Sequence of psiCHECK-2

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Sequence of psiCHECK-2

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Sequence of psiCHECK-2 : 6273 BP; 1460 A; 1668 C; 1725 G; 1420 T; 0 other;

BglII(1) .......... .......... .......... .......... KpnI(58)

1 agatctgcgc agcaccatgg cctgaaataa cctctgaaag aggaacttgg ttaggtacct 60

1 tctagacgcg tcgtggtacc ggactttatt ggagactttc tccttgaacc aatccatgga 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag 120

61 agactccgcc tttcttggtc gacaccttac acacagtcaa tcccacacct ttcaggggtc 120

.......... .......... ......... pBABE_3_pri SV40_enh SV40_pro

.......... .......... .......... .......... .......... ..........

121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg 180

121 cgaggggtcg tccgtcttca tacgtttcgt acgtagagtt aatcagtcgt tggtccacac 180

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

181 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag 240

181 ctttcagggg tccgaggggt cgtccgtctt catacgtttc gtacgtagag ttaatcagtc 240

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

241 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc 300

241 gttggtatca gggcggggat tgaggcgggt agggcgggga ttgaggcggg tcaaggcggg 300

.......... .. SV40_enh SV40_pro SV40_ori SV40_pro .. ..........

.......... .......... .......... .......... .......... ..........

301 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg 360

301 taagaggcgg ggtaccgact gattaaaaaa aataaatacg tctccggctc cggcggagcc 360

.......... .. SV40_pro SV40_ori SV40_pro .. SV40pro_F ..........

.......... .......... .......... .......... StuI(403) ..........

361 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa 420

361 ggagactcga taaggtcttc atcactcctc cgaaaaaacc tccggatccg aaaacgtttt 420

SV40_pro .......... SV40_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 agcttgattc ttctgacaca acagtctcga acttaagctg cagaagttgg tcgtgaggca 480

421 tcgaactaag aagactgtgt tgtcagagct tgaattcgac gtcttcaacc agcactccgt 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 ctgggcaggt aagtatcaag gttacaagac aggtttaagg agaccaatag aaactgggct 540

481 gacccgtcca ttcatagttc caatgttctg tccaaattcc tctggttatc tttgacccga 540

.......... .......... .......... chim_int .......... ..........

.......... .......... .......... .......... .......... ..........

541 tgtcgagaca gagaagactc ttgcgtttct gataggcacc tattggtctt actgacatcc 600

541 acagctctgt ctcttctgag aacgcaaaga ctatccgtgg ataaccagaa tgactgtagg 600

.......... .......... chim_int .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 actttgcctt tctctccaca ggtgtccact cccagttcaa ttacagctct taaggctaga 660

601 tgaaacggaa agagaggtgt ccacaggtga gggtcaagtt aatgtcgaga attccgatct 660

.......... chim_int .......... .......... .......... ..........

.......... .......... NheI(684) .......... .......... ..........

661 gtacttaata cgactcacta taggctagcc accatggctt ccaaggtgta cgaccccgag 720

661 catgaattat gctgagtgat atccgatcgg tggtaccgaa ggttccacat gctggggctc 720

.......... T7 .......... .......... ORF1 ..........

.......... .......... .......... .......... .......... ..........

721 caacgcaaac gcatgatcac tgggcctcag tggtgggctc gctgcaagca aatgaacgtg 780

721 gttgcgtttg cgtactagtg acccggagtc accacccgag cgacgttcgt ttacttgcac 780

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 ctggactcct tcatcaacta ctatgattcc gagaagcacg ccgagaacgc cgtgattttt 840

781 gacctgagga agtagttgat gatactaagg ctcttcgtgc ggctcttgcg gcactaaaaa 840

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ctgcatggta acgctgcctc cagctacctg tggaggcacg tcgtgcctca catcgagccc 900

841 gacgtaccat tgcgacggag gtcgatggac acctccgtgc agcacggagt gtagctcggg 900

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 gtggctagat gcatcatccc tgatctgatc ggaatgggta agtccggcaa gagcgggaat 960

