAGC TCTTACGCGT GCTAGCCCGG GCTCGAGATC TGCGATCTAA GTAAGCTTGG 61 CATTCCGGTA CTGTTGGTAA AGCCACCATG GCTTCCAAGG TGTACGACCC CGAGCAACGC 121 AAACGCATGA TCACTGGGCC TCAGTGGTGG GCTCGCTGCA AGCAAATGAA CGTGCTGGAC 181 TCCTTCATCA ACTACTATGA TTCCGAGAAG CACGCCGAGA ACGCCGTGAT TTTTCTGCAT 241 GGTAACGCTG CCTCCAGCTA CCTGTGGAGG CACGTCGTGC CTCACATCGA GCCCGTGGCT 301 AGATGCATCA TCCCTGATCT GATCGGAATG GGTAAGTCCG GCAAGAGCGG GAATGGCTCA 361 TATCGCCTCC TGGATCACTA CAAGTACCTC ACCGCTTGGT TCGAGCTGCT GAACCTTCCA 421 AAGAAAATCA TCTTTGTGGG CCACGACTGG GGGGCTTGTC TGGCCTTTCA CTACTCCTAC 481 GAGCACCAAG ACAAGATCAA GGCCATCGTC CATGCTGAGA GTGTCGTGGA CGTGATCGAG 541 TCCTGGGACG AGTGGCCTGA CATCGAGGAG GATATCGCCC TGATCAAGAG CGAAGAGGGC 601 GAGAAAATGG TGCTTGAGAA TAACTTCTTC GTCGAGACCA TGCTCCCAAG CAAGATCATG 661 CGGAAACTGG AGCCTGAGGA GTTCGCTGCC TACCTGGAGC CATTCAAGGA GAAGGGCGAG 721 GTTAGACGGC CTACCCTCTC CTGGCCTCGC GAGATCCCTC TCGTTAAGGG AGGCAAGCCC 781 GACGTCGTCC AGATTGTCCG CAACTACAAC GCCTACCTTC GGGCCAGCGA CGATCTGCCT 841 AAGATGTTCA TCGAGTCCGA CCCTGGGTTC TTTTCCAACG CTATTGTCGA GGGAGCTAAG 901 AAGTTCCCTA ACACCGAGTT CGTGAAGGTG AAGGGCCTCC ACTTCAGCCA GGAGGACGCT 961 CCAGATGAAA TGGGTAAGTA CATCAAGAGC TTCGTGGAGC GCGTGCTGAA GAACGAGCAG 1021 TAATTCTAGA GTCGGGGCGG CCGGCCGCTT CGAGCAGACA TGATAAGATA CATTGATGAG 1081 TTTGGACAAA CCACAACTAG AATGCAGTGA AAAAAATGCT TTATTTGTGA AATTTGTGAT 1141 GCTATTGCTT TATTTGTAAC CATTATAAGC TGCAATAAAC AAGTTAACAA CAACAATTGC 1201 ATTCATTTTA TGTTTCAGGT TCAGGGGGAG GTGTGGGAGG TTTTTTAAAG CAAGTAAAAC 1261 CTCTACAAAT GTGGTAAAAT CGATAAGGAT CCGTCGACCG ATGCCCTTGA GAGCCTTCAA 1321 CCCAGTCAGC TCCTTCCGGT GGGCGCGGGG CATGACTATC GTCGCCGCAC TTATGACTGT 1381 CTTCTTTATC ATGCAACTCG TAGGACAGGT GCCGGCAGCG CTCTTCCGCT TCCTCGCTCA 1441 CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG 1501 TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC 1561 AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC 1621 CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC 1681 TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC 1741 TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA 1801 GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC 1861 ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA 1921 ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG 1981 CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA 2041 GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG 2101 GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC 2161 AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT 2221 CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA 2281 GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT 2341 ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA 2401 TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC 2461 GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG 2521 CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG 2581 CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT 2641 CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT 2701 CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT 2761 CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA 2821 AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA 2881 TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT 2941 AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC 3001 ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA 3061 GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT 3121 CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG 3181 CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT 3241 ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT 3301 AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC 3361 CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC 3421 TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG 3481 CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT 3541 TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC 3601 CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT 3661 TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA 3721 TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA 3781 ATTTTAACAA AATATTAACG CTTACAATTT GCCATTCGCC ATTCAGGCTG CGCAACTGTT 3841 GGGAAGGGCG ATCGGTGCGG GCCTCTTCGC TATTACGCCA GCCCAAGCTA CCATGATAAG 3901 TAAGTAATAT TAAGGTACGG GAGGTACTTG GAGCGGCCGC AATAAAATAT CTTTATTTTC 3961 ATTACATCTG TGTGTTGGTT TTTTGTGTGA ATCGATAGTA CTAACATACG CTCTCCATCA 4021 AAACAAAACG AAACAAAACA AACTAGCAAA ATAGGCTGTC CCCAGTGCAA GTGCAGGTGC 4081 CAGAACATTT CTCTATCGAT A // phrG-B "Times New RomanaCXORF 1"Times New RomanaCɆ% SV40_PA_term"Times New RomanaC3F EBV_rev_pri"Times New Romana5 pBR322_origin"Times New RomanaC; ORF 2"Times New RomanaC;  Ampicillin"Times New RomanaCɆ AmpR_pro"Times New Romana, ^ f1_origin"Times New RomanaCe synth_PA_term"Times New RomanaCeTB"Times New RomanaCɆe up_PA_term"Times New Roman*a RVpri3_pri"Times New Roman aJKpnI(5),SacI(11),NheI(21),SmaI(28),XhoI(32),BglII(36),HindIII(53),NcoI(86)"Times New Roman 1qu+q aEcoRV"Times New Roman=bipp aNruI(749),AatII(785)"Times New Roman aXbaI"Times New Roman{cZKZ a!HpaI(1185),BamHI(1287),SalI(1293)"Times New Roman" aAseI"Times New Roman0 !!0 aNotI"Times New Roman^9:vspQz atest"Times New Romanx!$L