刘天夫1, 陈碧芳1，2*
(1.中国计量学院信息工程系, 中国浙江 杭州310018；2.嘉兴学院医学院，中国浙江 嘉兴314001 )
摘 要：研究用于癌症诊断与治疗的光敏剂血卟啉（hematoporphyrin derivative，HPD）的超快光动力学过程，采用超短脉冲激光光谱技术和皮秒时间相关单光子计数系统，测量经血卟啉培养的活体癌细胞与正常细胞的荧光光谱、荧光寿命特性及荧光峰值强度随时间变化曲线，并测量单一细胞内部不同位置的荧光寿命特性，观测到：癌细胞样品在645nm处具有特有的光谱谱峰；癌细胞样品荧光寿命的快成分约150ps慢成分约1200ps，而正常细胞样品快成分约300ps慢成分约2500ps；癌细胞样品的荧光峰值强度经12小时衰减约10%，而正常细胞样品衰减约55%；在细胞内部荧光寿命300ps的快成分十分显著，且中心部位血卟啉浓度最高。癌细胞与正常细胞的荧光光谱、荧光寿命特性及荧光峰值强度随时间变化曲线相差十分明显，反映了癌细胞与正常细胞对血卟啉亲和特性有显著的差异，测量结果确认了荧光光谱技术诊断与治疗癌症的可行性，并对发展超短脉冲激光光谱技术早期诊断与治疗癌症具有重要的指导意义和临床应用价值。
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Distributive Feature of Soil Microorganism of Pinus Tabulaeformis Plantation Forestland in Loess Plateau
ZHANG She-qi1, WANG Guo-dong1, LIU Yun-peng1, LIU Jian-jun2, JIA Gen-liang1
（1.Life Science college of Northwest A&F University, Mangling 712100, Shanxi, China; 2.Forestry college of Northwest A&F University, Yangling 712100, Shanxi, China)viii
Abstract: The distributive feature of soil microorganism of Pinus tabulaeformis plantation forestland in Loess Plateau under different forest age has been studied. The results shows that quantitive variety of microorganism corresponding with forest age is not remarkable, but very remarkable with soil depth; the variety of bacterism quantity corresponding with soil depth abides by Quadratics Ratio model, the quantitive variety of actinomyces and fungi corresponding with soil depth fit to index function. Compared with the mixed stands of Pinus tabulaeformis and Amorpha fruticoca L. forest and wasteland, the quantity of soil bacterium of Pinus tabulaeformis plantation forestland is more than wasteland, but less than the mixed stands of Pinus tabulaeformis and Amorpha fruticoca L. forest. This turn into a basis to build mixed forest.
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