激光生物学报摘要, 更新时间: 2006年9月2日
  由美国生科集团 (BVTech, Inc.) 主办
  
羊驼线粒体12srRNA/tRNA-Val/16rRNA
激光生物学报摘要 2006-4

基因序列测定及分子进化研究 姜俊兵， 张巧灵， 贺俊平， 耿建军 ，董常生* （山西农业大学动物科技学院，山西 太谷 030801）

摘 要：本试验对GaneBank中登录的部分骆驼科动物线粒体12srRNA/tRNA-Val/16rRNA基因序列进行同源性比较，并借助DNAstar软件设计引物，扩增并进行序列分析，旨在揭示其遗传变异的规律。PCR条件优化后成功扩增出长度为1014 bp的DNA片段，blastn分析显示，该片断与骆驼科动物的同源性高于95%，所获得片断包括30 bp的12srRNA基因部分序列，71bp的tRNA-Val基因全序列和913bp的16srRNA基因部分序列。利用RNAstructure 4.2 RNA分析软件绘制了部分骆驼科动物的线粒体tRNA-Val推导性二级结构图，比较结果显示，羊驼线粒体tRNA-Val氨基酸臂和反密码子环呈属间特异性遗传。


Sequencing Analysis of Mitochondrion 12srRNA/tRNA-Val/16rRNA and Study on the Molecular Evolution of Alpaca

JIANG Jun-bing , ZHANG Qiao-ling, HE Jun-ping , GENG Jian-jun , DONG Chang-sheng* (College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, Shanxi ,China )

Abstract: The purpose of this study was analysis and tracking the heredity stability of Alpaca through amplifying special fragment from mitochondrion genome. A pair of primer was designed according to those sequences of Camelidae animal submitted in GaneBank and a fragment of 1014 bp was cloned. Analyzing by blastn program of NCBI, the fragment includes: 30bp 12srRNA partial sequence, 71bp tRNA-Val complete sequence and 913bp 16srRNA partial sequence, and the homolog is more than 95 % compared with other Camelidae animals. The secondary structure of tRNA-Val was mapped using RNAstructure 4.2. The results showed that the amino acid arm and anticodon loop of tRNA-Val is conserved among genus.


 

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