901 caccgatcta cgtagtaggg actagactag ccttacccat tcaggccgtt ctcgccctta 960

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 ggctcatatc gcctcctgga tcactacaag tacctcaccg cttggttcga gctgctgaac 1020

961 ccgagtatag cggaggacct agtgatgttc atggagtggc gaaccaagct cgacgacttg 1020

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 cttccaaaga aaatcatctt tgtgggccac gactgggggg cttgtctggc ctttcactac 1080

1021 gaaggtttct tttagtagaa acacccggtg ctgacccccc gaacagaccg gaaagtgatg 1080

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 tcctacgagc accaagacaa gatcaaggcc atcgtccatg ctgagagtgt cgtggacgtg 1140

1081 aggatgctcg tggttctgtt ctagttccgg tagcaggtac gactctcaca gcacctgcac 1140

.......... .......... ORF1 .......... .......... ..........

.......... .......... ......... EcoRV(1179) ......... ..........

1141 atcgagtcct gggacgagtg gcctgacatc gaggaggata tcgccctgat caagagcgaa 1200

1141 tagctcagga ccctgctcac cggactgtag ctcctcctat agcgggacta gttctcgctt 1200

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 gagggcgaga aaatggtgct tgagaataac ttcttcgtcg agaccatgct cccaagcaag 1260

1201 ctcccgctct tttaccacga actcttattg aagaagcagc tctggtacga gggttcgttc 1260

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 atcatgcgga aactggagcc tgaggagttc gctgcctacc tggagccatt caaggagaag 1320

1261 tagtacgcct ttgacctcgg actcctcaag cgacggatgg acctcggtaa gttcctcttc 1320

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... NruI(1355) .......... ..........

1321 ggcgaggtta gacggcctac cctctcctgg cctcgcgaga tccctctcgt taagggaggc 1380

1321 ccgctccaat ctgccggatg ggagaggacc ggagcgctct agggagagca attccctccg 1380

.......... .......... ORF1 .......... .......... ..........

......... AatII(1391) ......... .......... .......... ..........

1381 aagcccgacg tcgtccagat tgtccgcaac tacaacgcct accttcgggc cagcgacgat 1440

1381 ttcgggctgc agcaggtcta acaggcgttg atgttgcgga tggaagcccg gtcgctgcta 1440

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 ctgcctaaga tgttcatcga gtccgaccct gggttctttt ccaacgctat tgtcgaggga 1500

1441 gacggattct acaagtagct caggctggga cccaagaaaa ggttgcgata acagctccct 1500

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 gctaagaagt tccctaacac cgagttcgtg aaggtgaagg gcctccactt cagccaggag 1560

1501 cgattcttca agggattgtg gctcaagcac ttccacttcc cggaggtgaa gtcggtcctc 1560

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 gacgctccag atgaaatggg taagtacatc aagagcttcg tggagcgcgt gctgaagaac 1620

1561 ctgcgaggtc tactttaccc attcatgtag ttctcgaagc acctcgcgca cgacttcttg 1620

.......... .......... ORF1 .......... .......... ..........

.......... .......... XhoI(1643) .......... .......... NotI(1674)

1621 gagcagtaat tctaggcgat cgctcgagcc cgggaattcg tttaaaccta gagcggccgc 1680

1621 ctcgtcatta agatccgcta gcgagctcgg gcccttaagc aaatttggat ctcgccggcg 1680

ORF1 .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 tggccgcaat aaaatatctt tattttcatt acatctgtgt gttggttttt tgtgtgagga 1740

1681 accggcgtta ttttatagaa ataaaagtaa tgtagacaca caaccaaaaa acacactcct 1740

.......... .......... ........ synth_PA_term ........ ..........

.......... .......... .......... .......... .......... ..........

