激光生物学报摘要, 更新时间: 2005年12月23日
  由美国生科集团 (BVTech, Inc.) 主办
  
N+离子注入对干旱胁迫条件下甘草幼苗的SOD酶和CAT酶活性及丙二醛含量的影响
激光生物学报摘要 2005-6

潘燕,肖翔,吴李君,余增亮 (中国科学院等离子体物理研究所离子束生物工程学重点实验室, 中国安徽 合肥230031)

摘 要:以低能氮离子束为诱变源,对甘草种进行注入处理后,以萌发的幼苗为材料对其在干旱胁迫条件下保护酶活性及MDA含量变化进行研究。结果表明注入后甘草种的存活率呈现马鞍形曲线变化,其中在4.1×1016 N+/cm2剂量处达到鞍点,在剂量4.6×1016 N+/cm2处存活率高于对照。在干旱胁迫下,随着胁迫时间的延长,离子束注入后萌发的幼苗MDA含量明显低于对照。酶活结果显示,在干旱胁迫后期,剂量4.6×1016 N+/cm2处理后的甘草幼苗SOD和CAT酶活性都高于对照,说明该注入剂量处理甘草种有助于提高甘草幼苗的抗旱性能,SOD同工酶结果也证明该剂量的离子注入对干旱胁迫下同工酶谱带有所影响。


The Effect of N+ Ion Implantation on the MDA Content and the Activities of Superoxide Dismutase and Catalase of Liquorice Seedlings under PEG-induced Drought Stress

PAN Yan, XIAO Xiang, WU Li-jun, YU Zeng-liang (Key Laboratory of Ion Beam Bio-engineering, Institute of Plasma Physics, Chinese Academy of Sciences, Hefei 230031, Anhui, China)

Abstract: The effect of N+ ion implantation on the antioxidative system (SOD、CAT)and MDA content of liquorice seedlings(Glycyrrhiza uralensis Fisch )under PEG-induced drought stress was investigated in this study. The results showed survival ratio of liquorice seeds exhibited saddle-shaped curve. When the dose was 4.1×1016 N+/cm2, the survival ratio was the lowest, while the survival ratio was higher than that of control when the dose increased to 4.6×1016 N+/cm2. MDA measurement pointed out that the contents were significantly lower in seedlings irradiated by N+ ion beams of 4.6×1016 N+/cm2 dose when compared to control. The data also showed that SOD and CAT activity of irradiated seedlings by dose of 4.6×1016 N+/cm2 ion beam were higher than control, indicating that this implantation was useful in enhancement of drought resistance of liquorice seedlings. Result of SOD isoform pattern also demonstrates the biological effects of N+ ion implantation.


 

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