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Second Harmonic Generation Microscopy Imaging of Cornea after Femtosecond Laser Intrastromal Ablation
WANG Yan-yan1, HAN-Meng2, SUN Hui2, REN Qiu-shi1, JOSEF Bille2
(1.Laboratory of Visual Optics, Institute for Laser Medicine and Bio-Photonics, College of Life Science, Shanghai Jiao-Tong University, Shanghai 200030, China; 2.Heidelberg college Kirchhoff Institute for Physic）
Abstract:In this article Second Harmonic Generation (SHG) microscopy is utilized to investigate the structrual modification of cornea after laser treatment. With SHG microscopy which is widely used in biological researches, material sciences, a three-dimensional image stack of cornea can be abtained by optical sectioning noninvasively, the resolution is 500 nm.The imaging setup based on two photon micrscope. From the imaging results, the peformance of femtosecond laser in cornea ablation is evaluated, femtosecond laser can really ablate cornea precisely in several um range, the conclution of this experiment can support femtosecond laser to be applicated in clinics.
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