激光生物学报摘要, 更新时间: 2006年9月2日
  由美国生科集团 (BVTech, Inc.) 主办
  
Alpha粒子和柴油机尾气颗粒提取物
激光生物学报摘要 2006-4

共同处理哺乳动物细胞的协同毒性作用 朱 键，许 安，吴李君，余增亮 （中科院离子束生物工程重点实验室，安徽 合肥 230031）

摘 要：为研究两种有害环境因子同时作用对于人类健康的影响，以alpha粒子和柴油机尾气颗粒提取物（diesel particulate extract, DPE）共同处理皮肤成纤维细胞(AG1522)为实验模型，对细胞毒性、遗传毒性进行研究。实验发现和未处理的对照组相比，1 cGy alpha粒子和20 μg/mL DPE处理24 h共同作用于细胞能够导致细胞活力下降40 ％左右，微核形成率上升2倍多；而1 cGy alpha粒子单独辐照时细胞活力下降14.19 ％，微核形成率上升了36.84 ％，20 μg/mL DPE单独处理24 h时细胞活力下降17.29 ％，微核形成率上升了73.68 ％。实验结果表明:这两种环境因子共同作用于哺乳动物细胞时，对于细胞的毒性不是简单的叠加，而是表现出一种协同效应。


A Synergistic Toxic Effect in Mammalian Cells Induced by Co-treatment of Alpha Particle and Diesel Particulate Extract

ZHU Jian , XU An , WU Li-jun , YU Zeng-liang ( Key Laboratory of Ion Beam Bioengineering, Chinese Academy of Sciences, Hefei 230031, Anhui )

Abstract: In order to investigate the influence on the health of human beings of two environmentally detrimental factors working together, AG1522 cells co-treated by alpha particles and diesel particulate extract (DPE) were employed as an experimental model to explore their cytotoxicity and genotoxicity. It was found that, in comparison with control, a combination of 1cGy alpha particle and 24 hour 20 μg/mL DPE treatment led to a 40 percent reduction in cell viability and 2-fold increase in micronuclei formation, whereas either 1 cGy alpha particles radiation alone reduced cell viability by 14.19 % and increased micronuclei formation by 36.84 %, or 24-hour 20μg/mL DPE treatment alone diminished cell viability by 17.29 % and raised micronuclei formation by 73.68 %. Our current work demonstrates that the toxicity


 

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