Abstract: Electrochemiluminescence technique has became a new gene detection method. It combined the sensitivity of electrochemiluminescence with the stability of traditional biology detection method. Compared with traditional gene detection technique, it is non-radioactive hazard, high sensitivity, and simplicity. It has been widely applied in gene analysis, detection of gene mutation and genetic disease. This paper introduced the basic principle of electrochemiluminescence and its application in gene detection. Ultimately, the bright future of electrochemiluminescence application in gene detection is prospected.