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The Studies on Mutagenesis of Thermomonospora fusca with (60) Co Gamma Rays and its Application in the Fermentation of Excrement
MAO Xiang-bing1，2, XIAO Bing-nan1*, LIU Yi2, YANG Ai-zhi3, ZHOU Wang-ping1, QIU Mei-zhen1
(1. Hunan institute of animal and veterinary science, Changsha 410131 Hunan; 2.College of Animal and Sciences-Technology , Hunan agricultural university , Changsha 410128,Hunan; 3.The agricultural technology stage in Baima Bridge Village, Ningxiang County, Hunan Province, Ningxiang 410600 Hunan)
Abstract: In this experiment, Thermomonospora fusca was chosen as an original strain for screening high yield cellulase mutants. The spore suspension of organism was treated with 60Co gamma rays. A high cellulase-producing mutant strain AV5 was obtained by screening using the methods of transparent zone and shaking culture. The activity of mutant strain AV5 was enhanced for 1.8 times comparing with that of the original strain. After fermentation the excrement of cattle, the reducing rate of the crude cellulose of excrement is 32.95 %.
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