Sequence of phrG-B

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Sequence of phrG-B

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Sequence of phrG-B : 4101 BP; 1022 A; 1038 C; 1011 G; 1030 T; 0 other;

KpnI SacI11) NheI(21) SmaI(28),XhoI(32),BglII(36) ..

1 ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tgcgatctaa gtaagcttgg 60

1 ccatggctcg agaatgcgca cgatcgggcc cgagctctag acgctagatt cattcgaacc 60

.......... .......... .......... .......... .......... ..........

.......... .......... NcoI(86) .......... .......... ..........

61 cattccggta ctgttggtaa agccaccatg gcttccaagg tgtacgaccc cgagcaacgc 120

61 gtaaggccat gacaaccatt tcggtggtac cgaaggttcc acatgctggg gctcgttgcg 120

.......... .......... .......... .......... ORF 1 ..........

.......... .......... .......... .......... .......... ..........

121 aaacgcatga tcactgggcc tcagtggtgg gctcgctgca agcaaatgaa cgtgctggac 180

121 tttgcgtact agtgacccgg agtcaccacc cgagcgacgt tcgtttactt gcacgacctg 180

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 tccttcatca actactatga ttccgagaag cacgccgaga acgccgtgat ttttctgcat 240

181 aggaagtagt tgatgatact aaggctcttc gtgcggctct tgcggcacta aaaagacgta 240

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 ggtaacgctg cctccagcta cctgtggagg cacgtcgtgc ctcacatcga gcccgtggct 300

241 ccattgcgac ggaggtcgat ggacacctcc gtgcagcacg gagtgtagct cgggcaccga 300

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 agatgcatca tccctgatct gatcggaatg ggtaagtccg gcaagagcgg gaatggctca 360

301 tctacgtagt agggactaga ctagccttac ccattcaggc cgttctcgcc cttaccgagt 360

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 tatcgcctcc tggatcacta caagtacctc accgcttggt tcgagctgct gaaccttcca 420

361 atagcggagg acctagtgat gttcatggag tggcgaacca agctcgacga cttggaaggt 420

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 aagaaaatca tctttgtggg ccacgactgg ggggcttgtc tggcctttca ctactcctac 480

421 ttcttttagt agaaacaccc ggtgctgacc ccccgaacag accggaaagt gatgaggatg 480

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 gagcaccaag acaagatcaa ggccatcgtc catgctgaga gtgtcgtgga cgtgatcgag 540

481 ctcgtggttc tgttctagtt ccggtagcag gtacgactct cacagcacct gcactagctc 540

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... EcoRV(573) .......... ..........

541 tcctgggacg agtggcctga catcgaggag gatatcgccc tgatcaagag cgaagagggc 600

541 aggaccctgc tcaccggact gtagctcctc ctatagcggg actagttctc gcttctcccg 600

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 gagaaaatgg tgcttgagaa taacttcttc gtcgagacca tgctcccaag caagatcatg 660

601 ctcttttacc acgaactctt attgaagaag cagctctggt acgagggttc gttctagtac 660

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 cggaaactgg agcctgagga gttcgctgcc tacctggagc cattcaagga gaagggcgag 720

661 gcctttgacc tcggactcct caagcgacgg atggacctcg gtaagttcct cttcccgctc 720

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 gttagacggc ctaccctctc ctggcctcgc gagatccctc tcgttaaggg aggcaagccc 780

721 caatctgccg gatgggagag gaccggagcg ctctagggag agcaattccc tccgttcggg 780

.......... .......... ORF 1 .......... .......... ..........

AatII(785) .......... .......... .......... .......... ..........

781 gacgtcgtcc agattgtccg caactacaac gcctaccttc gggccagcga cgatctgcct 840

781 ctgcagcagg tctaacaggc gttgatgttg cggatggaag cccggtcgct gctagacgga 840

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 aagatgttca tcgagtccga ccctgggttc ttttccaacg ctattgtcga gggagctaag 900

841 ttctacaagt agctcaggct gggacccaag aaaaggttgc gataacagct ccctcgattc 900

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 aagttcccta acaccgagtt cgtgaaggtg aagggcctcc acttcagcca ggaggacgct 960

901 ttcaagggat tgtggctcaa gcacttccac ttcccggagg tgaagtcggt cctcctgcga 960

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 ccagatgaaa tgggtaagta catcaagagc ttcgtggagc gcgtgctgaa gaacgagcag 1020

961 ggtctacttt acccattcat gtagttctcg aagcacctcg cgcacgactt cttgctcgtc 1020

.......... .......... ORF 1 .......... .......... ..........

