激光生物学报摘要, 更新时间: 2006年5月20日
  由美国生科集团 (BVTech, Inc.) 主办
  
60Co(射线辐射中国水仙的细胞学诱变效应
激光生物学报摘要 2006-2

高 健1,彭镇华2* (1.国际竹藤网络中心,国家林业局竹藤科学技术重点开放实验室,北京100102;2.中国林业科学研究院林业科学研究所,北京100091)

摘 要:用60Co(射线辐射中国水仙三年生鳞茎,首次研究了辐射后中国水仙鳞茎M1代形态损伤、剂量效应曲线、细胞学变化及其与形态变化的关系。结果表明,60Co(射线抑制细胞分裂,降低细胞分裂指数,引起中国水仙的形态损伤,并诱发中国水仙染色体畸变和核畸变,畸变类型丰富。在染色体畸变中染色体桥的发生频率较高,随着辐射剂量的增加染色体断片率提高。细胞核畸变中,微核发生率最高。核畸变率、染色体畸变率与剂量间存在显著的线性正相关,相关系数分别为0.9874、0.9829,线性方程表达式分别为Y=-0.085+1.0385X,Y=-9.6727+2.3697X。间期细胞微核率与分裂期染色体畸变率间呈正相关。


The Mutagenic Effects of 60Co( Ray Radiation to Chinese Narcissus bulb

GAO Jian1,PENG Zhen-hua2* (1.International Centre for Bamboo and Rattan, The Key Laboratory for Bamboo and Rattan of Chinese State Forestry Administration, Beijing 100102,China; 2 Chinese Academy of Forestry, Beijing 100091, China)

Abstract: Chinese narcissus bulbs were radiated by 60Coγ-ray with different dosages. The injury effects of morphology and cytology in M1 generation, the dose effects and the relationship among them were studied. The results indicated that 60Co(-ray was an effective mutagenesis for ornamental plant breeding. It induced the chromosomal aberration and nuclear aberration. There were abundant aberrant types. In chromosomal aberration the frequency of chromosomal bridge was the most. In nuclear aberration the frequency of micronucleus was the most. The relationship between different dosages and frequencies of chromosomal aberration and nuclear aberration of root tip cells in M1 generation was fitted in with the model Y=-0.085+1.0385X, Y=-9.6727+2.3697X respectively. The correlation coefficient was 0.9829 and 0.9874 respectively.


 

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