激光生物学报摘要, 更新时间: 2006年5月20日
  由美国生科集团 (BVTech, Inc.) 主办
  
根据12S rRNA和Cyt b基因部分序列鉴定药用猴骨样品
激光生物学报摘要 2006-2

刘辉， 吴孝兵* （安徽师范大学生命科学学院　重要生物资源保护与利用安徽省重点实验室，芜湖 241000）

摘要：本文利用线粒体12S rRNA基因和Cyt b基因部分序列，通过DNA序列比对的方法，对亳州市药品监督管理局送检的三件猴骨样品（BZ01、BZ02和BZ03）进行了分子鉴定。结果表明，BZ01号样品为藏酋猴（Macaca thibetana），BZ02和BZ03号样品为猕猴（Macaca mulatta）。通过本文的研究，作者还提出，在利用DNA序列比对法进行近缘物种的鉴定时，不同种群间的DNA序列变异，可能对结果造成一定的影响。


Identification of Pharmaceutical Monkey Skeletons Samples based on 12S rRNA & Cyt b gene partial sequences

LIU Hui， WU Xiao-bing* (Conservation and Utilization of Important Biological Resources of Anhui Key Laboratory, College of Life Science, Anhui Normal University, Wuhu 241000, China)

Abstract: The Medical Supervise Bureau of Bozhou City sent us three animal samples (BZ01, BZ02 &BZ03) for identification, which were unknown dried monkey skeletons infused by medicine or spice. The DNA sequences alignment method was adopted for the identification in this study based on the mitochondrial 12S rRNA & Cyt b gene partial sequences. The results show that the sample BZ01 is Tibetan macaque (Macaca thibetana) and the sample BZ02 & BZ03 are rhesus monkey (Macaca mulatta). Besides, it was pointed out when using the DNA sequences alignment method to identify related species, DNA sequence variations among different populations may influence the result to a certain extent.


 

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