|
|
紫外线和60Coγ射线对钝顶节旋藻(Arthrospira platensis)的诱变效应
激光生物学报摘要 2006-5
|
曹媛媛, 甘旭华, 赵良侠, 夏觅真, 刘广金, 唐欣昀(
（安徽农业大学生命科学学院，安徽 合肥230036）
摘 要: 将对数期的钝顶节旋藻 (Arthrospira platensis ) (原名钝顶螺旋藻，Spirulina platensis)A9菌株用超声波40 s预处理破碎成2个~4个细胞大小的片断，分别用不同剂量的紫外线(UV)和60Coγ射线处理，诱变后经3小时避光预培养分别接种于液体和固体培养基进行培养。固体培养时UV照射70 s和60Coγ照射3500 Gy后无菌体存活，而在液体培养中高剂量处理的样品可以部分恢复生长。将诱变后的菌液分别加入20 μg·ml-1的ρ-氟苯丙氨酸(ρ-fluorophenylalanine, FPA)和20 μg·mL-1的刀豆氨酸(L-canavanine sulphate , CS)，放入光照培养箱中预培养3 天~4天后涂于含相同浓度的氨基酸类似物FPA和CS平板，培养30天后计算抗氨基酸类似物突变株的突变率。UV对A9菌株的完全致死剂量LD和存活率37 %时的剂量D37值分别为70 s和22 s，LD/ D37=3.18。A9菌株经UV诱变25 s时存活率为28.7 %， 抗FPA和抗CS突变率分别为2.31×10-3和1.50×10-3， 最大诱变效应比（MME）分别为48.53和 52.63。60Co-γ射线对A9菌株的LD和D37值分别为3500 Gy、1250 Gy, LD/ D37=2.8。 60Coγ射线显著提高A9菌株的突变率，当诱变剂量为2000 Gy、存活率为10.49 %时A9菌株突变率最高，抗FPA和抗CS突变率分别为5.07×10-3和0.964×10-3，最大诱变效应比（MME）分别为241.43和74.15。60Coγ射线对钝顶节旋藻A9菌株的损伤比UV造成的损伤强烈（低的LD/ D37值），比UV具有较大的诱变效应（高MME值）。采用两种诱变剂获得的抗FPA突变率都要高于抗CS突变率。通过诱变获得了大量的抗氨基酸类似物突变株，为遗传重组研究提供携带重要遗传标记的材料。
|
|
Mutagenic effect of UV and 60Coγ ray on A9 strain of Arthrospira platensis
CAO Yuan-yuan, GAN Xu-hua, ZHAO Liang-xia ,
XIA Mi-zhen, LIU Guang-jin, TANG Xin-yun(
(Anhui Agricultural University, Life Science College, Hefei 230036, Anhui, China)
Abstract: Arthrospira platensis A9 strain in log phase was broken to 2 cells ~4 cells fragments by 40 s pretreatment of ultrasonics, the sample was mutagenized by ultraviolet (UV) and 60Coγray, and then the samples were inoculated into liquid and solid Zarrouk Medium after 3 h pre-incubation. With the dosage of radiation increasing the survival ratio of samples were decreased dramatically and under the condition of UV for 70 s and 60Coγray for 3500 Gy no growth occurred on plates, whereas the growth of the samples with high dosage treatment could somehow resumed in liquid. The samples mutagenized were inoculated to liquid medium with 20 μg·mL-1ρ-fluorophenylalanine (FPA) and 20 μg·mL-1 L-canavanine sulphate (CS) respectively in a light incubator for 3 day ~ 4 day, and then spread on plate with the same concentration of FPA and CS. The resistant mutation rates were calculated after growing for about 30 day.
|
|
|
|
|
|
Bioinformatics,
sequence analysis; GCG; Life Science News; Drug Discovery.
|
|
