彭益强a，张 燕a，庄月娥a，方柏山a，张文珍b
（华侨大学 a.生物工程系，b.信息科学与工程学院, 福建 泉州362021）
摘 要：采用激光复合诱变方式筛选产中性植酸酶的大肠杆菌(Escherichia Coli)、枯草杆菌(Bacillus subtilis)，混合波长λ=1.06+0.53 μm，辐照时间1 min,辐照功率15 w；酶的细胞定位实验表明，所产中性植酸酶是胞外酶；酶学特性测定表明，大肠杆菌所产植酸酶酶反应最适pH为7.4，最适温度为37 ℃，枯草杆菌所产植酸酶反应最适pH为7.4，最适温度为37 ℃；酶学性质分析表明，大肠杆菌所产植酸酶具有一定的pH适性和一定的温度耐受性。
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Screening Neutral phytase Producing Strains by Laser Compound Mutagenesis
PENG Yi-qianga , ZHANG Yana, ZHUANG Yue-ea, FANG Bai-Shana, ZHANG Wen-Zhenb
(Huaqiao University a.Department of Bioengineering;2.College of information science and Engineering ,Quanzhou 362021,Fujian,China)
Abstract: Neutral phytase producing strains were screened by laser compound mutagenesis, mix wavelength 1.06+0.53 μm, irradiated time 1 min, irradiated power 15 w; The orientation test show that the produce phytase was extracellular-enzyme; Enzymology property mensurate show that E. Coli produce phytase optimal pH value was 7.4, optimal temperature was 37℃, B. subtilis produce phytase optimal pH value was 7.4, optimal temperature was 37℃; Enzymology property analysis show that E. Coli produce phytase has some extent pH flexibility and thermal endurance.
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