1741 tctaaatgag tcttcggacc tcgcgggggc cgcttaagcg gtggttaggg tttgtctgac 1800

1741 agatttactc agaagcctgg agcgcccccg gcgaattcgc caccaatccc aaacagactg 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 gcggggggag ggggaaggaa cgaaacactc tcattcggag gcggctcggg gtttggtctt 1860

1801 cgcccccctc ccccttcctt gctttgtgag agtaagcctc cgccgagccc caaaccagaa 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 ggtggccacg ggcacgcaga agagcgccgc gatcctctta agcacccccc cgccctccgt 1920

1861 ccaccggtgc ccgtgcgtct tctcgcggcg ctaggagaat tcgtgggggg gcgggaggca 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ggaggcgggg gtttggtcgg cgggtggtaa ctggcgggcc gctgactcgg gcgggtcgcg 1980

1921 cctccgcccc caaaccagcc gcccaccatt gaccgcccgg cgactgagcc cgcccagcgc 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... ......... SacII(2036)

1981 cgccccagag tgtgaccttt tcggtctgct cgcagacccc cgggcggcgc cgccgcggcg 2040

1981 gcggggtctc acactggaaa agccagacga gcgtctgggg gcccgccgcg gcggcgccgc 2040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 gcgacgggct cgctgggtcc taggctccat ggggaccgta tacgtggaca ggctctggag 2100

2041 cgctgcccga gcgacccagg atccgaggta cccctggcat atgcacctgt ccgagacctc 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 catccgcacg actgcggtga tattaccgga gaccttctgc gggacgagcc gggtcacgcg 2160

2101 gtaggcgtgc tgacgccact ataatggcct ctggaagacg ccctgctcgg cccagtgcgc 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 gctgacgcgg agcgtccgtt gggcgacaaa caccaggacg gggcacaggt acactatctt 2220

2161 cgactgcgcc tcgcaggcaa cccgctgttt gtggtcctgc cccgtgtcca tgtgatagaa 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 gtcacccgga ggcgcgaggg actgcaggag cttcagggag tggcgcagct gcttcatccc 2280

2221 cagtgggcct ccgcgctccc tgacgtcctc gaagtccctc accgcgtcga cgaagtaggg 2280

.......... .......... .......... .......... .......... TATA_pro

.......... .......... .......... .......... .......... ..........

2281 cgtggcccgt tgctcgcgtt tgctggcggt gtccccggaa gaaatatatt tgcatgtctt 2340

2281 gcaccgggca acgagcgcaa acgaccgcca caggggcctt ctttatataa acgtacagaa 2340

.......... ....... TATA_pro TK_pro ....... .......... ..........

.......... .......... .......... ......... BstBI(2390) .........

2341 tagttctatg atgacacaaa ccccgcccag cgtcttgtca ttggcgaatt cgaacacgca 2400

2341 atcaagatac tactgtgttt ggggcgggtc gcagaacagt aaccgcttaa gcttgtgcgt 2400

.......... ....... TATA_pro TK_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 gatgcagtcg gggcggcgcg gtcccaggtc cacttcgcat attaaggtga cgcgtgtggc 2460

2401 ctacgtcagc cccgccgcgc cagggtccag gtgaagcgta taattccact gcgcacaccg 2460

TATA_pro .......... TK_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ctcgaacacc gagcgaccct gcagcgaccc gcttaaaagc ttggcattcc ggtactgttg 2520

2461 gagcttgtgg ctcgctggga cgtcgctggg cgaattttcg aaccgtaagg ccatgacaac 2520

.......... TK_pro .......... .......... ORF3 ..........

.......... .......... .......... .......... ApaI(2562) ..........

2521 gtaaagccac catggccgat gctaagaaca ttaagaaggg ccctgctccc ttctaccctc 2580

2521 catttcggtg gtaccggcta cgattcttgt aattcttccc gggacgaggg aagatgggag 2580

.......... .......... ORF3 ORF3 .......... ..........

.......... .......... .......... .......... .......... ..........

2581 tggaggatgg caccgctggc gagcagctgc acaaggccat gaagaggtat gccctggtgc 2640

2581 acctcctacc gtggcgaccg ctcgtcgacg tgttccggta cttctccata cgggaccacg 2640

.......... .......... ORF3 ORF3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 ctggcaccat tgccttcacc gatgcccaca ttgaggtgga catcacctat gccgagtact 2700

2641 gaccgtggta acggaagtgg ctacgggtgt aactccacct gtagtggata cggctcatga 2700

.......... .......... ORF3 ORF3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 tcgagatgtc tgtgcgcctg gccgaggcca tgaagaggta cggcctgaac accaaccacc 2760

