刘朝良1，王 磊1，梶浦善太2，中垣雅雄2
（1.安徽农业大学生命科学学院，中国安徽 合肥 230036；2.日本信州大学，上田 386-8567）
摘 要：天蚕卵黄原蛋白cDNA的全碱基序列由5720个碱基构成，一个开读框有5334个碱基序列，编码了包括由15个氨基酸组成的信号肽在内的共1778个氨基酸的蛋白质前体。天蚕和柞蚕卵黄原蛋白的同源性非常高，氨基酸序列达到92.6 %，碱基序列达到94 %。天蚕、柞蚕和家蚕的卵黄原蛋白的糖链附加位点(N-linkedglycosylationsite)：柞蚕有4个，家蚕有5个，天蚕和家蚕相同保存着5个。在N-末端区域，天蚕和家蚕有多聚丝氨酸区域和可被胰蛋白酶识别的RSRR部位；柞蚕虽然也具有多聚丝氨酸区域，但没有可被胰蛋白酶识别的RSRR部位。在C-末端区域里，大多数昆虫从GICG功能部位至C-末端结尾具有7~10个半胱氨酸(Cys)。鳞翅目的4种昆虫柞蚕、天蚕、家蚕和舞毒蛾(A.pernyi,A.yamamai,B.mori,L.dispar)都是7个半胱氨酸。
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Clone and Sequencing of Vitellogenin in Antheraea yamamai
LIU Chao-liang 1, WANG Lei1 , ZENTA Kajiura 2, MASAO Nakagaki2
(1. School of Life Science Anhui Agriculture University, Hefei 230036;2.Shinshu University of Japan , Uada 386-8567)
Abstract: The sequence of the Vitellogenin which contains 5270 bases and an open reading frame which contains 5334 bases was identified in the Antheraea yamamai. The 1778 amino acids concluding gasignal peptide of 15 amino acids were encoded by the ORF. The results of the homology analysis suggested the amino acid and base sequence of the Vg be highly conserved between Antherea peryni and Antherea yamamai. As far as the N-liked glycosylation site was concerned , there were 4 sites in A.peryni while A.yamamai and B.mori were 5 sites. In the N-terminal domain, the poly-serregions and RSRR sites recognized by proteinase except for A.peryni. In the C-terminaldomain, the majority of the insects had 7-10 Cys from the function site GICG to the end of C-terminal and there were 7 Cysirrespe ctivelyin A.peryni, A.yamamai, B.mori and L.dispar.
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