激光生物学报摘要, 更新时间: 2005年12月23日
  由美国生科集团 (BVTech, Inc.) 主办
  
电离辐射对血管内皮细胞骨架蛋白F-actin影响的激光共聚焦显微镜观察(
激光生物学报摘要 2005-5

陈仕生1*，姚小武1，杨利和1，林珏龙2，杨海伟2 （1.汕头大学医学院第二附属医院口腔科，中国广东 汕头515041； 2.汕头大学医学院中心实验室，中国广东 汕头515041）

摘 要：目的：观察电离辐射对血管内皮细胞骨架蛋白F-actin影响，探讨血管内皮细胞电离辐射损伤的机理。方法：用Co60 γ射线对体外培养的血管内皮细胞进行0、2、4、6、8、10、12Gy照射，于照射后6小时后用激光共聚焦显微镜对其细胞骨架蛋白F-actin进行观察。结果：细胞骨架蛋白F-actin随着辐射剂量的增加而解聚增加，这种变化呈剂量依赖性。结论：电离辐射对血管内皮细胞骨架蛋白F-actin的破坏可能是血管内皮细胞辐射损伤机体机理之一。


The Observation of the Influence of Ionizing Radiation on the Image of F-actin in Vascular Endothelial Cells with Confocal Laser Scanning Microscope

CHEN Shi-sheng1, YAO Xiao-wu1, YANG Li-he1, LIN Jue-Long2, YANG Hai-wei2 (1.The Second Affiliated Hospital of Shantou University, Shantou 515041, Guangdong, China; 2.Shantou University Medical college Central Laboratory,Shantou 515041, Guangdong, China)

Abstract:Objective:To observe the influence of ionizibg radiation on the F-actin in vascular endothelial cells and investigate mechanism of ionizing radiation injury on vascular endothelial cells. Method: HUVE-12 cells was cultured in vitro and exposed to Co60 γ ray with 0、2、4、6、8、10、12Gy. The image of F-actin in HUVE-12 cells was observed with Confocal Laser Scanning Microscope at 6 hours after radiation. Results: radiation-induced depolymerization of F-actin microfilament stress fibres was dependent on radiation dose. Conclusion: radiation-induced depolymerization of F-actin microfilament stress fibres may be one of the mechanism of the radiation injury on vascular endothelial cells.


 

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