2701 agctctacag acacgcggac cggctccggt acttctccat gccggacttg tggttggtgg 2760

.......... .......... ORF3 ORF3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 gcatcgtggt gtgctctgag aactctctgc agttcttcat gccagtgctg ggcgccctgt 2820

2761 cgtagcacca cacgagactc ttgagagacg tcaagaagta cggtcacgac ccgcgggaca 2820

.......... .......... ORF3 ORF3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 tcatcggagt ggccgtggcc cctgctaacg acatttacaa cgagcgcgag ctgctgaaca 2880

2821 agtagcctca ccggcaccgg ggacgattgc tgtaaatgtt gctcgcgctc gacgacttgt 2880

.......... .......... ORF3 ORF3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gcatgggcat ttctcagcct accgtggtgt tcgtgtctaa gaagggcctg cagaagatcc 2940

2881 cgtacccgta aagagtcgga tggcaccaca agcacagatt cttcccggac gtcttctagg 2940

.......... .......... ORF3 ORF3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 tgaacgtgca gaagaagctg cctatcatcc agaagatcat catcatggac tctaagaccg 3000

2941 acttgcacgt cttcttcgac ggatagtagg tcttctagta gtagtacctg agattctggc 3000

.......... .......... ORF3 ORF3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 actaccaggg cttccagagc atgtacacat tcgtgacatc tcatctgcct cctggcttca 3060

3001 tgatggtccc gaaggtctcg tacatgtgta agcactgtag agtagacgga ggaccgaagt 3060

.......... .......... ORF3 ORF3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 acgagtacga cttcgtgcca gagtctttcg acagggacaa aaccattgcc ctgatcatga 3120

3061 tgctcatgct gaagcacggt ctcagaaagc tgtccctgtt ttggtaacgg gactagtact 3120

.......... .......... ORF3 ORF3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 acagctctgg gtctaccggc ctgcctaagg gcgtggccct gcctcatcgc accgcctgtg 3180

3121 tgtcgagacc cagatggccg gacggattcc cgcaccggga cggagtagcg tggcggacac 3180

.......... .......... ORF3 ORF3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 tgcgcttctc tcacgcccgc gaccctattt tcggcaacca gatcatcccc gacaccgcta 3240

3181 acgcgaagag agtgcgggcg ctgggataaa agccgttggt ctagtagggg ctgtggcgat 3240

.......... .......... ORF3 ORF3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 ttctgagcgt ggtgccattc caccacggct tcggcatgtt caccaccctg ggctacctga 3300

3241 aagactcgca ccacggtaag gtggtgccga agccgtacaa gtggtgggac ccgatggact 3300

.......... .......... ORF3 ORF3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 tttgcggctt tcgggtggtg ctgatgtacc gcttcgagga ggagctgttc ctgcgcagcc 3360

3301 aaacgccgaa agcccaccac gactacatgg cgaagctcct cctcgacaag gacgcgtcgg 3360

.......... .......... ORF3 ORF3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 tgcaagacta caaaattcag tctgccctgc tggtgccaac cctgttcagc ttcttcgcta 3420

3361 acgttctgat gttttaagtc agacgggacg accacggttg ggacaagtcg aagaagcgat 3420

.......... .......... ORF3 ORF3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 agagcaccct gatcgacaag tacgacctgt ctaacctgca cgagattgcc tctggcggcg 3480

3421 tctcgtggga ctagctgttc atgctggaca gattggacgt gctctaacgg agaccgccgc 3480

.......... .......... ORF3 ORF3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 ccccactgtc taaggaggtg ggcgaagccg tggccaagcg ctttcatctg ccaggcatcc 3540

3481 ggggtgacag attcctccac ccgcttcggc accggttcgc gaaagtagac ggtccgtagg 3540

.......... .......... ORF3 ORF3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 gccagggcta cggcctgacc gagacaacca gcgccattct gattacccca gagggcgacg 3600

3541 cggtcccgat gccggactgg ctctgttggt cgcggtaaga ctaatggggt ctcccgctgc 3600

.......... .......... ORF3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 acaagcctgg cgccgtgggc aaggtggtgc cattcttcga ggccaaggtg gtggacctgg 3660

