Abstract：Cellular engineering is one of the four techniques of biotechnology, and as the core of cellular engineering, the cell fusion technique has obtained outstanding achievements in many fields such as agriculture, medicine and environmental protection. Its application is still increasing in number. The improvement of the cell fusion technique involves the fusion agent, new methods and the cells used in fusion. Now new cell fusion methods are resorting to the combined usage of physical and chemical ways to develop a simple and convenient, easy quantification and at the same time can increase the fusion rate. All these aspects were discussed in this paper centered around the history of the cell fusion technique.