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Producing Transgenic Pig Empryos by Sperm Mediated Gene Transfer
XU Fei-liang1，XIAO Bin-nan2，LI Hong3，YAN Hai-feng2，DENG Yuan2，ZHANG Jian3*
(1.College of Animal Science in Hunan Agriculture University, Changsha 410128; 2.Hunan Institute of Animal and Veterinary Science, Changsha 410128; 3 Gene Knockout Lab, College of Life Science, Hunan Normal University, Changsha, 410081, China)
Abstract: Sperms which were removed of seminal fluid, incubated with exogenous DNA, so that they can intake exogenous DNA and fertilize with matured oocytes in vitro. The purpose is to develop a way to produce early transgenic pig embryos at lower costs. It indicated that sperm incubated with exogenous DNA could intake it and could be detected in embryos at different stage.
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