3601 tgttcggacc gcggcacccg ttccaccacg gtaagaagct ccggttccac cacctggacc 3660

.......... .......... ORF3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 acaccggcaa gaccctggga gtgaaccagc gcggcgagct gtgtgtgcgc ggccctatga 3720

3661 tgtggccgtt ctgggaccct cacttggtcg cgccgctcga cacacacgcg ccgggatact 3720

.......... .......... ORF3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ttatgtccgg ctacgtgaat aaccctgagg ccacaaacgc cctgatcgac aaggacggct 3780

3721 aatacaggcc gatgcactta ttgggactcc ggtgtttgcg ggactagctg ttcctgccga 3780

.......... .......... ORF3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 ggctgcactc tggcgacatt gcctactggg acgaggacga gcacttcttc atcgtggacc 3840

3781 ccgacgtgag accgctgtaa cggatgaccc tgctcctgct cgtgaagaag tagcacctgg 3840

.......... .......... ORF3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 gcctgaagtc tctgatcaag tacaagggct accaggtggc cccagccgag ctggagtcta 3900

3841 cggacttcag agactagttc atgttcccga tggtccaccg gggtcggctc gacctcagat 3900

.......... .......... ORF3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 tcctgctgca gcaccctaac attttcgacg ccggagtggc cggcctgccc gacgacgatg 3960

3901 aggacgacgt cgtgggattg taaaagctgc ggcctcaccg gccggacggg ctgctgctac 3960

.......... .......... ORF3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 ccggcgagct gcctgccgcc gtcgtcgtgc tggaacacgg caagaccatg accgagaagg 4020

3961 ggccgctcga cggacggcgg cagcagcacg accttgtgcc gttctggtac tggctcttcc 4020

.......... .......... ORF3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 agatcgtgga ctatgtggcc agccaggtga caaccgccaa gaagctgcgc ggcggagtgg 4080

4021 tctagcacct gatacaccgg tcggtccact gttggcggtt cttcgacgcg ccgcctcacc 4080

.......... .......... ORF3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 tgttcgtgga cgaggtgccc aagggcctga ccggcaagct ggacgcccgc aagatccgcg 4140

4081 acaagcacct gctccacggg ttcccggact ggccgttcga cctgcgggcg ttctaggcgc 4140

.......... .......... ORF3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 agatcctgat caaggctaag aaaggcggca agatcgccgt gtaataattc tagagtcggg 4200

4141 tctaggacta gttccgattc tttccgccgt tctagcggca cattattaag atctcagccc 4200

.......... .......... ORF3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 gcggccggcc gcttcgagca gacatgataa gatacattga tgagtttgga caaaccacaa 4260

4201 cgccggccgg cgaagctcgt ctgtactatt ctatgtaact actcaaacct gtttggtgtt 4260

.......... .......... .......... ...... SV40_PA_term EBV_r ......

.......... .......... .......... .......... .......... ..........

4261 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg 4320

4261 gatcttacgt cacttttttt acgaaataaa cactttaaac actacgataa cgaaataaac 4320

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc 4380

4321 attggtaata ttcgacgtta tttgttcaat tgttgttgtt aacgtaagta aaatacaaag 4380

......... SV40_PA_term ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta 4440

4381 tccaagtccc cctccacacc ctccaaaaaa tttcgttcat tttggagatg tttacaccat 4440

.......... .......... .......... .......... .......... ..........

ClaI(4444) BamHI(4451) ........ .......... .......... ..........

4441 aaatcgataa ggatccaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt 4500

4441 tttagctatt cctaggtcca ccgtgaaaag cccctttaca cgcgccttgg ggataaacaa 4500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc 4560

4501 ataaaaagat ttatgtaagt ttatacatag gcgagtactc tgttattggg actatttacg 4560

.......... .......... .......... AmpR_pro .......... ..........

.......... .......... .......... .......... .......... ..........

4561 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc 4620

4561 aagttattat aactttttcc ttctcatact cataagttgt aaaggcacag cgggaataag 4620

.......... .......... .......... ....... ORF3 Ampicillin .......

.......... .......... .......... .......... .......... ..........

