武 标１ ,武 威2 , 张 千１ , 赫英军3 , 程 颖１
(1.辽宁大学生命科学系，辽宁 沈阳110036 ；２.辽宁大学环境科学系，辽宁 沈阳110036 ；
3. 沈阳味精厂清原分厂，抚顺113300)
摘 要：从污染的谷氨酸发酵废液中分离纯化噬菌体，并以高滴定度侵染生产敏感菌，借助菌的自发突变筛选出抗噬突变株，再运用简单便利的ＵＶ、ＮＴＧ复合诱变手段和初、复筛方法，最后选育出抗噬谷氨酸高产菌株。其过程简单、便利，可靠性高，是选育抗噬谷氨酸高产菌株的好方法，对发酵行业具有指导意义。曾选育到抗噬谷氨酸高产菌株，其瓶发酵产量比对照提高21.4 % 。
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Screen the Phage-resistant and High-producing Strain of Glutamic Acid
WU Biao1, WU Wei2, ZHANG Qian1, HE Yingjun3, CHENG Ying1
(1.Department of Life Science , Liaoning University, Shenyang, 110036;
2.Department of Environmental Science , Liaoning University, Shenyang, 110036;
3.Qing Yuan Gourment Pouder Factory, Fushun,113300)
Abstract: With infected phage-sensitive producing strains by phages isolated from waste-liquid of glutamic acid fermentation, the phage-resistant mutants were isolated with spontaneity mutation. The high-producing strains were screened by simple and convenient method with mixed UV and NTG treatment. It is effective method for screening phage-resistant and high-producing strain because the processing is simple, convenient and reliable. With this method, we got the high-producing and phage-resistant strain which increased 21.4%than the control in shaking-flask culture.
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