欧 琳1a, 陈 荣1a, 庄伟建2, 肖华山1b
（1.福建师范大学 a.物理与光电信息学院;b.大学生物工程学院, 福建 福州350007; 2.福建农林大学作物科学院 福建 福州350007）
摘 要：本试验考察了用Ar+（488 nm）激光辐照对花生种子胚轴细胞形态结构以及根尖细胞染色体的影响。研究结果表明：在辐照功率密度为5.12 W/cm2，时间为10 s、25 s、55 s情况下，胚轴细胞均有不同程度的损伤；且均能诱发根尖细胞染色体畸变。处理时间延长，细胞破坏加剧，主要表现在细胞中膜系统、脂体、蛋白体和细胞核，出现了异形、合并或结构混乱等现象。染色体畸变类型也随之增加。各激光处理剂量均对花生种子萌发产生抑制作用。
|
|
Cytological Effect of Laser-irradiated On Peanut Seeds
OU Lin1 a，CHEN Rong1 a，ZHUANG Wei-jian2, XIAO Hua-shan 1b
(1.Fujian Normal University a.School of Physics and Optoelectronic Technology; b.Biological Engineering Institute, Fuzhou 350007; 2. Crop Science Institute, Fujian Agriculture and Forest University, Fuzhou 350007)
Abstract：In this present paper, dry seeds of peanut of cytological effects have been studied for Ar+ laser-irradiated at 488 nm. With laser 5.12 W /cm2 power density, there was difference in the plumule cell and cell nucleus, and the chromosomal aberration of peanut root tip cell was induced observed. With irradiating time added, the cells were destroyed serious, such as on the membrane system, fatbody, inhibition proteinbody and cell nucleus appeared irregular.,and the types of chromosomal aberration also increased. According to The seeds sprouting and growing, however, the dose of 60s treatment leads to a suitable dose.
|
