激光生物学报摘要, 更新时间: 2006年1月15日
  由美国生科集团 (BVTech, Inc.) 主办
  
果寡糖和甘露寡糖对断奶仔猪免疫机能的影响
激光生物学报摘要 2006-1

岳文斌，车向荣，臧建军，张晓萍 （山西农业大学动物科技学院, 中国山西 太谷030801）

摘 要：本研究探讨了果寡糖和甘露寡糖对断奶仔猪免疫机能的影响。试验采用随机区组设计法将48头健康、体重一致的35日龄断奶杜×长大仔猪分为四组，每组3个重复，每个重复4头进行为期四周的饲养试验。四个处理组分别为：基础日粮+0.5 %果寡糖（FOS组）、基础日粮+0.3 %甘露寡糖（MOS组）、基础日粮+0.45 %果寡糖+0.25 %甘露寡糖(F+M组)、基础日粮+75 mg/kg金霉素+40 mg/kg洛克沙生（ABT组）。试验结果表明：(1)寡糖组免疫器官指数较ABT组大（p﹥0.05）。（2）在断奶后第7天，FOS组（0.32±0.49）、MOS组（0.32±0.04）和F+M组（0.32±0.07）均较ABT组（0.20±0.01）显著提高了血清免疫球蛋白A（IgA）的浓度（p﹤0.05），同时FOS组（3.50±0.49）较ABT组（2.68±0.47）显著提高了IgG浓度（p﹤0.05）；F+M组显著提高了断奶后第21天IgG浓度（p﹤0.05）；MOS组显著提高了断奶后第21天IgM浓度（p﹤0.05）。（3）与ABT组相比，FOS显著提高了断奶后第7天胸腺（21.33±6.03对37.33±4.04）、脾脏（19.67±2.08对30.33±8.73）、淋巴结（24.67±4.16对37.67±2.52）CD4+T淋巴细胞百分数（p﹤0.05）；FOS组（21.33±4.04对32.00±5.29）、MOS组（21.33±4.04对37.33±3.21）、F+M组（21.33±4.04对32.00±3.60）显著提高了断奶后第28天胸腺CD4+T淋巴细胞百分数（p﹤0.05）；MOS组（20.67±3.51对29.67±5.51）显著提高了断奶后第7天胸腺CD8+T淋巴细胞百分数；F+M组（17.00±1.00对22.67±3.06）显著提高了断奶后第7天脾脏CD8+T淋巴细胞百分数（p﹤0.05）；各寡糖组对断奶后第28天CD4+、CD8+T淋巴细胞百分数影响不显著（p﹥0.05）；ABT组（34.33±5.03）在断奶后第7天较F+M组（20.67±6.43）显著提高了淋巴结CD8+T淋巴细胞百分数（p﹤0.05）；在断奶后第7天，FOS组（1.42±0.32）、MOS组（1.52±0.46）、F+M组（1.51±0.30）淋巴结CD4+/CD8+比值显著（P﹤0.05）大于ABT组（0.71±0.03）；在断奶后第28天， FOS组（1.36±0.21）和MOS组（1.34±0.16）脾脏CD4+/CD8+比值显著（P=0.01）大于ABT组（0.94±0.29），除此之外，各寡糖组CD4+/CD8+比值较寡糖组大（p﹥0.05），寡糖组间差异不显著。


Effects of Fructo-oligosaccharides and Mannan Oligosaccharides on Immune Response in Weaned Pigs

YUE Wen-bin,CHEN Xiang-rong,ZHANG Jian-jun,ZHANG Xiao-ping (College of Animal Science and Technoloy ,Shanxi, Agricultural University, Taigu 030801,Shanxi,China)

Abstract: The effects of Fructo-oligosaccharides(FOS) and Mannan-oligosaccharides (MOS) on immune response was studied in weaned pigs. Fourty-eight 35-d-old weaned Duroc×Landrace-Yorkshire pigs were divided into four groups with three replicates, each of which including 4 piglets, in a randomized block design. The four groups were fed with basic diet containing 0.5%FOS(group FOS), containing 0.3%MOS(group MOS), containing 0.45%FOS and 0.25MOS(group F+M), containing 75mg/kg Chlorcycline and 40mg/kg Roxarsone(group ABT), respectively. The results showed that: (1)The index of thymus, spleen, lymph node in group FOS, MOS, F+M were higher than that in group ABT(P﹥0.05). (2)In 7th day after weaned, the concentrations of immunoglobulin(IgA) in group FOS(0.32±0.08), MOS(0.32±0.04), F+M(0.32±0.07) significantly higher than in group ABT (0.20±0.01) (p﹤0.05), at the same time, the concentrations of IgG in group FOS(3.50±0.49) were higher than that in group ABT(2.68±0.47) (p﹤0.05); Compared with antibiotics, F+M increased the concentration of IgG markedly(p﹤0.05) in 21th day after weaned; MOS increased the concentration of IgM profoundly(p﹤0.05) in 21th day after weaned. (3)In 7th day after weaned, the percentages of CD4+ of thymus, spleen, lymph node in group FOS(37.33±4.04, 30.33±8.73, 37.67±2.52) were higher than in group ABT(21.33±6.03, 19.67±2.08, 24.67±4.16 accordingly) (p﹤0.05), the percentages of CD8+ of spleen in group F+M(22.66±3.05) were higher than in group ABT(17.00±1.00) (p﹤0.05), the percentages of CD8+ of thymus in group MOS(29.67±5.51)were higher than that in group ABT(20.67±3.51), the percentages of CD8+ of lymph node in group F+M(20.66±6.42) were lower than that in group ABT(34.33±5.03) (p﹤0.05); In 28th day after weaned, the percentages of CD4+ of thymus in group FOS(32.00±5.29), MOS(37.33±3.21), F+M(32.00±3.60) were higher than in group ABT(21.33±4.04) (p﹤0.05), there were no significant differences among group FOS, MOS, F+M and antibiotics(P﹥0.05) in CD8+ of thymus, spleen and lymph node(P﹥0.05); Though there were no significant difference among group FOS, MOS, F+M and ABT, CD4+/CD8+ ratio in group FOS, MOS,F+M was larger than that in group ABT, except that lympho node CD4+/CD8+ ratio in group FOS(1.42±0.32), MOS(1.52±0.46), F+M(1.51±0.30) was larger (p﹤0.05)than that in group ABT(0.71±0.03) in 7th day after weaned and spleen CD4+/CD8+ ratio in group FOS(1.36±0.21), MOS(1.34±0.16) was larger(p=0.01) than in group ABT(0.94±0.29).


 

Bioinformatics, sequence analysis; GCG; Life Science News; Drug Discovery.
生命科学进展新闻网
生科新闻网是反映生命科学研究进展的新闻网站。致力于为学术界和工业界的生命科学研究人员和大专院校师生提供及时， 准确的新闻和研究动态。为促进科技交流，尽其绵薄之力。
This is a web site of life science news in Chinese. It provides news and research trens in life sciences and drug discovery. This site is updated continuously throughout the day.