激光生物学报摘要, 更新时间: 2005年12月23日
  由美国生科集团 (BVTech, Inc.) 主办
  
激光扫描共聚焦显微镜观察胰蛋白酶对肺上皮细胞游离钙离子的影响
激光生物学报摘要 2005-5

王海燕，何韶衡，林珏龙 （汕头大学医学院变态反应学与炎症学研究所，中国广东 汕头515041）

摘 要：目的：研究PAR-2激动剂SLIGKV和tc-LIGRLO、胰蛋白酶及其抑制剂对H292肺上皮细胞[Ca2+]i的影响。方法：应用Fluo-3/AM 荧光标记技术和激光扫描共聚焦显微镜(LSCM) 检测不同因素处理的H292肺上皮细胞[Ca2+]i。结果：胰蛋白酶、SLIGKV、tc-LIGRLO均能引发H292细胞[Ca2+]i的增加，平均荧光强度分别比加入药物前增加267％，60％和37％。胰蛋白酶抑制剂大豆胰蛋白酶抑制剂（SBTI）和α1-抗胰蛋白酶（α1-AT）可以抑制胰蛋白酶诱导的细胞[Ca2+]i的增加。结论：PAR-2可以介导H292肺上皮细胞[Ca2+]i的释放增加，胰蛋白酶抑制剂可以抑制胰蛋白酶诱导的细胞[Ca2+]i的增加。


Effects of Free Calcium in H292 Cells through Protease-activated Receptor-2

WANG Hai-yan, HE Shao-heng, LIN Jue-long (Allergy and Inflammation Research Institute, Shantou University Medical College, Shantou 515041, China)

Abstract：Objective: To study the effects of PAR-2 agonists SLIGKV and tc-LIGRLO, trypsin and its inhibitors on intracellular calcium concentration [Ca2+]i in the H292 lung epithelial cell. Methods: Fluo-3/AM fluorescence technique and laser scanning confocal microscope (LSCM) were used to measure the intracellular calcium level after different reagents were added in Hanks of H292 cells. Results: Trypsin，SLIGKV, tc-LIGRLO could increase the intracellular calcium concentration [Ca2+]i in H292 cells. Compared with control, the average fluorescent intensities were increased 267％,60％,37％, respectively.Trypsin inhibitors soybean trypsin inhibitor(SBTI) and α1-antitrypsin(α1-AT) could inhibit trypsin induced the increase of [Ca2+]i in H292 cells. Conclusion: Activation of protease-activated receptor-2 induce the increase of [Ca2+]i in H292 cells. Trypsin inhibitors could inhibit trypsin induced the increase of [Ca2+]i in H292 cells.


 

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