4621 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa 4680

4621 ggaaaaaacg ccgtaaaacg gaaggacaaa aacgagtggg tctttgcgac cactttcatt 4680

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg 4740

4681 ttctacgact tctagtcaac ccacgtgctc acccaatgta gcttgaccta gagttgtcgc 4740

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag 4800

4741 cattctagga actctcaaaa gcggggcttc ttgcaaaagg ttactactcg tgaaaatttc 4800

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc 4860

4801 aagacgatac accgcgccat aatagggcat aactgcggcc cgttctcgtt gagccagcgg 4860

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta 4920

4861 cgtatgtgat aagagtctta ctgaaccaac tcatgagtgg tcagtgtctt ttcgtagaat 4920

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg 4980

4921 gcctaccgta ctgtcattct cttaatacgt cacgacggta ttggtactca ctattgtgac 4980

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca 5040

4981 gccggttgaa tgaagactgt tgctagcctc ctggcttcct cgattggcga aaaaacgtgt 5040

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac 5100

5041 tgtaccccct agtacattga gcggaactag caacccttgg cctcgactta cttcggtatg 5100

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat 5160

5101 gtttgctgct cgcactgtgg tgctacggac atcgttaccg ttgttgcaac gcgtttgata 5160

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... AseI(5199) .......... ..........

5161 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg 5220

5161 attgaccgct tgatgaatga gatcgaaggg ccgttgttaa ttatctgacc tacctccgcc 5220

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata 5280

5221 tatttcaacg tcctggtgaa gacgcgagcc gggaaggccg accgaccaaa taacgactat 5280

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta 5340

5281 ttagacctcg gccactcgca cccagagcgc catagtaacg tcgtgacccc ggtctaccat 5340

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa 5400

5341 tcgggagggc atagcatcaa tagatgtgct gcccctcagt ccgttgatac ctacttgctt 5400

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag 5460

5401 tatctgtcta gcgactctat ccacggagtg actaattcgt aaccattgac agtctggttc 5460

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg 5520

5461 aaatgagtat atatgaaatc taactaaatt ttgaagtaaa aattaaattt tcctagatcc 5520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact 5580

5521 acttctagga aaaactatta gagtactggt tttagggaat tgcactcaaa agcaaggtga 5580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg 5640

5581 ctcgcagtct ggggcatctt ttctagtttc ctagaagaac tctaggaaaa aaagacgcgc 5640

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

5641 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc 5700

5641 attagacgac gaacgtttgt ttttttggtg gcgatggtcg ccaccaaaca aacggcctag 5700

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5701 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata 5760

5701 ttctcgatgg ttgagaaaaa ggcttccatt gaccgaagtc gtctcgcgtc tatggtttat 5760

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5761 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta 5820

5761 gacaagaaga tcacatcggc atcaatccgg tggtgaagtt cttgagacat cgtggcggat 5820

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5821 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc 5880

5821 gtatggagcg agacgattag gacaatggtc accgacgacg gtcaccgcta ttcagcacag 5880

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5881 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg 5940

5881 aatggcccaa cctgagttct gctatcaatg gcctattccg cgtcgccagc ccgacttgcc 5940

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5941 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac 6000

5941 ccccaagcac gtgtgtcggg tcgaacctcg cttgctggat gtggcttgac tctatggatg 6000

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6001 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg 6060

6001 tcgcactcga tactctttcg cggtgcgaag ggcttccctc tttccgcctg tccataggcc 6060

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6061 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt 6120

6061 attcgccgtc ccagccttgt cctctcgcgt gctccctcga aggtccccct ttgcggacca 6120

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6121 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct 6180

6121 tagaaatatc aggacagccc aaagcggtgg agactgaact cgcagctaaa aacactacga 6180

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6181 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg 6240

6181 gcagtccccc cgcctcggat acctttttgc ggtcgttgcg ccggaaaaat gccaaggacc 6240

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6241 ccttttgctg gccttttgct cacatggctc gac....... .......... .......... 6273

6241 ggaaaacgac cggaaaacga gtgtaccgag ctg....... .......... .......... 6273

.......... .......... .......... .......... .......... ..........