李步洪1, 2， 杨浦文3， 张镇西1， 谢树森2
（1.西安交通大学生命科学与技术学院 生物医学信息工程教育部重点实验室 生物医学工程研究所，中国陕西 西安710029；2.福建师范大学激光与光电子技术研究所，中国福建 福州 350007；3.南京军区福州总医院耳鼻喉科，中国福建 福州 3500025）
摘 要：利用激光诱导荧光方法实验测定了新型光敏剂血啉甲醚（Hematoporphyrin Monomethyl Ether, HMME）在鼻咽癌细胞株CNE中的时间分布和浓度变化规律。实验结果表明，鼻咽癌细胞株CNE的荧光光谱中的两个特征峰615nm和675nm证实了鼻咽癌细胞对HMME的选择性特异吸收。HMME在鼻咽癌细胞中的潴留浓度在混合培养后的140分钟达到最大值。当用于培养鼻咽癌细胞的HMME药物浓度低于32 (g/ml时，测量得到的相对荧光光谱强度与HMME浓度呈现出很好的线性关系，通过拟合方程可以间接确定在给定注射剂量条件下鼻咽癌细胞中所潴留HMME的浓度。结果可以为HMME在光动力学诊断与治疗中的临床应用提供理论依据和剂量参考。
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viiTime Dependence and Concentration of Cellular Uptake of Hematoporphyrin Monomethyl Ether in Nasopharyngeal Carcinoma Cells
LI Bu-hong 1,2, YANG Fu-wen 3, ZHANG Zhen-xi 1, XIE Shu-sen 2
(1.The Key Laboratory of Biomedical Information Engineering of Ministry of Education, and Institute of Biomedical Engineering, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, Shanxi, China；2. Institute of Laser and Optoelectronics Technology, Fujian Normal University, Fuzhou 350007,Fujian, China；3.Department of Otorhinolaryngology, Fuzhou General Hospital of the PLA , Fuzhou 350025 , Fujian, China）
Abstract：Time dependence and concentration of cellular selective uptake of new photosensitizer Hematoporphyrin monomethyl ether (HMME) in human nasopharyngeal carcinoma cell line CNE have been studied and determined by incubated CNE cells with HMME. Experimental results show that the peak emission fluorescence at the wavelength of 615 and 675 nm characterized the selective accumulation of HMME in CNE cells. The curve for time dependence of HMME in CNE cells indicate that the highest uptake peak appear in about 140 mins after incubation. When the concentration of HMME for incubated CNE cells under 32 (g/ml, the fluorescence intensity versus HMME concentration reveals an obvious linearity. These results can be used to improve the efficiency of photodynamic therapy and the accuracy of optical diagnosis for nasopharyngeal carcinoma with HMME.
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