激光生物学报摘要, 更新时间: 2006年7月23日
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60Coγ射线对高免卵黄抗体(IgY)活性和理化特性的影响
激光生物学报摘要 2006-3

霍乃蕊1a , 柴桂珍2 , 郑明学1b﹡ , 韩克光1b (1.山西农业大学 a.食品科学与工程学院; b动物科技学院,山西 太谷 030801; 2.山西省兽药检测所,山西 太原 030000;)

摘 要:利用60Coγ射线照射卵黄抗体,分析了60Coγ射线对IgY的效价、纯度、热稳定性、耐酸性以及酶水解试验等理化特性的影响和不同温度下的保存时间。结果表明照射达10kGy时,对IgY的效价和纯度无影响;对照组和照射组热稳定性、耐酸性无差异,但照射组更易被酶水解;照射后的IgY明显延长了保存期。


Effect of 60Co γ Ray on Activity and Physic-chemistry Characters of Intensely Immunized Egg Yolk(IgY)

HUO Nai-rui1a CHAI Gui-zhen2 ZHENG Ming-xue1b﹡ HAN Ke-guang1b (1. Shanxi Agricultral University a. College of food Science and Engineering ; b. College of Animal Science and Technology, Taigu 030801,Shanxi; 2. Animal Medicine Monitoring Institute, Taiyuan 030000, Shanxi)

Abstract: The Immunized Egg Yolk was irradiated by 60Coγ ray, and then we investigated the effect of 60Co γ ray on antibody titer、purity、heat stability、acid resistance、enzymatic hydrolysis and other activity and physic-chemistry characters of it. When irradiation was up to 10kGy, the results showed that there was no effect on antibody titer and purity of IgY;there was no differece in heat stability and acid resistance beween the irradiated group and the control group,but the irradiated group was more easily hydrolyzed by enzyme;The retention period of irradiated IgY was obviously prolonged.


 

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