马洪雨, 姜运良, 郭金峰, 岳永生*
（山东农业大学动物分子遗传育种实验室 中国山东 泰安 271018）
摘 要：采用27对鲤微卫星引物对山东东平湖黄颡鱼进行全基因组扫描，结果有19对引物能获得稳定的扩增条带，其中有6个微卫星位点具有多态性。对这6个位点的扩增产物进行分析，结果显示：6个位点共检测到22个等位基因，每个位点的等位基因数从2个到6个不等; 平均基因纯合率为41.67％, 平均多态信息含量(PIC)为0.488，平均杂合度为0.5833 。这表明东平湖黄颡鱼种群结构合理，群体遗传多样性较丰富，种质资源尚处于安全状态。
|
|
Genetic Diversity Analysis on Pseudobagrus fulvidraco in Dong Ping Lake Using Microsatellite Markers
MA Hong-yu, JIANG Yun-liang, GUO Jin-feng, YUE Yong-sheng*
( Lab of Molecular Genetics and Animal Breeding, Shandong Agricultural University, Tai'an, 271018， Shandong)
Abstract: Twenty-seven pairs of microsatellite loci were applied to scan genomic DNA of Pseudobagrus fulvidraco in Dong Ping Lake, Shandong Province. Electrophoresis analysis showed that 19 pairs of primers reproducibly produced clear bands, and, of them 6 were polymorphic. There were totally 22 alleles of these microsatellite loci, and the number of alleles per locus ranged from 2 to 6. The average homozygosity, average polymorphism information content and average heterozygosity was 41.67 ％, 0.488 and 0.5833, respectively. The results indicated that the population structure of Pseudobagrus fulvidraco in Dong Ping Lake was reasonable and the breed resource was still in a safe status.
|
