Sequence of psiCHECK-1

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Sequence of psiCHECK-1

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Sequence of psiCHECK-1 : 3560 BP; 892 A; 892 C; 912 G; 864 T; 0 other;

BglII(1) .......... .......... .......... .......... ..........

1 agatctgcgc agcaccatgg cctgaaataa cctctgaaag aggaacttgg ttaggtacct 60

1 tctagacgcg tcgtggtacc ggactttatt ggagactttc tccttgaacc aatccatgga 60

.......... .......... .......... .......... .......... ..........

.......... PvuII(80) .......... .......... .......... ..........

61 tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag 120

61 agactccgcc tttcttggtc gacaccttac acacagtcaa tcccacacct ttcaggggtc 120

.......... .......... ......... pBABE_3_pri SV40_enh SV40_pro

.......... .......... .......... .......... .......... ..........

121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg 180

121 cgaggggtcg tccgtcttca tacgtttcgt acgtagagtt aatcagtcgt tggtccacac 180

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

181 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag 240

181 ctttcagggg tccgaggggt cgtccgtctt catacgtttc gtacgtagag ttaatcagtc 240

.......... ...... SV40_enh SV40_pro ...... .......... SV40_pro

.......... .......... .......... .......... .......... ..........

241 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc 300

241 gttggtatca gggcggggat tgaggcgggt agggcgggga ttgaggcggg tcaaggcggg 300

.......... .. SV40_enh SV40_pro SV40_pro . SV40_origin .........

.......... .......... .......... .......... .......... ..........

301 attctccgcc ccatggctga ctaatttttt tta tttatgc agaggccgag gccgcctcgg 360

301 taagaggcgg ggtaccgact gattaaaaaa aataaatacg tctccggctc cggcggagcc 360

.......... SV40_pro SV40_pro SV40_origin SV40pro_F ..........

.......... .......... .......... .......... StuI(403) ..........

361 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa 420

361 ggagactcga taaggtcttc atcactcctc cgaaaaaacc tccggatccg aaaacgtttt 420

SV40_pro .......... SV40_pro .......... .......... ..........

.......... .......... .......... .......... PstI(462) ..........

421 agcttgattc ttctgacaca acagtctcga acttaagctg cagaagttgg tcgtgaggca 480

421 tcgaactaag aagactgtgt tgtcagagct tgaattcgac gtcttcaacc agcactccgt 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 ctgggcaggt aagtatcaag gttacaagac aggtttaagg agaccaatag aaactgggct 540

481 gacccgtcca ttcatagttc caatgttctg tccaaattcc tctggttatc tttgacccga 540

.......... .......... .......... chim_int .......... ..........

.......... .......... .......... .......... .......... ..........

541 tgtcgagaca gagaagactc ttgcgtttct gataggcacc tattggtctt actgacatcc 600

541 acagctctgt ctcttctgag aacgcaaaga ctatccgtgg ataaccagaa tgactgtagg 600

.......... .......... chim_int .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 actttgcctt tctctccaca ggtgtccact cccagttcaa ttacagctct taaggctaga 660

601 tgaaacggaa agagaggtgt ccacaggtga gggtcaagtt aatgtcgaga attccgatct 660

.......... chim_int .......... .......... .......... ..........

.......... .......... NheI(684) .......... .......... ..........

661 gtacttaata cgactcacta taggctagcc accatggctt ccaaggtgta cgaccccgag 720

661 catgaattat gctgagtgat atccgatcgg tggtaccgaa ggttccacat gctggggctc 720

.......... T7 .......... .......... ORF1 ..........

.......... .......... .......... .......... .......... ..........

721 caacgcaaac gcatgatcac tgggcctcag tggtgggctc gctgcaagca aatgaacgtg 780

721 gttgcgtttg cgtactagtg acccggagtc accacccgag cgacgttcgt ttacttgcac 780

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 ctggactcct tcatcaacta ctatgattcc gagaagcacg ccgagaacgc cgtgattttt 840

781 gacctgagga agtagttgat gatactaagg ctcttcgtgc ggctcttgcg gcactaaaaa 840

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ctgcatggta acgctgcctc cagctacctg tggaggcacg tcgtgcctca catcgagccc 900

841 gacgtaccat tgcgacggag gtcgatggac acctccgtgc agcacggagt gtagctcggg 900

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 gtggctagat gcatcatccc tgatctgatc ggaatgggta agtccggcaa gagcgggaat 960