XbaI(1025) .......... .......... .......... .......... ..........

1021 taattctaga gtcggggcgg ccggccgctt cgagcagaca tgataagata cattgatgag 1080

1021 attaagatct cagccccgcc ggccggcgaa gctcgtctgt actattctat gtaactactc 1080

.......... .......... .......... ......... SV40_PA_term .........

.......... .......... .......... .......... .......... ..........

1081 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat 1140

1081 aaacctgttt ggtgttgatc ttacgtcact ttttttacga aataaacact ttaaacacta 1140

EBV_rev_pri ....... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc 1200

1141 cgataacgaa ataaacattg gtaatattcg acgttatttg ttcaattgtt gttgttaacg 1200

......... SV40_PA_term ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac 1260

1201 taagtaaaat acaaagtcca agtccccctc cacaccctcc aaaaaatttc gttcattttg 1260

.......... .......... .......... .......... .......... ..........

.......... ......... BamHI(1287) SalI(1293) ......... ..........

1261 ctctacaaat gtggtaaaat cgataaggat ccgtcgaccg atgcccttga gagccttcaa 1320

1261 gagatgttta caccatttta gctattccta ggcagctggc tacgggaact ctcggaagtt 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac ttatgactgt 1380

1321 gggtcagtcg aggaaggcca cccgcgcccc gtactgatag cagcggcgtg aatactgaca 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 cttctttatc atgcaactcg taggacaggt gccggcagcg ctcttccgct tcctcgctca 1440

1381 gaagaaatag tacgttgagc atcctgtcca cggccgtcgc gagaaggcga aggagcgagt 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 1500

1441 gactgagcga cgcgagccag caagccgacg ccgctcgcca tagtcgagtg agtttccgcc 1500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 1560

1501 attatgccaa taggtgtctt agtcccctat tgcgtccttt cttgtacact cgttttccgg 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 1620

1561 tcgttttccg gtccttggca tttttccggc gcaacgaccg caaaaaggta tccgaggcgg 1620

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

1621 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 1680

1621 ggggactgct cgtagtgttt ttagctgcga gttcagtctc caccgctttg ggctgtcctg 1680

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1681 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 1740

1681 atatttctat ggtccgcaaa gggggacctt cgagggagca cgcgagagga caaggctggg 1740

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1741 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 1800

1741 acggcgaatg gcctatggac aggcggaaag agggaagccc ttcgcaccgc gaaagagtat 1800

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1801 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 1860

1801 cgagtgcgac atccatagag tcaagccaca tccagcaagc gaggttcgac ccgacacacg 1860

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1861 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 1920

1861 tgcttggggg gcaagtcggg ctggcgacgc ggaataggcc attgatagca gaactcaggt 1920

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1921 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 1980

1921 tgggccattc tgtgctgaat agcggtgacc gtcgtcggtg accattgtcc taatcgtctc 1980

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1981 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 2040

1981 gctccataca tccgccacga tgtctcaaga acttcaccac cggattgatg ccgatgtgat 2040

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2041 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 2100

2041 cttcttgtca taaaccatag acgcgagacg acttcggtca atggaagcct ttttctcaac 2100

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2101 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 2160

2101 catcgagaac taggccgttt gtttggtggc gaccatcgcc accaaaaaaa caaacgttcg 2160

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2161 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 2220

2161 tcgtctaatg cgcgtctttt tttcctagag ttcttctagg aaactagaaa agatgcccca 2220

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2221 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 2280

2221 gactgcgagt caccttgctt ttgagtgcaa ttccctaaaa ccagtactct aatagttttt 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 2340

2281 cctagaagtg gatctaggaa aatttaattt ttacttcaaa atttagttag atttcatata 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga 2400

2341 tactcatttg aaccagactg tcaatggtta cgaattagtc actccgtgga tagagtcgct 2400

.......... .......... .......... ....... ORF 2 Ampicillin .......

.......... .......... .......... .......... .......... ..........

2401 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac 2460

2401 agacagataa agcaagtagg tatcaacgga ctgaggggca gcacatctat tgatgctatg 2460

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg 2520

2461 ccctcccgaa tggtagaccg gggtcacgac gttactatgg cgctctgggt gcgagtggcc 2520

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg 2580

2521 gaggtctaaa tagtcgttat ttggtcggtc ggccttcccg gctcgcgtct tcaccaggac 2580

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... AseI(2608) .......... .......... ..........

