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小鼠睾丸凋亡基因MSRG-11的人类同源基因SPATA17的克隆
激光生物学报摘要 2006-3
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邓 云①, 王 建① , 胡亮杉, 卢光琇*
（中南大学 人类生殖与干细胞工程研究所, 中国湖南 长沙 410078）
摘 要: 利用生物信息学方法结合实验手段克隆了一个在睾丸组织中特异高表达的小鼠生精细胞凋亡相关基因Spata17的人类同源基因SPATA17（GenBank登录号为AY963797）。应用生物信息学分析显示该基因定位在染色体1q41，包含11个外显子,内含子和外显子交界区均符合gt-ag规则；该基因开放阅读框为1083bp编码一个由361个氨基酸组成的、分子量为43.49kD、等电点为9.71、含有三个保守IQ功能域的蛋白；该基因5'端从-176bp至-659bp含有一个CpG岛，-828bp至-928bp有一个启动子；对SPATA17编码蛋白质进行生物信息学分析,无跨膜区,无信号肽序列，推测为一非分泌性蛋白。多组织和Northern blot结果显示该基因只在睾丸组织中特异高表达，转录本大小为2.0kb。总之，研究表明SPATA17在睾丸组织生精细胞凋亡起重要作用。
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Cloning of SPATA17, A human Testis-Specific Apoptosis Gene Homologous to MSRG-11 in Mouse
DENG Yun, WANG Jian, HU Liang-sha , LU Guang-xiu *
(Institute of Human Reproductive and Stem Cell Engineering, Central South University, Changsha 410078, Hunan)
Abstract: SPATA17 gene, homologue to mouse MSRG-11 gene, was cloned by informatics analysis. The cDNA mapped to chromosome 1q41 in the human genome is made up of 11 exons and the exon-intron boundaries obey to the AG/GT rule. The gene contains a 1083 bp open reading frame encoding 361 amino acid sequences with theoretical molecular weight of 47.49 KD and isoelectric point of 9.75. The protein encoded by this gene contains three highly conserved IQ motifs and is a new member of IQ protein family. One CpG island is located in the gene from site -176 to -659. A typical promoter is also predicted from site -828 to -928. According to the computer-aided analysis of the putative protein encoded by the SPATA17, no transmembrane region and no signal peptides are found in the protein. Multi-tissue RT-PCR and northern results show that the SPATA17 transcript of 2.0 kb was detected strongly in adult human testis only. In conclusion, this study predicts its important role in spermatogenesis cell apoptosis
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Bioinformatics,
sequence analysis; GCG; Life Science News; Drug Discovery.
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