901 caccgatcta cgtagtaggg actagactag ccttacccat tcaggccgtt ctcgccctta 960

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 ggctcatatc gcctcctgga tcactacaag tacctcaccg cttggttcga gctgctgaac 1020

961 ccgagtatag cggaggacct agtgatgttc atggagtggc gaaccaagct cgacgacttg 1020

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 cttccaaaga aaatcatctt tgtgggccac gactgggggg cttgtctggc ctttcactac 1080

1021 gaaggtttct tttagtagaa acacccggtg ctgacccccc gaacagaccg gaaagtgatg 1080

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 tcctacgagc accaagacaa gatcaaggcc atcgtccatg ctgagagtgt cgtggacgtg 1140

1081 aggatgctcg tggttctgtt ctagttccgg tagcaggtac gactctcaca gcacctgcac 1140

.......... .......... ORF1 .......... .......... ..........

.......... .......... ......... EcoRV(1179) ......... ..........

1141 atcgagtcct gggacgagtg gcctgacatc gaggaggata tcgccctgat caagagcgaa 1200

1141 tagctcagga ccctgctcac cggactgtag ctcctcctat agcgggacta gttctcgctt 1200

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 gagggcgaga aaatggtgct tgagaataac ttcttcgtcg agaccatgct cccaagcaag 1260

1201 ctcccgctct tttaccacga actcttattg aagaagcagc tctggtacga gggttcgttc 1260

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 atcatgcgga aactggagcc tgaggagttc gctgcctacc tggagccatt caaggagaag 1320

1261 tagtacgcct ttgacctcgg actcctcaag cgacggatgg acctcggtaa gttcctcttc 1320

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... NruI(1355) .......... ..........

1321 ggcgaggtta gacggcctac cctctcctgg cctcgcgaga tccctctcgt taagggaggc 1380

1321 ccgctccaat ctgccggatg ggagaggacc ggagcgctct agggagagca attccctccg 1380

.......... .......... ORF1 .......... .......... ..........

......... AatII(1391) ......... .......... .......... ..........

1381 aagcccgacg tcgtccagat tgtccgcaac tacaacgcct accttcgggc cagcgacgat 1440

1381 ttcgggctgc agcaggtcta acaggcgttg atgttgcgga tggaagcccg gtcgctgcta 1440

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 ctgcctaaga tgttcatcga gtccgaccct gggttctttt ccaacgctat tgtcgaggga 1500

1441 gacggattct acaagtagct caggctggga cccaagaaaa ggttgcgata acagctccct 1500

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 gctaagaagt tccctaacac cgagttcgtg aaggtgaagg gcctccactt cagccaggag 1560

1501 cgattcttca agggattgtg gctcaagcac ttccacttcc cggaggtgaa gtcggtcctc 1560

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 gacgctccag atgaaatggg taagtacatc aagagcttcg tggagcgcgt gctgaagaac 1620

1561 ctgcgaggtc tactttaccc attcatgtag ttctcgaagc acctcgcgca cgacttcttg 1620

.......... .......... ORF1 .......... .......... ..........

.......... .. XhoI SmaI(1651),EcoRI(1654) NotI(1674),EagI(1674)

1621 gagcagtaat tctaggcgat cgctcgagcc cgggaattcg tttaaaccta gagcggccgc 1680

1621 ctcgtcatta agatccgcta gcgagctcgg gcccttaagc aaatttggat ctcgccggcg 1680

ORF1 .......... .......... .......... .......... ..........

.......... .......... .......... .......... ......... BamHI(1738)

1681 tggccgcaat aaaatatctt tattttcatt acatctgtgt gttggttttt tgtgtgagga 1740

1681 accggcgtta ttttatagaa ataaaagtaa tgtagacaca caaccaaaaa acacactcct 1740

.......... .......... ........ synth_PA_term ........ ..........

.......... .......... .......... .......... .......... ..........