2581 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt 2640

2581 gttgaaatag gcggaggtag gtcagataat taacaacggc ccttcgatct cattcatcaa 2640

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct 2700

2641 gcggtcaatt atcaaacgcg ttgcaacaac ggtaacgatg tccgtagcac cacagtgcga 2700

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat 2760

2701 gcagcaaacc ataccgaagt aagtcgaggc caagggttgc tagttccgct caatgtacta 2760

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta 2820

2761 gggggtacaa cacgtttttt cgccaatcga ggaagccagg aggctagcaa cagtcttcat 2820

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca 2880

2821 tcaaccggcg tcacaatagt gagtaccaat accgtcgtga cgtattaaga gaatgacagt 2880

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat 2940

2881 acggtaggca ttctacgaaa agacactgac cactcatgag ttggttcagt aagactctta 2940

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac 3000

2941 tcacatacgc cgctggctca acgagaacgg gccgcagtta tgccctatta tggcgcggtg 3000

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa 3060

3001 tatcgtcttg aaattttcac gagtagtaac cttttgcaag aagccccgct tttgagagtt 3060

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt 3120

3061 cctagaatgg cgacaactct aggtcaagct acattgggtg agcacgtggg ttgactagaa 3120

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg 3180

3121 gtcgtagaaa atgaaagtgg tcgcaaagac ccactcgttt ttgtccttcc gttttacggc 3180

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat 3240

3181 gttttttccc ttattcccgc tgtgccttta caacttatga gtatgagaag gaaaaagtta 3240

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt 3300

3241 taataacttc gtaaatagtc ccaataacag agtactcgcc tatgtataaa cttacataaa 3300

.......... .......... .......... AmpR_pro .......... ..........

.......... .......... .......... .......... .......... ..........

3301 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgcgc 3360

3301 tctttttatt tgtttatccc caaggcgcgt gtaaaggggc ttttcacggt ggactgcgcg 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac 3420

3361 ggacatcgcc gcgtaattcg cgccgcccac accaccaatg cgcgtcgcac tggcgatgtg 3420

.......... .......... .......... f1_origin .......... ..........

.......... .......... .......... .......... .......... ..........

3421 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg 3480

3421 aacggtcgcg ggatcgcggg cgaggaaagc gaaagaaggg aaggaaagag cggtgcaagc 3480

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt 3540

3481 ggccgaaagg ggcagttcga gatttagccc ccgagggaaa tcccaaggct aaatcacgaa 3540

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc 3600

3541 atgccgtgga gctggggttt tttgaactaa tcccactacc aagtgcatca cccggtagcg 3600

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct 3660

3601 ggactatctg ccaaaaagcg ggaaactgca acctcaggtg caagaaatta tcacctgaga 3660

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga 3720

3661 acaaggtttg accttgttgt gagttgggat agagccagat aagaaaacta aatattccct 3720

f1_origin .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga 3780

3721 aaaacggcta aagccggata accaattttt tactcgacta aattgttttt aaattgcgct 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 attttaacaa aatattaacg cttacaattt gccattcgcc attcaggctg cgcaactgtt 3840

3781 taaaattgtt ttataattgc gaatgttaaa cggtaagcgg taagtccgac gcgttgacaa 3840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gcccaagcta ccatgataag 3900

3841 cccttcccgc tagccacgcc cggagaagcg ataatgcggt cgggttcgat ggtactattc 3900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... NotI(3934) .......... ..........

3901 taagtaatat taaggtacgg gaggtacttg gagcggccgc aataaaatat ctttattttc 3960

3901 attcattata attccatgcc ctccatgaac ctcgccggcg ttattttata gaaataaaag 3960

.......... .......... .......... . synth_PA_term TB up_PA_term .

.......... .......... .......... .......... .......... ..........

3961 attacatctg tgtgttggtt ttttgtgtga atcgatagta ctaacatacg ctctccatca 4020

3961 taatgtagac acacaaccaa aaaacacact tagctatcat gattgtatgc gagaggtagt 4020

...... synth_PA_term TB up_PA_term ...... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 aaacaaaacg aaacaaaaca aactagcaaa ataggctgtc cccagtgcaa gtgcaggtgc 4080

4021 tttgttttgc tttgttttgt ttgatcgttt tatccgacag gggtcacgtt cacgtccacg 4080

.......... ........ TB up_PA_term RVpri3_pri ........ ..........

.......... .......... .......... .......... .......... ..........

4081 cagaacattt ctctatcgat a......... .......... .......... .......... 4101

4081 gtcttgtaaa gagatagcta t......... .......... .......... .......... 4101

TB up_PA_term ...... .......... .......... .......... ..........