激光生物学报摘要, 更新时间: 2006年5月20日
  由美国生科集团 (BVTech, Inc.) 主办
  
山西瘦肉型猪（SD-Ⅲ系）基因组DNA的AFLP检测研究
激光生物学报摘要 2006-2

朱文进1、2，苏咏梅1，关学敏1，岳文斌2 ，姜玉锁2，张美俊2， 张桂贤2，郭传甲2* （1.河北科技师范学院 动物科学系，河北 昌黎 066600； 2. 山西农业大学 动物科技学院，山西 太谷 030801）

摘 要:本研究用分子标记AFLP对山西瘦肉型猪（SD-Ⅲ系）进行纯度检测，旨在为评价该猪种的遗传特性提供相关参数。实验共用8条引物，对25头猪进行了基因组DNA的分析，共获得171个AFLP标记，单引物获得的标记数在3个~15个之间，群体相似系数为0.928（0.892~0.978）；遗传距离为0.072(0.022~0.108)。结果表明：AFLP适宜于基因组DNA遗传结构检测；SD-Ⅲ系猪纯度较高。


Genetic Monitoring of Genomic DNAs in Shanxi Lean Meat Pigs (SD-Ⅲ Line) by AFLP

Zhu et al

Abstract:This paper used DNA fingerprinting techniques AFLP to detect the purity of Shanxi lean meat pigs in molecular level, it provides a relational parameter for appraising genetics character of SD-ⅢLine. 8 AFLP primers were used, genomic DNAs were from 25 pigs, 171 AFLP markers were obtained, the obtained marker numbers of individual primer were between 3~15. The similarly index of population in Shanxi local pigs was 0.928 (0.892~0.978 ). The results showed that AFLP is fit for genetic detection of genomic DNAs, and that the pure degree of SD- Ⅲ line is higher.


 

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