1741 tccaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat 1800

1741 aggtccaccg tgaaaagccc ctttacacgc gccttgggga taaacaaata aaaagattta 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg 1860

1801 tgtaagttta tacataggcg agtactctgt tattgggact atttacgaag ttattataac 1860

.......... AmpR_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc 1920

1861 tttttccttc tcatactcat aagttgtaaa ggcacagcgg gaataaggga aaaaacgccg 1920

.......... .......... ....... ORF2 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

1921 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga 1980

1921 taaaacggaa ggacaaaaac gagtgggtct ttgcgaccac tttcattttc tacgacttct 1980

.......... ....... ORF2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga 2040

1981 agtcaaccca cgtgctcacc caatgtagct tgacctagag ttgtcgccat tctaggaact 2040

.......... ....... ORF2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg 2100

2041 ctcaaaagcg gggcttcttg caaaaggtta ctactcgtga aaatttcaag acgatacacc 2100

.......... ....... ORF2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc 2160

2101 gcgccataat agggcataac tgcggcccgt tctcgttgag ccagcggcgt atgtgataag 2160

.......... ....... ORF2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac 2220

2161 agtcttactg aaccaactca tgagtggtca gtgtcttttc gtagaatgcc taccgtactg 2220

.......... ....... ORF2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact 2280

2221 tcattctctt aatacgtcac gacggtattg gtactcacta ttgtgacgcc ggttgaatga 2280

.......... ....... ORF2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca 2340

2281 agactgttgc tagcctcctg gcttcctcga ttggcgaaaa aacgtgttgt accccctagt 2340

.......... ....... ORF2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg 2400

2341 acattgagcg gaactagcaa cccttggcct cgacttactt cggtatggtt tgctgctcgc 2400

.......... ....... ORF2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact 2460

2401 actgtggtgc tacggacatc gttaccgttg ttgcaacgcg tttgataatt gaccgcttga 2460

.......... ....... ORF2 Ampicillin ....... .......... ..........

.......... .......... AseI(2486) .......... .......... ..........

2461 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg 2520

2461 tgaatgagat cgaagggccg ttgttaatta tctgacctac ctccgcctat ttcaacgtcc 2520

.......... ....... ORF2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg 2580

2521 tggtgaagac gcgagccggg aaggccgacc gaccaaataa cgactattta gacctcggcc 2580

.......... ....... ORF2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat 2640

2581 actcgcaccc agagcgccat agtaacgtcg tgaccccggt ctaccattcg ggagggcata 2640

.......... ....... ORF2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc 2700

2641 gcatcaatag atgtgctgcc cctcagtccg ttgataccta cttgctttat ctgtctagcg 2700

.......... ....... ORF2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat 2760

2701 actctatcca cggagtgact aattcgtaac cattgacagt ctggttcaaa tgagtatata 2760

....... ORF2 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt 2820

2761 tgaaatctaa ctaaattttg aagtaaaaat taaattttcc tagatccact tctaggaaaa 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc 2880

2821 actattagag tactggtttt agggaattgc actcaaaagc aaggtgactc gcagtctggg 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt 2940

2881 gcatcttttc tagtttccta gaagaactct aggaaaaaaa gacgcgcatt agacgacgaa 2940

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

2941 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac 3000

2941 cgtttgtttt tttggtggcg atggtcgcca ccaaacaaac ggcctagttc tcgatggttg 3000

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt 3060

3001 agaaaaaggc ttccattgac cgaagtcgtc tcgcgtctat ggtttatgac aagaagatca 3060

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3061 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct 3120

3061 catcggcatc aatccggtgg tgaagttctt gagacatcgt ggcggatgta tggagcgaga 3120

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3121 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga 3180

3121 cgattaggac aatggtcacc gacgacggtc accgctattc agcacagaat ggcccaacct 3180

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3181 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac 3240

3181 gagttctgct atcaatggcc tattccgcgt cgccagcccg acttgccccc caagcacgtg 3240

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3241 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg 3300

3241 tgtcgggtcg aacctcgctt gctggatgtg gcttgactct atggatgtcg cactcgatac 3300

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3301 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt 3360

3301 tctttcgcgg tgcgaagggc ttccctcttt ccgcctgtcc ataggccatt cgccgtccca 3360

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3361 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc 3420

3361 gccttgtcct ctcgcgtgct ccctcgaagg tccccctttg cggaccatag aaatatcagg 3420

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3421 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg 3480

3421 acagcccaaa gcggtggaga ctgaactcgc agctaaaaac actacgagca gtccccccgc 3480

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3481 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc 3540

3481 ctcggatacc tttttgcggt cgttgcgccg gaaaaatgcc aaggaccgga aaacgaccgg 3540

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 ttttgctcac atggctcgac .......... .......... .......... .......... 3560

3541 aaaacgagtg taccgagctg .......... .......... .......... .......... 3560

.......... .......... .......... .......... .......... ..........