何家庆1,2, 黄训端1,2,黎承姬1,施忠辉3
（1.安徽大学生命科学学院，中国安徽 合肥 230039; 2.安徽省生态工程与生物技术重点实验室,中国安徽 合肥230039;3.安徽省绩溪县政府,中国安徽 绩溪 245300）
摘 要：针对栝楼子萌发困难的问题，本试验采用聚乙二醇（PEG）对栝楼种子进行处理，结果表明：15%PEG浸种24h，可有效调节渗透水势，改变种子生理生化反应特性，并保持淀粉酶活性连续上升，使可溶性蛋白质含量明显提高，可见PEG在加速大分子动员和提高代谢能力方面发挥了作用，从而促进植物体的组织器官形态建成和生长发育；同时，PEG处理提高了过氧化物酶（POD）活性，降低了丙二醛（MDA）含量，减少了自由基积累，减轻了浸种吸涨对膜系统的损伤，降低了不利因素的影响，提高了种子活力。
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Effects of PEG(Polyethylene Glyco) on seeds germination of richosanthes kirilowii
HE Jia-qing1,2,HUANG Xun-duan1,2,LI Cheng-ji1,SHI Zhong-hui3
(1.School of Life Sciences,Anhui University,Hefei 230039,Anhui,China;2.Anhui Key Laboratory of Eco-engineering and Bio-technique,Hefei 230039,Anhui,china;3.Government of Jixi County,Anhui Province,Jixi 245300,Anhui,China)
Absract:In this paper,15% PEG was applied to soak the seeds of Trichosanthes kirilowii for 24h in order to promote its germination.The results show that PEG could decrease osmotic pressure of water and change reactions of physiology and biochemistry in seeds effectively.For example,PEG could bring the high activity of amylase consecutively,and increase the content of dissoluble protein and the activity of,it might be said that PEG pretreatment could improve metabolic activity and reduce accumulation of POD (Peroxidase),but decrease the content of MDA (Malondialdehyde) in seeds. Based on the experiment free radical,and make cell membrane system less damage in course of absorbing water in seed,too.All these indicate that PEG can increase seed vitality and germination capacity.
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